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Effect of Kelor Leaves (Moringa oleifera) in Liver Function of Streptozotocin induced Diabetic Sprague dawley Strain Male Rats Nashih Abdillah; Devy Ariany; Muniroh . Muniroh
The Avicenna Medical Journal Vol 3, No 2 (2022)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v3i2.27055

Background: Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia. Prolonged hyperglycemia can damage various organs of the body including the liver. Moringa oleifera leaves extract contain flavonoids which can decrease blood glucose levels and act as a hepatoprotector. To evaluate its hepatoprotective activity, liver function marker Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvate transminase (SGPT ) was used. In this study, we aimed to determine the effect of Moringa oleifera leaves extract on the liver function test of male Sprague dawley rats that have DM due to streptozotocin induction.Methods: This was experimental study conducted from February 2019 to July 2019 at Faculty of Medicine Laboratory, UIN Syarif Hidayatullah, Jakarta. This study conducted in 25 male Sprague dawley rats divided into 5 groups, those were negative controls group, positive controls group, and 3 treatment groups which given oral Moringa oleifera leaves extract at doses of 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW, respectively, for 14 days. Samples were collected on day 26th to measure SGOT and SGPT. Blood glucose levels were assayed on day 6th and 26th.Results: There was no significant decrease in SGOT (p=0.069) and SGPT (p=0.345) levels in the administration of Moringa oleifera leaves extract to male Sprague dawley rats with DM induced by streptozotocin at a dose of 200 mg/KgBW and 400 mg/KgBW.Conclusions: Moringa oleifera leaves extract did not significantly decrease SGOT and SGPT.

Effect of Kelor Leaves (Moringa oleifera) in Liver Function of Streptozotocin induced Diabetic Sprague dawley Strain Male Rats Nashih Abdillah; Devy Ariany; Muniroh . Muniroh
The Avicenna Medical Journal Vol 3, No 2 (2022)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v3i2.27055

Background: Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia. Prolonged hyperglycemia can damage various organs of the body including the liver. Moringa oleifera leaves extract contain flavonoids which can decrease blood glucose levels and act as a hepatoprotector. To evaluate its hepatoprotective activity, liver function marker Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvate transminase (SGPT ) was used. In this study, we aimed to determine the effect of Moringa oleifera leaves extract on the liver function test of male Sprague dawley rats that have DM due to streptozotocin induction.Methods: This was experimental study conducted from February 2019 to July 2019 at Faculty of Medicine Laboratory, UIN Syarif Hidayatullah, Jakarta. This study conducted in 25 male Sprague dawley rats divided into 5 groups, those were negative controls group, positive controls group, and 3 treatment groups which given oral Moringa oleifera leaves extract at doses of 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW, respectively, for 14 days. Samples were collected on day 26th to measure SGOT and SGPT. Blood glucose levels were assayed on day 6th and 26th.Results: There was no significant decrease in SGOT (p=0.069) and SGPT (p=0.345) levels in the administration of Moringa oleifera leaves extract to male Sprague dawley rats with DM induced by streptozotocin at a dose of 200 mg/KgBW and 400 mg/KgBW.Conclusions: Moringa oleifera leaves extract did not significantly decrease SGOT and SGPT.

Efektivitas Ekstrak Daun Kelor (Moringa oleifera) Terhadap Kadar Trigliserida dan Glukosa Darah Sewaktu pada Tikus Jantan Galur Sprague Dawley Diabetes Melitus Muniroh, Muniroh; Masnunah, Masnunah; Ariany, Devy
Journal of Islamic Medicine Vol 8, No 2 (2024): JOURNAL OF ISLAMIC MEDICINE EDISI SEPTEMBER 2024
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v8i2.29672

