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ISOLASI DAN IDENTIFIKASI KAPANG ENDOFIT DARI TANAMAN KUNYIT (Curcuma longa L.) SEBAGAI PENGHASIL ANTIOKSIDAN Widowati, Tiwit; Bustanussalam, Bustanussalam; Sukiman, Harmastini; Simanjuntak, Partomuan
Biopropal Industri Vol 7, No 1 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Endophyte fungi are microbe that living inside the plant tissue without harming the host plant. Endophyte fungi can produce secondary metabolite which can be used as antioxidant, anticancer and antimicobes compound. Endophyte fungi can be found in many plants especially herbs such as turmeric (Curcuma longa L). The aims of this study are to isolate and identify endophyte fungi from stem of C. longa L. which is potential as an antioxidant producer. The endophyte fungi isolated from turmeric stem were 12 isolates. Antioxidant activity was assayed using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) showed that isolate K.Cl.Sb.B1 produced the highest inhibition value (78,81%). Based on molecular identification, the isolate K.Cl.Sb.B1 was Colletotrichum sp.Keywords: Curcuma longa L., endophyte fungi, identification antioxidantÂ ABSTRAKKapang endofit merupakan mikroba yang terdapat di dalam jaringan tanaman tanpa membahayakan tanaman inang. Kapang endofit mampu menghasilkan metabolit sekunder yang dapat dimanfaatkan sebagai senyawa antioksidan, antikanker dan antimikroba. Kapang endofit dapat ditemukan pada berbagai jenis tanaman terutama tanaman obat seperti kunyit (Curcuma longa L). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang endofit dari batang tanaman kunyit yang berpotensi sebagai penghasil antioksidan. Kapang endofit yang diisolasi dari batang tanaman kunyit diperoleh 12 isolat. Uji antioksidan menggunakan 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) menunjukkan bahwa isolat K.Cl.Sb.B1 menghasilkan nilai inhibisi tertinggi (78,81%). Berdasarkan identifikasi molekuler, isolat K.Cl.Sb.B1 merupakan Colletotrichum sp.Kata kunci: Curcuma longa L., identifikasi antioksidan, kapang endofit

POTENSI BAKTERI ENDOFITIK DARI TANAMAN KELADI TIKUS SEBAGAI PENGHASIL ZAT ANTIMIKROBA DAN ANTIOKSIDAN Sukiman, Harmastini; Nuriyanah, Nuriyanah
Biopropal Industri Vol 7, No 1 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Typhonium flagelliforme (Lodd.) known as a medicinal plant to cure generative disease such as cancer. This research is focusing on isolation of endophytes bacteria from Typhonium flagelliforme (Lodd.). Twenty six of endophytes bacteria have been successfully isolated. Results indicated that nine isolates of bacteria could inhibit the growth of Bacillus subtilis and three isolates could inhibit Staphylococus aureus. The strongest secretion showed by KTD4 was 3.029 for Bacillus subtilis and KTBt1 2.042 for Staphylococcus aureus. Antioxidant activity tests of four selected isolates showed that KTBt4 could produce highest inhibition percentage up to 74.68% and IC50 68.103 ppm compared to vitamin C which is just 3.053 ppm.Keywords: antioxidant, endophyte, Typhonium flagelliforme (Lodd.)Â ABSTRAKKeladi tikus atau Typhonium flagelliforme (Lodd.) dikenal sebagai tanaman obat yang dapat menyembuhkan berbagai penyakit, khususnya kanker. Penelitian ini difokuskan untuk mengisolasi bakteri endofitik tanaman keladi tikus. Sebanyak 26 isolat bakteri berhasil diisolasi. Hasil uji menunjukkan bahwa 9 isolat bakteri dapat menghambat pertumbuhan Bacillus subtilis dan 3 isolat terhadap Staphylococcus aures. Kekuatan sekresi tertinggi dihasilkan oleh isolat KTD4 (bagian daun) yang mencapai 3,029 untuk Bacillus subtilis dan KTBt1 (bagian batang) 2,042 untuk Staphylococcus aureus. Uji aktivitas antioksidan dilakukan terhadap empat isolat terpilih menunjukkan bahwa isolat KTBt4 (bagian batang) memberikan presentasi nilai inhibisi tertinggi yakni 74,68% dan nilai IC50 68,103 bpj dibandingkan dengan vitamin C yang mencapai 3,053 bpj.Kata kunci: antioksidan, endofit, keladi tikus

The Effect of Bio-VA Mycorrhizae Inoculation on Biomass of Trees and Carbon Stock of Eight Forest Tree Species in Bodogol Plantation-Sukabumi, Indonesia Sukiman, Harmastini; Heriyanto, NM.
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2309

