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All Journal Jurnal Teknologi Laboratorium Meditory : The Journal of Medical Laboratory Jurnal Analis Kesehatan Jurnal Kesehatan Health Information : Jurnal Penelitian Jurnal Info Kesehatan
Sistiyono Sistiyono, Sistiyono
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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PERBEDAAN KADAR KALSIUM PADA SERUM LIPEMIK DENGAN DAN TANPA PENAMBAHAN FLOKULAN GAMMA-SIKLODEKSTRIN INKUBASI SUHU 23 °C Niranata, Rosenadia Fitri Andafi; Sistiyono, Sistiyono; Setiawan, Budi
Jurnal Kesehatan Poltekkes Ternate Vol 10 No 2 (2017): Jurnal Kesehatan Poltekkes Ternate, November 2017
Publisher : UPPM Politeknik Kesehatan Kemenkes Ternate, Maluku Utara, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.426 KB) | DOI: 10.32763/juke.v10i2.43

Abstract

Serum lipemik merupakan serum berwarna keruh yang secara prinsip disebabkan oleh partikel besar lipoprotein seperti cylomicrons, VLDL (Verry Low Density Lipoprotein) maupun trigliserida. Serum lipemik umumnya dapat dikenali ketika kadar trigliserida di atas 300 mg/dl. Kekeruhan tersebut mengganggu absorbansi atau penghamburan cahaya pada berbagai pemeriksaan spektrofotometri. Gangguan ini dapat diatasi dengan flokulasi menggunakan gamma-siklodekstrin. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan kadar kalsium pada serum lipemik dengan dan tanpa penambahan flokulan gamma-siklodekstrin inkubasi suhu 23 °C. Penelitian ini merupakan penelitian kuantitatif dengan metode pra-eksperimen dan desain penelitian One-Group Pretest-Posttest Design. Bahan pemeriksaan yang digunakan dalam penelitian ini adalah serum lipemik sejumlah 20, dengan kadar >300 mg/dl. Hasil penelitian dianalisis secara deskriptif dan statistik dengan uji parametrik menggunakan Paired Sample T-Test. Rerata selisih kadar kalsium dalam serum lipemik degan dan tanpa penambahan gamma-siklodekstrin inkubasi suhu 23 °C adalah 5,47 mg/dl (30%). Hasil uji Paired Sample T-Test menunjukkan signifikan sebesar 0,000 (<0,05). Dari hasil tersebut dapat disimpulkan bahwa ada perbedaan kadar kalsium dalam serum lipemik dengan dan tanpa penambahan gamma-siklodekstrin inkubasi suhu 23 °C.
Pengaruh Berbagai Dosis Jus Buah Sirsak (Annona muricata L.) Terhadap Penurunan Kadar Kolesterol Low Density Lipoprotein (LDL) Serum Tikus Putih (Rattus norvegicus) Dislipidemia Tia, Hesti Dwi; Sistiyono, Sistiyono; Hendarta, Narendra Yoga
Jurnal Teknologi Laboratorium Vol 3 No 1 (2014): 2014 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.695 KB)

Abstract

Consumption of excessive fat in the food that we consume daily can increase cholesterol, LDL cholesterol and triglycerides in the bloodthat is called dyslipidemia. Diet, exercise and drugs are the effort can be done to lower the lipid levels. The herbs have been widely used as an alternative to lower cholesterol levels because the active substances contained antihiperlipid properties. Soursop has many benefits in curing some diseases. Soursop contains an active ingredient that can lower LDL’s cholesterol levels, including vitamin C, fiber and niacin. Therefore, the researchers wanted to do experiment using soursop as an alternative for lowering LDL’s cholesterol levels in dyslipidemia white rat serum. The purpose of this experiment was to determine the effect of various doses of soursop juice to decrease of LDL’s cholesterol levels in dyslipidemia white rat serum. This experiment used pre-test and post-test with control group design. Soursop juice doses used was at 0,9; 1,8 and 2,7 g / 200 g BW of white rat. The data were processed using descriptive and statistics by the Kolmogorov Smirnov Test, One Way ANOVA, Post Hoct Test and linear regression in SPSS 16.0 for Windows. The results of this experiment was the percentage decrease of LDL cholesterol levels in the white rat serum of various doses soursop juice, which is 0,9 g / 200 g BW of 46,19%, a dose of 1,8 g / 200 g BW of 52,30 % and a dose of 2,7 g / 200 g BW of 61,62%. There was the effect of various doses of soursop juice to decrease LDL’s cholesterol levels in dyslipidemia white rat serum. The greater the dose ofsoursop juice given, the greater the reduction in LDL’s cholesterol levels in the serum of dyslipidemia white rat.
Pengaruh Berbagai Dosis Jus Buah Sirsak (Annona muricata L.) Terhadap Penurunan Kadar Kolesterol Low Density Lipoprotein (LDL) Serum Tikus Putih (Rattus norvegicus) Dislipidemia Tia, Hesti Dwi; Sistiyono, Sistiyono; Hendarta, Narendra Yoga
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.296 KB)

