Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Teknologi Laboratorium Jurnal Teknokes
M Atik Martiningsih, M Atik
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Electrical & Electronics Engineering Engineering Environmental Science Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Gambaran Kadar Kolesterol Total Pada Penderita Hipertensi Sebeum Dan Sesudah Terapi Bekam Basah Sistiyono, Sistiyono; Martiningsih, M Atik; Hastuti, Febri
Jurnal Teknologi Laboratorium Vol 5 No 1 (2016): 2016 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.169 KB)

Abstract

Hipertensi merupakan faktor resiko utama dari perkembangan penyakit jantung dan stroke. Penyakit hipertensi berkembang secara perlahan, tetapi secara potensial sangat berbahaya. Hipertensi biasanya dikuti dengan penyakit lain yang menyertainya seperti hiperlipidemia Salah satu profil lipid yang dapat diukur adanya hiperlipidemia adalah kadar kolesterol total. Upaya pengendalian hipertensi yang disebabkan kadar kolesterol total yang tinggi yaitu dengan cara pengobatan baik sercara farmakologi maupun non-farmakologi. Pengobatan non-farmakologi salah satunya adalah terapi bekam basah.Penelitian ini bertujuan untuk mengetahui selisih rata-rata kadar kolesterol total pada penderita hipertensi sebelum, sesudah diberikan terapi bekam basah pertama dan kedua dengan pola tiga titik. Jenis penelitian yang digunakan adalah pre-experimental dengan desain penelitian pre and post test without control yaitu kadar kolesterol total sebelum dilakukan terapi bekam basah adalah pre test, sedangkan kadar kolesterol total setelah dilakukan terapi bekam basah adalah post test. Hasil didapatkan rata – rata kadar kolesterol total sebelum dilakukan terapi bekam basah adalah 194,89 mg/dl, setelah dilakukan terapi bekam basah pertama sebesar 200,89 mg/dl dan setelah dilakukan terapi bekam basah kedua menjadi 200, 33 mg/dl. Kesimpulann dari penelitian ini adalah selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah pertama adalah 6,00 mg/dl atau mengalami kenaikan sebesar 3,08% sedangkan selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah kedua adalah 5,44 mg/dl atau mengalami kenaikan sebesar 2,79%.
Isolation, Quantification, and Plaque Morphology Analysis of Lytic Bacteriophages from River Water Targeting Clinical MDR Klebsiella pneumoniae Using the Double-Layer Agar Method Aminah, Aminah; Trisna, Citra; Sugianto, Fitri Alina; Amalia, Gadis; Martiningsih, M Atik; Faruq, Zulfikar Husni
Jurnal Teknokes Vol. 18 No. 4 (2025): Desember
Publisher : Jurusan Teknik Elektromedik, Politeknik Kesehatan Kemenkes Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/jteknokes.v18i4.113

Abstract

Antimicrobial resistance is a growing global health threat and is projected to cause up to 10 million deaths per year by 2050. Klebsiella pneumoniae is a priority pathogen due to its multidrug resistance (MDR) mechanisms, such as extended-spectrum β-lactamases and carbapenemases, which significantly limit therapeutic options and increase the need for antimicrobial alternatives. This study aimed to isolate and quantify active lytic bacteriophages capable of infecting clinical MDR K. pneumoniae from river water samples. Water samples were processed by centrifugation and membrane filtration to remove debris and bacterial cells, then incubated with MDR K. pneumoniae in Luria broth at 37°C to enhance phage adsorption and amplification. Phage detection and enumeration were performed using the double-layer agar method. Plaque morphology was observed to confirm lytic activity, while serial dilutions were used to determine phage titer. Several lytic bacteriophages were successfully isolated from river water samples. The plaques formed were clear, spherical, and well-defined, with some exhibiting halos indicative of possible depolymerase activity. Phage titers ranged from 1.28 × 10³ to 2.00 × 10⁶ PFU/mL, indicating efficient replication against MDR K. pneumoniae without repeated enrichment processes. River water is a potential source of lytic bacteriophages capable of infecting MDR K. pneumoniae. These findings emphasize the role of aquatic environments as natural reservoirs of phages with potential use in the development of future antimicrobial or biocontrol strategies and support the need for further studies on the host range, stability, and therapeutic applications of the isolated phages.
Co-Authors Amalia, Gadis Aminah Aminah Febri Hastuti, Febri Sistiyono Sistiyono, Sistiyono Sugianto, Fitri Alina Trisna, Citra Zulfikar Husni Faruq