Studi tentang pengaruh Bio-Mycorrhizae (BIO-VAM) pada biomasa dari delapan species tanaman dan stok karbonnyadilakukan dengan metoda destruksi sampel dari tanaman yang terpilih di Bodogol, Sukabumi, Jawa Barat. Data yangdidapat dari penelitian menunjukkan bahwa inokulasi Bio-VAM menunjukkan hasil yang positif terhadap biomasatanaman dan stok karbon. Volume biomasa tanaman tertinggi ditunjukkan oleh species tanaman umbrella tree (Maesopsisemenii. Eng) yakni 387,5 g per tanaman apabila diinokulasi dengan mikorisa dan 211,6 g per tanaman tanpa mikorisadiikuti dengan sengon buto (Enterolobium cyclocarpa Griseb), rasamala (Altingia excelsa Noronhae), manglid (Magnoliablumei Prantl), sawo duren (Chrysophyllum cainito L)., ki bereum (Adinandra dumosa Jack) bisbul (Diospyros blancoiDesr.) dan biomasa tanaman terendah ditunjukkan oleh species podocarp/ jamuju (Podocarpus imbricata R.Br) 82,76 gdengan inokulasi mikorisa dan 65,77 g tanpa inokulasi mikorisa. Karbon stok tertinggi ditunjukkan oleh tiga spesiestanaman yakni umbrella tree (Maesopsis emenii. Eng.) 3,23 ton C/ha dengan inokulasi mikorisa sementara tanaman kontrolmenunjukkan 1,76 ton C /ha, dan 11,85 ton CO2 /ha dan 6,46 CO2 /ha. Diikuti oleh sengon buto (Enterolobium cyclocarpaGriseb), rasamala (Altingia excelsa Noronha) Kontribusi karbon stok terendah ditunjukkan oleh podocarp (Podocarpusimbricata Blume) yakni 0,07 ton C/ha (dengan inokulasi BioVAM ) dan 0.05 ton C/ha (tanpa inokulasi BioVam) 0,26ton CO2 /ha dan 0,18 ton CO2. Tujuan dari penelitian ini adalah menganalisa pengaruh inokulasi Bio-VA Mycorrhizaeterhadap produksi biomasa tanaman dan stok karbon selama pertumbuhan tanaman.Kata Kunci: BIO-VAMycorrhizae, inokulasi, biomas tanaman hutan , stok karbon.

ENDOFIT Taxus sumatrana (Miquel) de Laubenfels DAN POTENSINYA DALAM MEMPRODUKSI SENYAWA BIOAKTIF SEBAGAI SUMBER ANTIOKSIDAN Sukiman, Harmastini
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.750

Taxus sumatrana (Miquel) de Laubenfels is known as an endanger forest tree species grown at Kerinci National Park in Jambi,Indonesia. This plant was known potential on producing taxol. Taxol is a bioactive compound, could be used as antibacterial agent and recently confirmed to cure cancer cells. For the first time in 1960, Arthur Barclay found the taxol compound from Taxus sp.(pacific yew). However to isolate the bioactive compound, huge amount of tree biomass is needed. Research on endophytes microbes which are isolated from inner tissue of Taxus sp. declared that those microbes have potential on producing antioxidant agent for drug discovery. Isolation and conservation of endophytes and selecting its potential is promising the novel of finding new drug that may be effective for treating the newly developing diseases in human. Fifteen isolates of Taxus endophytes have been successfully studied on their ability on producing antioxidant. The results showed that endophytes fungus isolates TsC-17, isolated from Taxus sumatrana grown in Cibodas Botanical Gardens-LIPI, could produce extracellular bioactive compound which performed activity of suppressing free radical, is significantly better compared to intracellular bioactive compound eventhough it is not as high as in vitamin C. The activity of suppressing free radical resulted from bioactive compound of endophyte fungus isolates TsC-17 was 20% for intracellular bioactive compound, while it was 60% for extracellular bioactive compound and 90% for vitamin C.

