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All Journal Jurnal Teknologi Laboratorium Jurnal Biogenerasi
Anita Oktari, Anita
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Chemistry Education Languange, Linguistic, Communication & Media Other

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Pemeriksaan Golongan Darah Sistem ABO Metode Slide dengan Reagen Serum Golongan Darah A, B, O Oktari, Anita; Silvia, Nida Daeninur
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.325 KB)

Abstract

The standard examination blood type was performed using a reagent that actually less economical. This research in order to determine whether serum blood type can be used as a reagent blood type. It has been already conducted research antibodies in the serum of blood type A, B, and AB. Each serum would reacted with antigent in the blood, indicated by the agglutination. The research method was experimental by performing blood type with drops of serum in the blood type A, B and AB then saw the grade agglutination. The data obtained were processed statistically using Kruskal Wallis. The result showed serum A, B, and O can be used as a substitute reagents alternative anti – A, anti – B, and anti – AB in determining blood type. Based on analysis, quality clotting produced by serum aglutination is not same with the reagent anti – A, anti – B, and anti – AB in determining blood type.
Optimasi Air Perasan Buah Merah (Pandanus sp.) Pada Pemeriksaan Telur Cacing Oktari, Anita; Mu’tamir, Ahmad
Jurnal Teknologi Laboratorium Vol 6 No 1 (2017): 2017 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (870.914 KB) | DOI: 10.29238/teknolabjournal.v6i1.85

Abstract

Worm infections or can be called by worming included into an infection caused by a parasite. Soil Transmitted Helminths Intestinal Nematodes are worms groups are in their life cycle to reach the infective stage requires soil with certain conditions. Eosin 2% is the dye that is used in the examination of Intestinal Nematode worm eggs. Red fruit (Pandanus sp.) which is a natural plant material and acidic contain carotenoids which produces orange-red pigment. Beta carotene is the predominant pigment of red-orange color that is found naturally in plants and fruits. The aim of this research is to determine the best concentration from variation of red fruit (Pandanus sp.) juice that optimally to color the eggs of the worm. Research conducted experiments with various concentration ratio of red fruit (Pandanus sp.) juice and distilled water (1, 1:1, 1:2, 1:3, 1:4, 1:5). From this research it found that the results indicate the ratio of concentrations of red fruit (Pandanus sp.) juice and distilled water (1:2) can be used as an alternative reagent eosin 2% for their examination of worm eggs. But in the visual field that uses red fruit (Pandanus sp.) juice and distilled water (1:2) still looks much dirt as a nuisance and does not give a contrasting background. It can conclude that red fruit (Pandanus sp.) juice can use to color the eggs of the worm.
EKSTRAK ANTOSIANIN DARI BUAH STROBERI (FRAGARIA X ANANASSA) SEBAGAI PENGGANTI EOSIN PADA JARINGAN HATI TIKUS DENGAN PEWARNAAN HEMATOXYLIN-EOSIN Laila, Nazmy Noor; Mahmud, Dani; Oktari, Anita; Rukhiat, Diat
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6022

Abstract

Tissue staining is necessary to show the structure and morphology of the tissue, namely Hematoxylin Eosin (H&E) staining. However, eosin is carcinogenic when used in the long term. The aim of this research is expected to develop the use of natural materials as an alternative for Hematoxylin-Eosin histological staining. One of the color pigments that can be obtained from natural materials is the anthocyanin pigment found in strawberries. The anthocyanin extract from strawberries, which is acidic like eosin, will bind to positively charged molecules in the basic cytoplasm. The design of this study uses a simple experimental design (Posttest Only Design). Based on the Mann Whitney U test, a significant result was obtained with a value of sig.<.001 <0.05, indicating a significant difference in the results between the preparations stained with hematoxylin and eosin and those stained with hematoxylin and anthocyanin extract. Thus, it can be concluded that statistically, staining with hematoxylin and anthocyanin extract cannot be used as a substitute for hematoxylin eosin. Nevertheless, the results of staining the preparations using hematoxylin and anthocyanin extract still produce color in the cytoplasm, and are sufficiently contrasting to distinguish between the color of the cytoplasm and the color of the nucleus.
Co-Authors Laila, Nazmy Noor Mahmud, Dani Mu’tamir, Ahmad Nida Daeninur Silvia, Nida Daeninur Rukhiat, Diat