Background: According to data from the International Diabetes Federation (IDF), Indonesia ranks 5th in the world for the highest number of diabetes mellitus cases, with numbers predicted to continue rising. Dyslipidemia is one of the complications of diabetes mellitus, potentially leading to coronary heart disease. Kelor leaves (Moringa oleifera) contain flavonoids, which may reduce blood glucose and triglyceride levels. Objective: This study aims to evaluate the effect of Moringa oleifera leaf extract on blood glucose and triglyceride levels in male Sprague dawley rats. Methods: This experimental study involved 24 male Sprague dawley rats. Blood glucose and triglyceride levels were measured before and after administering Moringa oleifera leaf extract. The Shapiro-Wilk test assessed data normality, followed by paired t-tests, One-way ANOVA, and Pearson correlation. Results: Moringa oleifera leaf extract at a 400 mg/kg BW dose significantly reduced blood glucose levels (p=0.017). No statistically triglyceride levels decreased in all groups (p=0.195). Conclusion: Moringa oleifera leaf extract can significantly lower blood glucose levels at a dose of 400 mg/kg BW. Further research is needed to investigate the effects of Moringa oleifera on triglyceride levels

Efek Ekstrak Nigella sativa Terhadap Histopatologi Pankreas Dan Hepar Pada Tikus Model Diabetes Melitus Abdul Rasyid; Devy Ariany; Luluk Hermawati; Nurlaely Mida Rachmawati; Hilizza Awalina Zulfa
Jurnal Gizi Kerja dan Produktivitas Vol 6, No 1 (2025): May
Publisher : Fakultas Kedokteran Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/jgkp.v6i1.32584

Diabetes melitus (DM) merupakan gangguan metabolik kronis yang ditandai oleh hiperglikemia akibat defisiensi absolut maupun relatif insulin, atau resistensi terhadap kerja insulin. Kondisi ini tidak hanya memengaruhi metabolisme glukosa, tetapi juga dapat menimbulkan komplikasi sistemik, termasuk kerusakan organ vital seperti hepar. Streptozotocin (STZ), suatu agen antimikroba yang diisolasi dari Streptomyces achromogenes, dikenal memiliki sitotoksisitas selektif terhadap sel β pankreas, sehingga sering digunakan untuk menginduksi model diabetes eksperimental melalui destruksi sel β. Di samping terapi farmakologis konvensional menggunakan obat antidiabetes oral (OAD), pendekatan alternatif seperti penggunaan fitoterapi telah banyak diteliti karena potensi efek terapetiknya dalam mengelola DM dan komplikasinya. Salah satu tanaman herbal yang berpotensi adalah habbatussauda (Nigella sativa) yang mampu memperbaiki kerusakan sel β pankreas serta jaringan hati. Penelitian ini bersifat eksperimental dan bertujuan mengevaluasi efek terapi habbatussauda (HS) terhadap gambaran histopatologis pankreas dan hati tikus yang diinduksi dengan STZ. Hasil menunjukkan bahwa pemberian HS dengan dosis 300 mg/kgBB/hari dapat memperbaiki struktur sel β pankreas, ditandai dengan bentuk nukleus yang lebih membulat dan susunan sel yang lebih teratur, serta menormalkan ukuran nukleus hepatosit. Kesimpulannya, habbatussauda mampu memberikan efek perbaikan pada jaringan pankreas dan hati tikus yang diinduksi STZ, berdasarkan hasil pewarnaan Hematoxylin-Eosin (HE).

STUDI HISTOPATOLOGI PENGARUH PEMBERIAN DMBA SECARA TOPIKAL PADA ORGAN HATI MENCIT C3H/HEJ Ariany, Devy; Rahman, Abd; Rachmawati, Nurlaely Mida; Hermawati, Luluk; Irawati, Nur Bebi Ulfah
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran Vol. 10 No. 1 (2025): Maret
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/mtj.v10i1.1892

Optimalisasi Waktu Fiksasi Menggunakan Formalin 10% Pada Ginjal, Hepar, dan Pankreas Tikus Sprague Dawley Untuk Pewarnaan Haematoxylin-Eosin Alwi, Muhammad Azharan; Rachmawati, Nurlaely Mida; Ariany, Devy; Hermawati, Luluk; Zulfa, Hilizza Awalina
Calvaria Medical Journal Vol 3 No 2 (2025): Edisi Desember 2025
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/kp751w77

