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Preliminary Study in Overview of CYP2A6 Gene Variation in Infertile Male Patients Jihadin Rasyadi Mumtaz; Zeti Harriyati; Chris Adhiyanto; Nurul Hiedayati; Yona Mimanda; Muniroh Muniroh
The Avicenna Medical Journal Vol 2, No 1 (2021)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Introduction: Infertility is a condition which is described by WHO as inability for having children after one year of relationship without birth control. this condition can be caused both from inside and outside the body. One of the major outside factors is exposure to chemical substances, which in this case nicotine substance that came from cigarettes. The CYP2A6 gene is a coding gene for the xenobiotic metabolic enzyme, in this case nicotine which can cause infertility in men. Mutations in this gene can lead to nicotine buildup, increasing nicotine effect thus increasing the risk of infertility in men. The aim of this study was to determine whether there were variations in the CYP2A6 gene in male infertile patients.Methods: The method used was the isolation of infertile and fertile sperm DNA samples (five samples each) which were then amplified at the target DNA (CYP2A6) by PCR method and then sequenced to see the nucleutide sequence.Result: The results of this study showed that there were variations in the CYP2A6 gene that occurred only in infertile patients, heterogeneous mutations in the 7788th(C-A), 8040th (C-T), 7661st (G-C) nucleutide sequence of the CYP2A6 gene and SNP mutations in the 8250th(T>C), 8409th (T>G/C), and 8428th(C>T) nucleutide sequence of the CYP2A6 gene.Conclusion: The conclusion of this study is that there are CYP2A6 variations that found only in infertile male patients.

The Effect of Ramadan Fasting on Serum Malondialdehyde Levels as Indicator of Oxidative Stress and Cellular Damage Annisa Nadia Utami; Risahmawati . .; Muniroh . .
The Avicenna Medical Journal Vol 3, No 1 (2022)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v3i1.19716

Background: Ramadan fasting is one of five pillars of Islam. Fasting is to refrain from eating and drinking from sunrise (sahur) to sunset (ifthar). Ramadan fasting has been shown to impact on body systems in different manners. One of the benefits of Ramadan fasting is protection against oxidative stress and cellular damage. Malondialdehyde (MDA) is a product of free radicals (hydroxil radicals) with polyunsaturated fatty acids (PUFAs) produced from lipid peroxidation of cell membranes. This paper aims to determine the effect of Ramadan fasting on serum MDA levels.Methods: The design of this study is quasiexperimental with one group pre and post design. The sample of this study was 16 preclinical students of FK UIN Syarif Hidayatullah Jakarta who performed Ramadan fasting for 17 consecutive days. Blood samples were taken 1 day before Ramadan fasting and 18 days of Ramadan fasting to measure serum MDA levels. The study was conducted in the biochemical laboratory of FK UIN Syarif Hidayatullah.Results: The mean serum MDA levels before fasting was 0.90 ± 0.54 nmol/mL. The median value of serum MDA levels after fasting was 0.43 nmol/mL, with a range between 0.03 - 2.75 nmol/mL. Ramadan fasting decreases serum MDA levels, but the decrease that occurs on the 17th day of Ramadan fasting was not significant (p> 0.05). There was an increase in serum MDA levels in 4 subjects (25%) after Ramadan fasting.Conclusion: Ramadan fasting can reduce serum MDA levels as a marker of oxidative stress and cell damage. Further study is needed to investigate the various results in serum MDA levels after fasting.

Effect of Kelor Leaves (Moringa oleifera) in Liver Function of Streptozotocin induced Diabetic Sprague dawley Strain Male Rats Nashih Abdillah; Devy Ariany; Muniroh . Muniroh
The Avicenna Medical Journal Vol 3, No 2 (2022)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v3i2.27055

Background: Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia. Prolonged hyperglycemia can damage various organs of the body including the liver. Moringa oleifera leaves extract contain flavonoids which can decrease blood glucose levels and act as a hepatoprotector. To evaluate its hepatoprotective activity, liver function marker Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvate transminase (SGPT ) was used. In this study, we aimed to determine the effect of Moringa oleifera leaves extract on the liver function test of male Sprague dawley rats that have DM due to streptozotocin induction.Methods: This was experimental study conducted from February 2019 to July 2019 at Faculty of Medicine Laboratory, UIN Syarif Hidayatullah, Jakarta. This study conducted in 25 male Sprague dawley rats divided into 5 groups, those were negative controls group, positive controls group, and 3 treatment groups which given oral Moringa oleifera leaves extract at doses of 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW, respectively, for 14 days. Samples were collected on day 26th to measure SGOT and SGPT. Blood glucose levels were assayed on day 6th and 26th.Results: There was no significant decrease in SGOT (p=0.069) and SGPT (p=0.345) levels in the administration of Moringa oleifera leaves extract to male Sprague dawley rats with DM induced by streptozotocin at a dose of 200 mg/KgBW and 400 mg/KgBW.Conclusions: Moringa oleifera leaves extract did not significantly decrease SGOT and SGPT.

Effect of Kelor Leaves (Moringa oleifera) in Liver Function of Streptozotocin induced Diabetic Sprague dawley Strain Male Rats Nashih Abdillah; Devy Ariany; Muniroh . Muniroh
The Avicenna Medical Journal Vol 3, No 2 (2022)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v3i2.27055

Background: Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia. Prolonged hyperglycemia can damage various organs of the body including the liver. Moringa oleifera leaves extract contain flavonoids which can decrease blood glucose levels and act as a hepatoprotector. To evaluate its hepatoprotective activity, liver function marker Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvate transminase (SGPT ) was used. In this study, we aimed to determine the effect of Moringa oleifera leaves extract on the liver function test of male Sprague dawley rats that have DM due to streptozotocin induction.Methods: This was experimental study conducted from February 2019 to July 2019 at Faculty of Medicine Laboratory, UIN Syarif Hidayatullah, Jakarta. This study conducted in 25 male Sprague dawley rats divided into 5 groups, those were negative controls group, positive controls group, and 3 treatment groups which given oral Moringa oleifera leaves extract at doses of 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW, respectively, for 14 days. Samples were collected on day 26th to measure SGOT and SGPT. Blood glucose levels were assayed on day 6th and 26th.Results: There was no significant decrease in SGOT (p=0.069) and SGPT (p=0.345) levels in the administration of Moringa oleifera leaves extract to male Sprague dawley rats with DM induced by streptozotocin at a dose of 200 mg/KgBW and 400 mg/KgBW.Conclusions: Moringa oleifera leaves extract did not significantly decrease SGOT and SGPT.

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