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All Journal Biopropal Industri ANNALES BOGORIENSES Annales Bogorienses
Nuriyanah Nuriyanah, Nuriyanah
Lembaga Ilmu Pengetahuan Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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POTENSI BAKTERI ENDOFITIK DARI TANAMAN KELADI TIKUS SEBAGAI PENGHASIL ZAT ANTIMIKROBA DAN ANTIOKSIDAN Sukiman, Harmastini; Nuriyanah, Nuriyanah
Biopropal Industri Vol 7, No 1 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3143.476 KB)

Abstract

Typhonium flagelliforme (Lodd.) known as a medicinal plant to cure generative disease such as cancer. This research is focusing on isolation of endophytes bacteria from Typhonium flagelliforme (Lodd.). Twenty six of endophytes bacteria have been successfully isolated. Results indicated that nine isolates of bacteria could inhibit the growth of Bacillus subtilis and three isolates could inhibit Staphylococus aureus. The strongest secretion showed by KTD4 was 3.029 for Bacillus subtilis and KTBt1 2.042 for Staphylococcus aureus. Antioxidant activity tests of four selected isolates showed that KTBt4 could produce highest inhibition percentage up to 74.68% and IC50 68.103 ppm compared to vitamin C which is just 3.053 ppm.Keywords: antioxidant, endophyte, Typhonium flagelliforme (Lodd.) ABSTRAKKeladi tikus atau Typhonium flagelliforme (Lodd.) dikenal sebagai tanaman obat yang dapat menyembuhkan berbagai penyakit, khususnya kanker. Penelitian ini difokuskan untuk mengisolasi bakteri endofitik tanaman keladi tikus. Sebanyak 26 isolat bakteri berhasil diisolasi. Hasil uji menunjukkan bahwa 9 isolat bakteri dapat menghambat pertumbuhan Bacillus subtilis dan 3 isolat terhadap Staphylococcus aures. Kekuatan sekresi tertinggi dihasilkan oleh isolat KTD4 (bagian daun) yang mencapai 3,029 untuk Bacillus subtilis dan KTBt1 (bagian batang) 2,042 untuk Staphylococcus aureus. Uji aktivitas antioksidan dilakukan terhadap empat isolat terpilih menunjukkan bahwa isolat KTBt4 (bagian batang) memberikan presentasi nilai inhibisi tertinggi yakni 74,68% dan nilai IC50 68,103 bpj dibandingkan dengan vitamin C yang mencapai 3,053 bpj.Kata kunci: antioksidan, endofit, keladi tikus
Potency of Endophyte Bacterium Isolated from Shorea selanica on Producing IAA Hormone and Supporting the Growth of Soybean Widowati, Tiwit; Nuriyanah, Nuriyanah; Sukiman, Harmastini
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.079 KB) | DOI: 10.1234/79

Abstract

Growth of soybean plants was supported by the presence of nitrogen fixing bacteria. Besides nitrogen, other elements such as phosphate, potassium and growth hormones are also required. Endophytic bacteria associated with Shorea selanica were isolated and tested for their ability to produce indole acetic acid (IAA) hormone and exhibit stress tolerance. Colorimetric analysis showed that isolate SSBt2 produced the highest IAA (43.01 µg/ml) in culture supplemented with L-tryptophan. Isolate SSBt2 grew well in the some stress tests, except on heat and oxidative stress. The highest IAA producing strain was selected for determining its capability and compatibility to support the growth of soybean plants in glass house experiment. The results indicated that the endophytic bacteria isolated from S. selanica are compatible to support the growth of soybean. SSBt2 was identified as Enterobacter hormaechei based on 16S rRNA gene analysis.
Potency of Endophyte Bacterium Isolated from Shorea selanica on Producing IAA Hormone and Supporting the Growth of Soybean Widowati, Tiwit; Nuriyanah, Nuriyanah; Sukiman, Harmastini
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
Publisher : BRIN

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Abstract

Growth of soybean plants was supported by the presence of nitrogen fixing bacteria. Besides nitrogen, other elements such as phosphate, potassium and growth hormones are also required. Endophytic bacteria associated with Shorea selanica were isolated and tested for their ability to produce indole acetic acid (IAA) hormone and exhibit stress tolerance. Colorimetric analysis showed that isolate SSBt2 produced the highest IAA (43.01 μg/ml) in culture supplemented with L-tryptophan. Isolate SSBt2 grew well in the some stress tests, except on heat and oxidative stress. The highest IAA producing strain was selected for determining its capability and compatibility to support the growth of soybean plants in glass house experiment. The results indicated that the endophytic bacteria isolated from S. selanica are compatible to support the growth of soybean. SSBt2 was identified as Enterobacter hormaechei based on 16S rRNA gene analysis.
Co-Authors Harmastini Sukiman, Harmastini Tiwit Widowati, Tiwit