Background: Histotechnology is a series of procedures used to prepare histological specimens for microscopic analysis. One of the critical steps in this process is fixation, which serves to preserve tissue structure and prevent changes in shape or size. However, excessive fixation duration may lead to over-hardening of tissues, resulting in reduced quality of histological observations. Objectives: To evaluate the feasibility of two week formalin 10% fixation period on histological quality of kidney, liver, and pancreas tissues. Methods: After a 14-day adaptation period, male Sprague Dawley rats were anesthetized, necropsied, and their kidney, liver, and pancreas were fixed in 10% formalin. Histotechnical procedures included graded alcohol dehydration, toluene clearing, paraffin embedding, 6 µm sectioning, and HE staining. Preparations were observed using an Olympus BX41 microscope at magnifications of 4x–40x. Results: Two-week fixation caused structural damage to the kidney, liver, and pancreas. Damage observed included glomeruli and tubules in the kidney, the portal triad structure in the liver, and the Langerhans islet cells in the pancreas. Statistical results (if any) should be included here. Conclusions: Fixation for two weeks did not produce adequate histological preparations, and therefore, it is not recommended as a reference for developing histotechnology SOPs in related laboratories.

Aggressiveness of Indonesia Triple Negative Breast Cancer Driven by Programmed Death Ligand-1 and Tumor Microenvironment Sari, Dianidya Kumandang; Fitri Hapsari , Rr. Ayu; Ariany, Devy; Sakaraga, Deamon
The Avicenna Medical Journal Vol. 6 No. 2 (2025): The Avicenna Medical Journal
Publisher : Faculty of Medicine, UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v6i2.48991

Background: Triple-negative breast cancer (TNBC) is one of the most aggressive breast cancer subtypes in Indonesia. The expression of PD-L1 1 and TME characteristics play essential roles in both immune system avoidance and medical treatment results. The Indonesian medical field lacks a comprehensive analysis of local TNBC research data because new studies continue to emerge. Methods: The research followed PRISMA guidelines and PECO framework for analysis. A total of 22 records were identified; 10 studies met eligibility, and 6 studies were included in the final synthesis. Results: The studies showed a wide range of PD-L1 expression in tumor cells, from 21% to 93.5%, depending on antibody clone (SP142, SP263, 22C3) and scoring threshold. The studies showed that most tumors had high-grade characteristics between 42% and 88% while displaying high proliferative rates with Ki-67 ≥20% in more than 80% of cases. The three most common metastasis locations in patients were the lungs, bones and then the brain. The survival rates of patients improved when their CD8/CD163 and CD4/FOXP3 ratios increased. The survival outcomes of patients worsened when their PD-L1 expression levels or mRNA expression increased. Conclusion: Indonesian TNBC shows aggressive pathological characteristics with variable PD-L1 expression and diverse tumor microenvironments. PD-L1 alone does not provide consistent prognostic value and is best interpreted together with immune-cell ratios and TIL density. Establishing standardized biomarker evaluation and adopting immune-based molecular classification could improve the precision of immunotherapy strategies in Indonesia. Keywords:Triple-negative breast cancer, PD-L1, tumor microenvironment, immune ratios, metastasis, Indonesia

Preliminary Study on Long Fixation in Histological Preparations of Internal Organs of Sprague Dawley Rats Galang Prahanarendra; Devy Ariany; Nurlaely Mida Rachmawati; Luluk Hermawati; Ghea Farmaning Thias Putri
International Journal of Cell and Biomedical Science Vol 4 No 12 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i12.77

Histotechnology is a series of processes ranging from tissue handling to the preparation of slides that can be observed under a microscope. One crucial stage in this process is fixation, which serves to preserve the structure and morphology of the tissue as close as possible to its original physiological state. However, prolonged fixation duration may lead to tissue hardening, dissolution, and structural damage. This study aims to obtain supporting data for the development of a standard operating procedure (SOP) in histotechnology that can be applied in the animal house and histology laboratories of the Faculty of Medicine, Syarif Hidayatullah State Islamic University Jakarta. The results showed that fixation for three weeks caused morphological damage to the kidney, liver, and pancreas of Sprague Dawley rats. The findings included tissue perforation in all three organs, endothelial nuclear damage in the kidney, central vein wall damage in the liver, and cellular disintegration in the pancreatic islets of Langerhans. Based on these findings, it can be concluded that a fixation duration of three weeks does not produce optimal histological images and therefore cannot be used as a reference for establishing a standard histotechnology SOP in the laboratory of the Faculty of Medicine, Syarif Hidayatullah State Islamic University Jakarta.

