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All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Agrosilvopasture-Tech
Johanna Taribuka, Johanna
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Agriculture, Biological Sciences & Forestry

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EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.933 KB)

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.943 KB) | DOI: 10.23960/j.hptt.216115-123

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
Daya Hambat Jamur Endofitik Terhadap Fusarium oxysporum f.sp. capsici Penyabab Layu Fusarium Pada Tanaman Cabai (Capsicum frutescens L.) Polhaupessy, Vanda C; Taribuka, Johanna; Talahaturuson, Abraham
Jurnal Agrosilvopasture-Tech Vol 4 No 1 (2025): Jurnal Agrosilvopasture-Tech
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/j.agrosilvopasture-tech.2025.4.1.100

Abstract

This research aims to obtain endophytic fungi from healthy chili plants (Capsicum frutescens L.) and test the inhibitory power of endophytic fungi, which are thought to have the potential to control the pathogen that causes fusarium wilt disease (Fusarium oxysporum f.sp. capsici) in chili plants (Capsicum frutescens L.). The exploration results obtained five genera of endophytic fungi from different plant parts and in different villages and hamlets, including Colletotrichum sp.TWtc, Rhizoconia sp.TWdc, Trichoderma sp.TKac, Penicillium sp.TKbc, and Aspergilus sp.TObtc, The percentage of inhibitory power of the five endophytic fungal genera against the pathogenic fungus F.oxysporum f.sp capsici is as follows: Trichoderma sp.TKac, which is 85.29%, followed by the isolate Rhizoctonia sp.TWdc, which is 62.14%, isolates Penicillium sp. TKbc was 61.31%, Colletotrichum sp.TWtc isolate was 60.27% and the lowest inhibitory power was Aspergillus sp.TObtc isolates were 46.42%. Conclusion: The endophytic fungus Trichoderma sp. TKac has the highest inhibitory ability against the pathogenic fungus F.oxysporum f.sp.capsici, so it can be used as a biological control agent.
Co-Authors Abraham Talahaturuson, Abraham Arif Wibowo Christanti Sumardiyono Polhaupessy, Vanda C SITI MUSLIMAH WIDYASTUTI Yoga Yuniadi