Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

0.408

P-Index

This Author published in this journals

All Journal Jurnal Fitopatologi Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL AGROTEKNOLOGI Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Christanti Sumardiyono

Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281

Author-ID : 3094298

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Published : 35 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4

EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.933 KB)

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen FusariumÂ oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to TrichodermaÂ asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis,Â with the homologous value of 99%.

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

Karakterisasi Isolat Rhizoctonia sp. Patogenik dan Rhizoctonia Mikoriza Pada Tanaman Anggrek Tanah Spathoglottis plicata Soelistijono, Soelistijono; Priyatmojo, Achmadi; Semiarti, Endang; Sumardiyono, Christanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Rhizoctonia mikoriza merupakan jamur yang mampu berasosiasi dengan anggrek tanah. Selain sebagai mikoriza, terdapat isolat Rhizoctonia sp. patogen dan penyebab penyakit busuk akar pada Spathoglottis plicata. Penelitian ini bertujuan mengetahui perbedaan antara Rhizoctonia sp. patogen dan Rhizoctonia mikoriza secara morfologi dan molekular menggunakan teknik RAPD. Hasil penelitian secara morfologi menunjukkan bahwa warna koloni, panjang dan jumlah inti sel isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza pada S. plicata tidak berbeda, tetapi berbeda pada ketebalan sel dan pengelompokan isolat berdasarkan uji anastomosis hifa. Teknik molekuler RAPD menunjukkan bahwa setiap isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza memiliki perbedaan pada struktur DNA.

Pengaruh Lengas Nisbi dan Suhu terhadap Kerusakan Gaplek Akibat Aspergillus flavus Selama Penyimpanan Oramahi, H. A.; Sumardiyono, Christanti; Pusposendjojo, Nursamsi; Haryadi, Haryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

A study on the spoilage of dried cassava by Aspergillus flavus was still limited so that such study is very important to do. This experiment was done to examine the effect of relative humidity (RH) and storage room temperature on the development of A. flavus and dried cassava spoilage during storage time. Based on the population of A. flavus, starch content, reduction sugar content, and water content of dried cassava, it was concluded that RH 65%, the temperature of 30 and 35oC had better storage condition for dried cassava because it resulted in the lowest population of A. flavus and the lowest deterioration of dried cassava.

Dominansi dan Seleksi Jamur Aspergillus Perusak Gaplek Oramahi, H. A.; Sumardiyono, Christanti; Pusposendjojo, Nursamsi; Haryadi, Haryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Dried cassava is a suitable host for the growth and development of the genus Aspergillus. Fungi reported to grow on dried cassava were A. clavatus, A. flavus, A. foetidus, A. fumigatus, A. niger, A. ochraceus, A. oryzae, A. tamarii, A. zonatus, dan Aspergillus sp. From those species, A. flavus is the most important species because of its toxigenic characteristic on agricultural product. This study was conducted to study dominant species of Aspergillus causing the most severe deterioration on stored dried cassava and causing the highest deterioration in dried cassava. The isolates were then cultured for determination of dominant species. The in vitro experiment was to obtain the species of Aspergillus that is resulted in highest change of dried cassava spoilage. Based on relative index frequency (Rif) and presence index of the fungus (Pif), A. flavus was the most dominant species and causing the highest deterioration on the dried cassava.

Pengendalian Penyakit Budok dengan Fungisida dan Deteksi Residu pada Daun Nilam Christanti Sumardiyono; Sedyo Hartono; Nasrun Nasrun; Sukamto Sukamto
Jurnal Fitopatologi Indonesia Vol 9 No 3 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Budok caused by Synchytrium pogostemonis is the important and destructive disease on patchouli plant (Pogostemon cablin). Chemical control must be done in incidence with high disease intensity and prediction of outbreak. The experiment was done using benomyl (Benlate 50 WP) and cuprous oxide (Kocide 77WP) 0.1%, 0.2% and mixture of both ( 1 g cuprous oxide and 1 g L-1 benomyl). Patchouli plant was sprayed eight times with one week interval. Harvesting was done two weeks after the end of spraying. Residue analysis of Cu was done with AAS and benomyl residue with HPLC. The result showed that 0.1% benomyl significantly reduced the disease intensity. The other treatments showed lower result. Harvested patchouli plants treated with 0.1% benomyl showed higher yield compare with 0.2% benomyl and cuprous oxyde. Cu residue in dried leaves was between 460 ppm and 950 ppm. Residue of benomyl was 54–100 ppb. Diseased seedling still can be used after spraying by benomyl four times with 2 weeks interval.

Ketahanan Beberapa Jamur Patogen terhadap Fungisida Christanti Sumardiyono; Nursamsi Pusposendjojo; Sri Trisnowati
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

Chemical control of plant pathogens have been done for a long time using contact and systemic fungicides. Resistance of the pathogens to fungicides may caused failure of disease control program. Studies by in vitro, in green house and fields were done at Yogyakarta to know the fungal which were resistant to several fungicides after treatment. The pathogen tested to fungicides were Colletotrichum capsici on red pepper to propineb, mancozeb, mixture of carbendazim and mancozeb, and thiophanate methyl, Alternaria porri on garlic to thiophanate methyl, Phytophthora palmivora on cocoa to Al-fosetyl, metalaxyl, mancozeb, and Copper Oxychloride, and Peronosclerospora maydis on corn to metalaxyl. The study indicated that C. capsici was resistant to propineb, mancozeb, and mixture of carbendazim and mancozeb, but sensitive to thiophanate methyl. A. porri from plant treated with thiophanate methyl indicated resistant to the fungicides. ED50 of Al-fosetyl and metalaxyl on P. palmivora were higher than on mancozeb and copper oxychloride, so that it was resistance strain.

