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All Journal Plumula : Berkala Ilmiah Agroteknologi International Journal of Applied Biology Nusantara Science and Technology Proceedings
Endang Triwahyu Prasetyawati
Agrotechnology, Agriculture Faculty, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

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Formulation of Endophytic Bacteria in Organic Fertilizer as Health Promoter for Corn Plants in Mentaos, Gudo, Jombang Arika Purnawati; Tri Mujoko; Endang Triwahyu Prasetyawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3309

Abstract

Corn (Zea mays) has an important role in the food supply in Indonesia, as the second commodity after rice and the second source of carbohydrates after rice which is a derivative of rice and is still considered feasible to meet national needs. The increase in national corn production is strongly supported by several corn production center provinces such as East Java and one of the districts that have the potential to support corn production in East Java is Jombang, but the reality the production experiences several obstacles, one of which is the presence of plant pest organisms (OPT). namely the fungus Peronosclerospora maydis caused by downy mildew. In the control of disease so far in the field, many use fungicides that are harmful to humans and the environment. The solution to reduce the use of fungicides is to utilize endophytic bacteria from eggplant and it has been formulated with organic fertilizer so that it is easier to apply to corn plants, this solution is expected to control disease rates, reduce losses, and improve corn plant health. The method used in community service in Mentaos, Gudo, and Jombang were situation analysis, problem identification, socialization that was attended by 25 farmers, and application of endophytic bacteria from eggplant plants in organic fertilizer formulas. The results obtained are that the people of Mentaos, Gudo, and Jombang were able to apply the endophytic bacterial formula in organic fertilizer with the sprinkle technique. The results show that the community can apply the endophytic bacterial formula, corn plants become healthy, and disease intensity is low.
POTENSI KONSORSIUM Bacillus spp. DAN Pseudomonas fluorescens UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI RAWIT Nensi Agustina; Arika Purnawati; Endang Triwahyu Prasetyawati
Plumula : Berkala Ilmiah Agroteknologi Vol. 10 No. 1 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

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Abstract

Cabai rawit (Capsicum frutescens L.) merupakan salah satu tanaman hortikultura yang bernilai ekonomi tinggi. Produksi cabai rawit seringkali terkendala oleh penyakit layu batang yang diakibatkan cendawan Fusarium sp. Penelitian ini bertujuan untuk mengetahui pengaruh konsorsium bakteri Bacillus spp. dan Pseudomonas fluorescens sebagai agensia hayati pengendali penyakit layu fusarium pada tanaman cabai rawit. Penelitian ini dilaksanakan di screenhouse UPN “Veteran” Jawa Timur. Penelitian dimulai pada bulan Januari 2021 sampai bulan April 2021. Penelitian disusun menggunakan Rancangan Petak Terbagi (RPT) dengan dua faktor yaitu bakteri antagonis dan waktu aplikasi dan diulang sebanyak 4 kali. Faktor pertama yaitu bakteri antagonis dengan 5 taraf perlakuan yaitu Tanpa pemberian bakteri antagonis, Bacillus spp. kode isolat Ba9, Bacillus spp. kode isolat Ba17, P. fluorescens, Bacillus spp. kode isolat Ba9 + P. fluorescens, Bacillus spp. kode isolat Ba17 + P. fluorescens. Faktor kedua yaitu waktu aplikasi dengan 2 taraf perlakuan yaitu sebelum pindah tanam bibit dan setelah pindah tanam bibit. Jika diketahui adanya pengaruh yang nyata dari perlakuan maka dilakukan Uji Jarak Ganda Duncan atau Duncan Multiple Range Test (DMRT) pada taraf 5%. Perlakuan pemberian konsorisum Bacillus spp. kode isolat Ba9 dan P. fluorescens menyebabkan penundaan masa inkubasi Fusarium sp. sebesar 23,42%, meningkatkan tinggi tanaman sebesar 20,55 %, dan mempercepat munculnya calon bunga, sedangkan perlakuan pemberian konsorisum Bacillus spp. kode isolat Ba17 dan P. fluorescens menekan intensitas penyakit layu fusarium 29,03%, meningkatkan jumlah daun sebesar 56,58% dibandingkan dengan pemberian bakteri secara tunggal.
POTENSI BAKTERI Bacillus spp. DALAM MENGHAMBAT Colletotrichum capsici PENYEBAB ANTRAKNOSA PADA CABAI MERAH SECARA IN VITRO Sasiska Rani; Endang Triwahyu Prasetyawati; Herry Nirwanto
Plumula : Berkala Ilmiah Agroteknologi Vol. 10 No. 1 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