RESPON TANAMAN PADI GOGO (Oryza sativa L.) TERHADAP STRESS AIR DAN INOKULASI MIKORISA Sukiman, Harmastini; Adiwirman, Adiwirman; Syamsiyah, Syofiatin
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

It has been known that upland rice production is lower than low land rice production because it is inhibited by soil fertility, water supply and pest.One solutions to increase the production of upland rice is by applying the potential soil microorganism known as mycorrhizae.The objective of this research is to know thef Vesicular Arbuscular Mycorrhizae (VAM) role for upland rice under water stress condition. The research was conducted in greenhouse of Cikabayan, Bogor Agricultural University and Soil Microbiology Laboratory of Research Centre of Biotechnology, Indonesian Institute of Science Centre. The experimental design was factorial randomized block design with two factors. The first factor is inoculation of mycorrhizae consisted of two treatment i.e. without mycorrhizae (MO) and with mycorrhizae (Ml); while the second factor is water stress regime consisted of five treatments namely well watered (SO), water stress on tillering stage (SI), primordial stage (S2), anthesis stage(S3) and grain filling stage(S4). Mycorrhizae significantly increased root infection, phosphate uptake, height, number of tillering, leaf, Leaf Area Index (LAI), productive tillering, filled grain, grain weight and yield. While, water stress significantly decreased number of root infection, shoot wet fresh weight, root dry weight, increased phosphate uptake and sterilized grain, but it didnt significantly decrease yield. The interaction both mycorrhizae and water stress didnt significantly influence all variables, except the root dry weight. The inoculation of mycorrhizae could increase 19.62% on SI, 17.32% on S2, 29.14% on S3, 6.89% on S4 dried harvested yield.

MIKROBAENDOFITIK DARI TAMAN NASIONALBATANG GADIS SUMATERA UTARA: POTENSINYA DALAM MENGHASILKAN SENYAWA ANTIMIKROBATERHADAPMIKROBAPATOGEN Sukiman, Harmastini; Lekatompessy, Sylvia; Widowati, Tiwit
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Batang Gadis National Park (TNBG) is one of the tropical forests in North Sumatra with megabiodiversity of flora and fauna including microorganism. Endophytic microbes conservation from varieties of forest plants in Batang Gadis National Park has been completed. Nineteen endophytes isolates from TNBG have been screened for their potential on producing bioactive compound against the pathogenic bacteria. Qualitative screening has been done using the growth agar media and identified the clear zone appeared surrounding the bacteria colony. The result indicated that isolate MSCI 87.4 showed high strengthening secresion (4.35) againts Xanthomonas campestris, whereas isolate MSCI 37.1 showed high secretion against Bacillus subtilis (2.69) and Escherichia coli (2.60). Isolate MSCI 37.4 showed potential on producing bioactive compound againts Staphylococcus aureus (4.41). Isolates MSCI 87.4, MSCI37.1, MSCI 37.4 and MSCI 58.1 even could produce bioactive compound against four pathogenic bacteria that are Xanthomonas campestris, Bacilus subtilis, Escherichia coli and Staphylococcus aureus, whereas isolate MSCI 15.5b potential on producing bioactive compound against two isolates namely Xanthomonas campestris and Bacillus subtilis. The endophytes bacteria mainly belong to the Gram negative group and four out of nineteen isolates tested belong to the Gram positive group. The cell mainly coccus and only one is bacilli without fiagella. The five most potential isolates has been maintained under freeze dried condition for futher conservation and study. Thin Layer Analysis using semipolar organic separation solution showed that most isolates identified were able to produce bioactive compound except two isolates i.e. MSCI 48.4a and MSCI 53.1; however further analysis is needed to confirm the product.

Potency of Endophyte Bacterium Isolated from Shorea selanica on Producing IAA Hormone and Supporting the Growth of Soybean Widowati, Tiwit; Nuriyanah, Nuriyanah; Sukiman, Harmastini
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Growth of soybean plants was supported by the presence of nitrogen fixing bacteria. Besides nitrogen, other elements such as phosphate, potassium and growth hormones are also required. Endophytic bacteria associated with Shorea selanica were isolated and tested for their ability to produce indole acetic acid (IAA) hormone and exhibit stress tolerance. Colorimetric analysis showed that isolate SSBt2 produced the highest IAA (43.01 Âµg/ml) in culture supplemented with L-tryptophan. Isolate SSBt2 grew well in the some stress tests, except on heat and oxidative stress. The highest IAA producing strain was selected for determining its capability and compatibility to support the growth of soybean plants in glass house experiment. The results indicated that the endophytic bacteria isolated from S. selanica are compatible to support the growth of soybean. SSBt2 was identified as Enterobacter hormaechei based on 16S rRNA gene analysis.