Pengendalian Penyakit Karat Daun Kopi dengan Ekstrak Teh Hitam Christanti Sumardiyono; Setyo Agung
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

The research was done to know the efficacy of black tea extract to control coffee leaf rust, the most important disease of Arabica coffee caused by the fungus Hemileia vastatrix B et Br. Black tea was extracted by boiling 50 g of tea in 500 ml of water until 50 ml of solution was left and used as spraying material after 10 time dilution. Influence of black tea extract to urediospores germination was done by spore germination technique. Field experiment was done at Pagilaran Tea Plantation with a susceptible Arabica coffee Kate 26 variety. Coffee plants was sprayed with diluted tea extract eight times with 10 days interval and 0,3% of triadimefon was used as standart fungicide. The result showed that black tea extract inhibited germination of urediospora and reduced germtube length up to 10-4 dilution. The extract of black tea could reduce disease intensity of coffee leaf rust in the field better than triadimefon. Ten days after first spraying, disease intensity was reduced but it increased after 5 times spraying. After 8 times spraying disease intensity on coffee tree sprayed with the black tea extract was lower than that sprayed with tradimefon.

Karakterisasi dan Deteksi Cepat Bakteri Penyebab Penyakit Darah pada Pisang Nur Edy; Siti Subandiyah; Christanti Sumardiyono; Jaka Widada
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Blood disease of banana is one of the most serious banana disease in Indonesia. Although the disease has became the subject of quarantine it eventually spread and found in most provinces in Indonesia. The aim of this research were to identify the blood disease bacterium (BDB) using morphological observation, biochemical assay, pathogenicity testing of hosts range using infectivity titration and rapid detection by Polymerase Chain Reaction (PCR). The results showed that the blood disease bacterium could be differentiated from Ralstonia solanacearum race 2, the causal agent of Moko disease and R. solanacearum tobacco isolates. BDB isolates were not able to hydrolyze gelatin, Tween 80, starch, and were not able to produce nitrite from nitrate. They were only able to produce acid from galactose and glycerol. The pathogenicity test indicated that the BDB was only able to infect the banana/plantain and was not able to infect tomato, eggplant, and chili. Rapid detection using PCR method showed that the 121F/R primers was able to amplify the BDB genome and was not able to amplify the genome of R. solanacearum tobacco isolates.

Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium oxysporum f.sp. cubense) dengan Trichoderma sp. Albertus Sudirman; Christanti Sumardiyono; Siti Muslimah Widyastuti
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc) in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag), second factor was time of Trichoderma culture application (2 weeks before Foc inoculation, at same time with Foc inoculation and 2 weeks after Foc inoculation). Trichoderma sp. was cultured in mixed rice brand and chaff medium. The disease intensity was observed with scoring system of wilting leaves (0–4). The results showed that Trichoderma sp. was antagonistic against Foc in vitro and inhibited 86% of Foc colony development. Mechanism of antagonism between Trichoderma sp. and Foc was hyperparasitism. Trichoderma hyphae coiled around Foc hyphae. Lysis of Foc hyphae was occurred at the attached site of Trichoderma hyphae on Foc hyphae. Added banana seedling with Trichoderma sp. Culture reduced disease intensity of Fusarium wilt. Suggested dose of Trichoderma culture application in glass house was 25 g/polybag, given at the same time with Foc inoculation.

Co-Authors A.A. Ketut Agung Cahyawan W Achmadi Priyatmojo Ajri, Miftahul Albertus Sudirman Ani Widiastuti Ani Widiastuti Ani Widiastuti, Ani Aries Pratomo Arif Wibowo Arif Wibowo Arif Wibowo Arif Wibowo Arif Wibowo Bambang Hadisutrisno Christine Agustamia Didik Indradewa Dwi Kiswanti Edhi Martono Eko Agus Martanto Endang Semiarti Haryadi - Haryadi Haryadi Haryono Semangun Hasan Ashari Oramahi Ika Roostika Tambunan Iqna Khayatina Rusli Isam Eldeen Ibrahim Hassan Abdalla JAKA WIDADA Johanna Taribuka, Johanna Kardi Raharjo Liliek Sulistyowati Maemonah, Maemonah Nasrun Nasrun Niken Rasmi Paramita Nur Edy Nursamsi Pusposendjojo Nursamsi Pusposendjojo, Nursamsi Peter Taylor Putri Rositasari Santika, Islaminati Anna SEDYO HARTONO Setyo Agung Sismindari Sismindari SITI MUSLIMAH WIDYASTUTI Siti Muslimah Widyastuti Siti Subandiyah Siwi Indarti Sri Trisnowati Sudarmadi Sudarmadi Sukamto Sukamto Suryanti Suryanti Suryanti Suryanti Suryanti Tunjung Pamekas Wahyu Agustina Y. M.S. Maryudani Yoga Yuniadi Yufita Dwi Chinta Yufita Dwi Chinta Yuli Kristiawati

Title

Found 35 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 35 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 35 Documents
Search