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Abstract

Colletotrichum capsici merupakan jamur patogen penting penyebab penyakit antraknosa pada cabai merah. Salah satu upaya untuk mengatasi permasalahan ini yaitu dengan penggunaan agens hayati bakteri Bacillus spp.. Penelitian ini bertujuan untuk mengetahui potensi lima isolat bakteri Bacillus spp. yaitu Ba-6, Ba-9, Ba-12, Ba-15, dan Ba-17 dalam menghambat patogen C. capsici Penelitian laboratorium dilakukan menggunakan Rancangan Acak Lengkap dengan 4 ulangan. Pengujian in vitro dilakukan dengan teknik dual culture pada media PDA dan detach fruit test pada buah cabai.Hasil penelitian menunjukkan efektifitas perhambatan C. capsici paling baik pada pengujian in vitro media PDA yaitu Bacillus sp. isolat Ba-9 dengan penghambatan sebesar 23,04%. Selanjutnya dalam uji detach fruit, Bacillus sp. isolat Ba-9 mampu menekan pertumbuhan C. capsici sebesar 21,25% dibandingkan kontrol.
Formulation of Endophytic Bacteria in Organic Fertilizer as Health Promoter for Corn Plants in Mentaos, Gudo, Jombang Arika Purnawati; Tri Mujoko; Endang Triwahyu Prasetyawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3309

Abstract

Corn (Zea mays) has an important role in the food supply in Indonesia, as the second commodity after rice and the second source of carbohydrates after rice which is a derivative of rice and is still considered feasible to meet national needs. The increase in national corn production is strongly supported by several corn production center provinces such as East Java and one of the districts that have the potential to support corn production in East Java is Jombang, but the reality the production experiences several obstacles, one of which is the presence of plant pest organisms (OPT). namely the fungus Peronosclerospora maydis caused by downy mildew. In the control of disease so far in the field, many use fungicides that are harmful to humans and the environment. The solution to reduce the use of fungicides is to utilize endophytic bacteria from eggplant and it has been formulated with organic fertilizer so that it is easier to apply to corn plants, this solution is expected to control disease rates, reduce losses, and improve corn plant health. The method used in community service in Mentaos, Gudo, and Jombang were situation analysis, problem identification, socialization that was attended by 25 farmers, and application of endophytic bacteria from eggplant plants in organic fertilizer formulas. The results obtained are that the people of Mentaos, Gudo, and Jombang were able to apply the endophytic bacterial formula in organic fertilizer with the sprinkle technique. The results show that the community can apply the endophytic bacterial formula, corn plants become healthy, and disease intensity is low.
The Potential of Bacillus spp. in Suppressing Colletotrichum capsici that Causes Antracnose in Red Chili Rani, Sasiska; Endang Triwahyu Prasetyawati; Herry Nirwanto; Sri Wiyatiningsih
International Journal of Applied Biology Vol. 7 No. 1 (2023): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Red Chili (Capsicum annum L.) is a horticultural plants that is classified as an important commodity and has high economic value. One of the main problems in the production of red chili is anthracnose disease caused by the Colletotrichum capsici pathogen. Bacillus spp. expected to have the potential to inhibit the growth of C. capsici and stimulate the growth of plant. This study aims to determine the potential of Bacillus spp., in suppressing the growth of the C. capsici fungus and stimulating the growth of red chili. This study used a Completely Randomized Design (CRD) with the treatment without Bacillus spp., chemical fungicides, Bacillus sp. (Ba-6), Bacillus sp. (Ba-9), Bacillus sp. (Ba-12), Bacillus sp. (Ba-15), and Bacillus sp. (Ba-17). The treatment was repeated 4 times and the treatment unit contained 5 red chili/polybag. The results showed that the highest suppression of C. capsici in the treatment of Bacillus sp. Ba-15 was 6.25% compared to the negative control. The best bacteria that can stimulate the growth of red chili is Bacillus sp. Ba-9 with an average plant height 20.10 cm and an average number of leaves 10.49
Co-Authors Arika Purnawati Arika Purnawati Herry Nirwanto Herry Nirwanto Nensi Agustina Rani, Sasiska Sasiska Rani Sri Wiyatiningsih Tri MUJOKO