Induksi Metastasis Limfonodus oleh DMBA Topikal pada Model Mencit C3H/HeJ dengan Karsinoma Sel Skuamosa Fitria Tahta Alfina; Devy Ariany; Nurlaely Mida Rachmawati; Luluk Hermawati; Ghina Mutiara Abas
Jurnal Ilmiah Kedokteran dan Kesehatan Vol. 5 No. 2 (2026): Mei: Jurnal Ilmiah Kedokteran dan Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/klinik.v5i2.6491

7,12-Dimethylbenz(a)anthracene (DMBA) is an environmental carcinogen. Topical application of DMBA in mice induces skin tumors with 100% incidence, and approximately 40% progress to squamous cell carcinoma (SCC), which carries a high risk of metastasis. To evaluate lymph node metastasis in SCC following topical administration of 0.125% DMBA. This research was conducted as a laboratory experimental study A total of 200 µl of 0.125% DMBA was applied topically to the dorsal skin of C3H/HeJ mice three times a week for 10 weeks. All mice with SCC showed metastatic deposits in the lymph nodes, characterized by anaplastic cells with hyperchromatic nuclei and a 1:1 nucleus-to-cytoplasm ratio. Significant differences in follicular area were found between the malignant DMBA group and the vehicle (mean difference 0.26; P<0.001), distilled water (0.25; P<0.001), and between the benign DMBA group and both vehicle (0.28; P<0.001) and distilled water (0.27; P < 0.001). No significant differences were observed between the malignant and benign DMBA groups (P = 0.767), or between vehicle and distilled water (P = 0.884). Topical application of 0.125% DMBA for 10 weeks is associated with lymph node metastasis in SCC, which can be identified through histological observation of tumor cell deposits and follicular hyperplasia in lymph nodes.

Effect of Kiwi Fruit (Actinidia deliciosa) Extract on Histological Structure and Testicular Weight in Monosodium Glutamate-Induced Male Rats Desti Asihanti Saputra; Devy Ariany; Lucky Brilliantina; Luluk Hermawati; Kamalia Layal
International Journal of Medicine and Health Vol. 5 No. 3 (2026): September: International Journal of Medicine and Health
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/ijmh.v5i3.6384

Monosodium glutamate (MSG) is a widely consumed food additive that has been reported to increase the production of reactive oxygen species (ROS), potentially inducing damage to various organs, including the testes. Oxidative stress in the testes may impair histological structure and disrupt the process of spermatogenesis. Antioxidants have been shown to attenuate the harmful effects of free radicals, and kiwifruit (Actinidia deliciosa) is recognized as a rich source of natural antioxidants. This study aimed to evaluate the effect of kiwifruit extract on testicular weight and histopathological characteristics in male Sprague Dawley rats exposed to MSG for 30 days. This experimental study used 25 male Sprague Dawley rats randomly assigned into five groups (n = 5 per group): negative control (K−), positive control receiving MSG at 4.8 g/kg body weight/day (K+), MSG plus vitamin C at 0.2 g/kg body weight/day (P1), MSG plus kiwifruit extract at 0.2 g/kg body weight/day (P2), and MSG plus kiwifruit extract at 0.4 g/kg body weight/day (P3). All treatments were administered for 30 consecutive days. Histopathological evaluation of the testes was performed on hematoxylin and eosin (H&E)-stained sections to assess the seminiferous tubule germinal epithelium, while testicular weight was measured at the end of the experiment. Data were presented descriptively as mean ± standard deviation. Histopathological examination demonstrated improved seminiferous tubule germinal epithelium in the P2 and P3 groups compared with the MSG-treated group (K+). The lowest mean testicular weight was observed in the P3 group (0.66 ± 0.31 g), compared with the K+ group (1.07 ± 0.28 g) and the negative control group (0.95 ± 0.30 g). Administration of kiwifruit extract tended to improve the histopathological appearance of the testes in male rats exposed to MSG, particularly in the P3 group, suggesting a potential protective effect against oxidative stress-induced testicular damage. However, the findings regarding changes in testicular weight remain descriptive and require confirmation through inferential statistical analysis.

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