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All Journal Bioscientist : Jurnal Ilmiah Biologi Media Abdimas
Rosa D. Pangaribuan
Universitas Musamus
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Public Health Social Sciences Other

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Peningkatan Kesejahteraan Masyarakat Melalui Pelatihan Budidaya Pembesaran Ikan Lele dengan Sistem Bioflok Di Kampung Kuprik Kabupaten Merauke Lindon R. Pane; Jeremias Tuhumena; Dandy Saleky; Rosa D. Pangaribuan; Sendy L. Merly; Silas T. Redu; Andri Irawan; Rachmat Agung
Media Abdimas Vol 2 No 1 (2023): Jurnal Media Abdimas Vol 2 No 1 Bulan Maret 2023
Publisher : Universitas Persada Indonesia YAI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1416.786 KB) | DOI: 10.37817/mediaabdimas.v2i1.2669

Abstract

Pemeliharaan Ikan Lele pada bak terpal biasanya dilakukan pada lahan sempit danketersediaan air yang terbatas. Pada keadaan tersebut perlu dilakukan penyediaan air yangdapat mendukung usaha budidaya Ikan Lele, untuk menyediakan kualitas air yang baik perludilakukan pengelolaan air. Salah satu cara yang dilakukan untuk menyediakan air dengankualitas baik adalah dengan menggunakan sistem bioflok. Tujuan dari pengabdian ini yaitumelatih masyarakat pembudidaya ikan lele agar dapat lebih maksimal memahami bagaimanacara kerja dari sistem bioflok ini. Sistem ini menjadi salah satu alternatif pemecah masalahterlebih khusus daerah di Kabupaten Merauke. Dimana sumber air bersih yang sangat terbatas,memaksa para pembudidaya menambah pengeluaran dalam hal membeli air bersih.
DNA Barcoding and Phylogenetic Analysis of the Gastropod Terebralia semistriata from the Coastal Waters of Merauke, South Papua Province Dandi Saleky; Ferdinand C. Situmorang; Rosa D. Pangaribuan
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 3 (2026): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i3.20895

Abstract

This study aimed to identify the gastropod species Terebralia sp. from the coastal waters of Merauke, South Papua Province, using a molecular identification approach. The Cytochrome Oxidase Subunit I (COI) gene was amplified through the Polymerase Chain Reaction (PCR) method using the forward primer LCO-1490 (5′-GGTCAACAAATCATAAAAGATATTGG-3′) and the reverse primer HCO-2198 (5′-TAAACTTCAGGGTGACCAAAAATCA-3′). DNA sequence analyses were performed using MEGA X software. A total of five samples were successfully amplified, producing DNA fragments with a length of 658 bp. Identification based on the DNA barcoding approach confirmed that all analyzed specimens belonged to Terebralia semistriata, with sequence similarity values ranging from 98.94% to 100% among samples. The genetic distance values obtained ranged from 0.003 to 0.012. Phylogenetic reconstruction using the Neighbor-Joining method with the Kimura 2-Parameter model revealed clustering patterns of sequences based on genetic similarity and interspecific genetic distances. Molecular identification using DNA barcoding provides an effective approach for accurate species identification and supports sustainable management and utilization of gastropod resources.
Co-Authors Andri Irawan Dandi Saleky Dandy Saleky Ferdinand C. Situmorang Jeremias Tuhumena Lindon R Pane Rachmat Agung Sendy L. Merly Silas Tanggu Redu