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Design of a Block-Shiped Photobioreactor Based on Microalgae Chlorella vulgaris for Capturing CO2 Emissions in Indoor Environments Adi Mulyana Supriatna; Dini Fujiyanti; Eko Prabowo Hadisantoso; Satiyarti, Rina Budi; Ashab Abdul Hakim
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 5 No. 2 (2025): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/tc83v884

Abstract

Carbon dioxide (CO2) is one of the air polluting gases that can cause various health problems if accumulated indoors. Therefore, finding solutions to capture CO2 indoors is important. One of the efforts to reduce CO2 is by placing microalgae as CO2 capture agents in photobioreactors. This study aims to produce a photobioreactor based on microalgae Chlorella vulgaris, analyse cell density, and measure CO2 absorption by microalgae in the photobioreactor. In this study, cultivation was carried out with continuous lighting, microalgae cell density was calculated using a haemocytometer observed under a microscope, and determination of dissolved CO2 levels using the acid-base titration method in Walne medium. The results showed that a block-shaped photobioreactor with a size of 10×10×27.5 cm and a thickness of 2 mm can be a solution to capture indoor CO2 gas. The density of microalgae cells in the photobioreactor was the highest at 4.9 107 (cells/mL), indicating active photosynthesis. The results also show that the photobioreactor has the ability to capture CO2 gas in the room which is characterised by an increase in dissolved ΔCO2 levels reaching 139.39 mg/L.  
Bioethanol Production from Ulva lactuca Macroalgae: Optimation of Acid Hydrolysis and Fermentation Time Assyifa Junitasari; Satiyarti, Rina Budi; RR. Nadya Shafa Afrida; Asep Supriadin
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/84yy9y51

Abstract

Nowadays bioethanol has been developed as a new renewable energy source of fuel because it has the same characteristics as ethanol, can increase the octane number in gasoline and be able to reduce CO2 emissions significantly. Bioethanol based on a natural ingredient of macroalgae can be used as fuel because it contains carbohydrates of glucose that can be fermented into alcohol through the process of acid hydrolysis. The study aims to identify the optimal fermentation time  and analyze the levels of bioethanol produced using GC-MS instruments from Ulva lactuca macroalgae type. The production of bioethanol was carried out using the acid hydrolysis method with H2SO4. The reducing sugar content was analyzed using the Luff-Schoorl method, followed by fermentation using Saccharomyces cerevisiae. Acid hydrolysis was conducted using 2 N H2SO4 at 80ºC for 120 minutes. Fermentation time was varied into three treatments, namely 72, 96, and 120 hours. Each treatment was performed in triplicate. The observed parameters included reducing sugar content, ethanol concentration, and bioethanol yield. Data obtained from the experiment were analyzed descriptively and presented as mean values to evaluate the effect of fermentation time on bioethanol production efficiency. The results of this study produced 15,72% reducing sugar and 0,9% bioethanol content. Accordingly, the best times of fermentation to produce bioethanol from Ulva lactuca macroalgae is for 72 hours