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All Journal HAYATI Journal of Biosciences BIOMA : Jurnal Biologi Makassar
Juhriah
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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FITOREMEDIASI TANAMAN HIAS BUNGA Impatiens balsamina L., DAN Zinnia elegans (Jacq.) Kuntze TERHADAP POLUTAN MERKURI PADA TANAH: - Mutmainnah Zakariah Innah; Juhriah; Muhammad Ruslan Umar
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 8 No. 2 (2023): Bioma : Juli - Desember 2023
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Merkuri merupakan salah satu jenis logam yang banyak ditemukan di alam dan tersebar dalam batu-batuan, hasil tambang, tanah, air, dan udara sebagai senyawa anorganik dan organik. Merkuri dapat menjadi senyawa yang berbahaya jika mengalami metilisasi menjadi metil merkuri (MeHg) yang bersifat toksik bagi tubuh manusia. Penelitian bertujuan untuk melakukan fitoremediasi logam merkuri (Hg) pada tanah dengan menggunakan tanaman Impatiens balsamina L., dan Zinnia Elegans Jacq. Analisis kandungan merkuri (Hg) tanah (sebelum dan setelah fitoremediasi) dan jaringan tanaman menggunakan Inductively coupled plasma-mass spectrometry (ICP-MS). Kandungan merkuri (Hg) sebelum proses penanaman tanaman (Fitoremediasi) yaitu, sebesar 0,2621 µg/g. Kandungan Hg tanah setelah proses penanaman tanaman (Fitoremediasi) yaitu, pada tanaman hias bunga Zinnia elegans (Jacq.) Kuntze diperoleh 0,188 µg/g pada tanah dan 0,0641 µg/g pada tanaman, sedangkan pada tanaman hias bunga Impatiens balsamina L. diperoleh 0,1223 µg/g pada tanah dan 0,1641µg/g pada tanaman. Adapun hasil pengukuran dari biomassa tanaman, penyisihan logam dan efisiensi penyerapan logam. Biomassa tanaman yaitu Impatiens balsamina L. 86,90 % dan Zinnia elegans 75 % sedangkan efisiensi penyerapan logam Impatiens balsamina L. 134,17 % dan Zinnia elegans 54,32 %. Penyisihan logam pada tanaman hias bunga jenis Zinnia elegans 54, 97 % dan Impatiens balsamina 53,33 %. Kata kunci : fitoremediasi, merkuri, tanah, Impatiens balsamina, dan Zinnia Elegans Jacq.
Detection of the PR5 Gene Associated with Downy Mildew Resistance and  Genetic Diversity Analysis of S2 Lines of Local Maize (Zea mays L.) From South Sulawesi Using SSR Markers Rahma, Sitti; Juhriah; Agus, Rosana
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.475-489

Abstract

A significant decline in corn production is often associated with downy mildew (Peronosclerospora maydis), while maize varieties with genetic resistance to this disease are still relatively limited. A molecular-based approach is needed, such as Phatogenesis Related (PR5) gene detection, to identify maize genotypes that are potentially resistant to downy mildew. This study aims to identify the presence of the PR5 gene and analyze genetic diversity in local maize from South Sulawesi, Srikandi kuning (national variety), and Carotenoid sync 3 from International Maize and Wheat Improvement Center (CIMMYT) to support the acceleration of downy mildew-resistant plant breeding programs. PR5 gene detection was carried out by extracting RNA according to the Total RNA Mini Kit Plant (Geneaid) procedure followed by PCR techniques with specific primers. Genetic diversity analysis was carried out using 15 polymorphic SSR primers. DNA amplification showed that 23 individuals were detected as containing the PR-5 gene from 30 samples tested based on the results of agarose gel electrophoresis. The PIC value obtained from the Simple Sequence Repeats (SSR) primers showed a high level of genetic diversity ranging from 0.64 to 0.93 with an average of 0.85. The genetic similarity matrix was calculated and analyzed using the UPGMA method using NTSYS version 2.2, producing a dendrogram with two main clusters. Cluster I has only one individual with a large genetic distance, while Cluster II is divided into two subclusters, IIA and IIB, reflecting the genetic closeness of most individuals. Individuals with PR5 genes and high genetic diversity were identified as potential candidates for use in a superior maize breeding program resistant to downy mildew disease.
Co-Authors Mutmainnah Zakariah Innah Rosana Agus Sitti Rahma, Sitti Umar, Muhammad Ruslan