ENDOPHYTES RHIZOBIA BACTERIA, ISOLATED FROM ACACIA MANGIUM, ITS POTENSIAL TO SUPPORT THE GROWTH OF SOYBEAN. Sukiman, Harmastini
Teknologi Indonesia Vol 38, No 3 (2015)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v38i3.225

Acacia mangium is a legume tree species which has high tolerance with many different conditions and dry area. Aim of this research is to study the possibility of cross inoculation of rhizobium endophytic bacteria isolated from Acacia mangium to soybean plant and to find out the best combination of inoculants to develop biofertilizers quality. Isolation of rhizobium endophytes from Acacia mangium analysed using 16S rRNA methods identified that the bacteria belonged to Rhizobia tropici. Results showed that single inoculation of endophytic bacteria can produce soybean seed up to 19.71 gram which was slightly higher compared with control plants of 16.0 gram and chemical fertilizer treatment plants of 18.0 gram per plant. Meanwhile, Rhizobium inoculation still gave the best production of soybean; that is 28 gram per plant. Moreover, stress test results showed that the bacteria could adapt better with acidity and salinity, including UV exposure and cold condition, yet were unable to adapt with hightemperature and oxidative condition.

THE EFFECT OF BIO-VA MYCORRHIZAE INOCULATION ON BIOMASS OF TREES AND CARBON STOCK OF EIGHT FOREST TREE SPECIES IN BODOGOL PLANTATION-SUKABUMI, INDONESIA Sukiman, Harmastini; Heriyanto, NM.
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2309

Studi tentang pengaruh Bio-Mycorrhizae (BIO-VAM) pada biomasa dari delapan species tanaman dan stok karbonnyadilakukan dengan metoda destruksi sampel dari tanaman yang terpilih di Bodogol, Sukabumi, Jawa Barat. Data yangdidapat dari penelitian menunjukkan bahwa inokulasi Bio-VAM menunjukkan hasil yang positif terhadap biomasatanaman dan stok karbon. Volume biomasa tanaman tertinggi ditunjukkan oleh species tanaman umbrella tree (Maesopsisemenii. Eng) yakni 387,5 g per tanaman apabila diinokulasi dengan mikorisa dan 211,6 g per tanaman tanpa mikorisadiikuti dengan sengon buto (Enterolobium cyclocarpa Griseb), rasamala (Altingia excelsa Noronhae), manglid (Magnoliablumei Prantl), sawo duren (Chrysophyllum cainito L)., ki bereum (Adinandra dumosa Jack) bisbul (Diospyros blancoiDesr.) dan biomasa tanaman terendah ditunjukkan oleh species podocarp/ jamuju (Podocarpus imbricata R.Br) 82,76 gdengan inokulasi mikorisa dan 65,77 g tanpa inokulasi mikorisa. Karbon stok tertinggi ditunjukkan oleh tiga spesiestanaman yakni umbrella tree (Maesopsis emenii. Eng.) 3,23 ton C/ha dengan inokulasi mikorisa sementara tanaman kontrolmenunjukkan 1,76 ton C /ha, dan 11,85 ton CO2 /ha dan 6,46 CO2 /ha. Diikuti oleh sengon buto (Enterolobium cyclocarpaGriseb), rasamala (Altingia excelsa Noronha) Kontribusi karbon stok terendah ditunjukkan oleh podocarp (Podocarpusimbricata Blume) yakni 0,07 ton C/ha (dengan inokulasi BioVAM ) dan 0.05 ton C/ha (tanpa inokulasi BioVam) 0,26ton CO2 /ha dan 0,18 ton CO2. Tujuan dari penelitian ini adalah menganalisa pengaruh inokulasi Bio-VA Mycorrhizaeterhadap produksi biomasa tanaman dan stok karbon selama pertumbuhan tanaman.Kata Kunci: BIO-VAMycorrhizae, inokulasi, biomas tanaman hutan , stok karbon.

Potency of Endophyte Bacterium Isolated from Shorea selanica on Producing IAA Hormone and Supporting the Growth of Soybean Widowati, Tiwit; Nuriyanah, Nuriyanah; Sukiman, Harmastini
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Growth of soybean plants was supported by the presence of nitrogen fixing bacteria. Besides nitrogen, other elements such as phosphate, potassium and growth hormones are also required. Endophytic bacteria associated with Shorea selanica were isolated and tested for their ability to produce indole acetic acid (IAA) hormone and exhibit stress tolerance. Colorimetric analysis showed that isolate SSBt2 produced the highest IAA (43.01 μg/ml) in culture supplemented with L-tryptophan. Isolate SSBt2 grew well in the some stress tests, except on heat and oxidative stress. The highest IAA producing strain was selected for determining its capability and compatibility to support the growth of soybean plants in glass house experiment. The results indicated that the endophytic bacteria isolated from S. selanica are compatible to support the growth of soybean. SSBt2 was identified as Enterobacter hormaechei based on 16S rRNA gene analysis.

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