Abstract

Consumption of excessive fat in the food that we consume daily can increase cholesterol, LDL cholesterol and triglycerides in the blood that is called dyslipidemia. Diet, exercise and drugs are the effort can be done to lower the lipid levels. The herbs have been widely used as an alternative to lower cholesterol levels because the active substances contained antihiperlipid properties. Soursop has many benefits in curing some diseases. Soursop contains an active ingredient that can lower LDL’s cholesterol levels, including vitamin C, fiber and niacin. Therefore, the researchers wanted to do experiment using soursop as an alternative for lowering LDL’s cholesterol levels in dyslipidemia white rat serum.The purpose of this experiment was to determine the effect of various doses of soursop juice to decrease of LDL’s cholesterol levels in dyslipidemia white rat serum.This experiment used pre-test and post-test with control group design. Soursop juice doses used was at 0,9; 1,8 and 2,7 g / 200 g BW of white rat. The data were processed using descriptive and statistics by the Kolmogorov Smirnov Test, One Way ANOVA, Post Hoct Test and linear regression in SPSS 16.0 for Windows.The results of this experiment was the percentage decrease of LDL cholesterol levels in the white rat serum of various doses soursop juice, which is 0,9 g / 200 g BW of 46,19%, a dose of 1,8 g / 200 g BW of 52,30 % and a dose of 2,7 g / 200 g BW of 61,62%.There was the effect of various doses of soursop juice to decrease LDL’s cholesterol levels in dyslipidemia white rat serum. The greater the dose of soursop juice given, the greater the reduction in LDL’s cholesterol levels in the serum of dyslipidemia white rat.
Gambaran Kadar Kolesterol Total Pada Penderita Hipertensi Sebeum Dan Sesudah Terapi Bekam Basah Sistiyono, Sistiyono; Martiningsih, M Atik; Hastuti, Febri
Jurnal Teknologi Laboratorium Vol 5 No 1 (2016): 2016 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.169 KB)

Abstract

Hipertensi merupakan faktor resiko utama dari perkembangan penyakit jantung dan stroke. Penyakit hipertensi berkembang secara perlahan, tetapi secara potensial sangat berbahaya. Hipertensi biasanya dikuti dengan penyakit lain yang menyertainya seperti hiperlipidemia Salah satu profil lipid yang dapat diukur adanya hiperlipidemia adalah kadar kolesterol total. Upaya pengendalian hipertensi yang disebabkan kadar kolesterol total yang tinggi yaitu dengan cara pengobatan baik sercara farmakologi maupun non-farmakologi. Pengobatan non-farmakologi salah satunya adalah terapi bekam basah.Penelitian ini bertujuan untuk mengetahui selisih rata-rata kadar kolesterol total pada penderita hipertensi sebelum, sesudah diberikan terapi bekam basah pertama dan kedua dengan pola tiga titik. Jenis penelitian yang digunakan adalah pre-experimental dengan desain penelitian pre and post test without control yaitu kadar kolesterol total sebelum dilakukan terapi bekam basah adalah pre test, sedangkan kadar kolesterol total setelah dilakukan terapi bekam basah adalah post test. Hasil didapatkan rata – rata kadar kolesterol total sebelum dilakukan terapi bekam basah adalah 194,89 mg/dl, setelah dilakukan terapi bekam basah pertama sebesar 200,89 mg/dl dan setelah dilakukan terapi bekam basah kedua menjadi 200, 33 mg/dl. Kesimpulann dari penelitian ini adalah selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah pertama adalah 6,00 mg/dl atau mengalami kenaikan sebesar 3,08% sedangkan selisih rata – rata kadar kolesterol total sebelum terapi bekam basah dengan setelah terapi bekam basah kedua adalah 5,44 mg/dl atau mengalami kenaikan sebesar 2,79%.
Penggunaan Kalium Feri Sianida Untuk Pengolahan Serum Ikterik Sujono, Sujono; Sistiyono, Sistiyono; Widada, Subrata Tri
Jurnal Analis Kesehatan Vol. 12 No. 1 (2023): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i1.3813

Abstract

A bad sample gives an invalid laboratory test result. There are several reasons that can cause a sample to be unfit for examination. The serum that most often causes errors are hemolysis, jaundice and lipemia. Icteric serum is serum that is yellow brown due to hyperbilirubinemia (increased levels of bilirubin in the blood). Icteric serum may cause interference with laboratory tests. Icteric serum can affect measurements at a wavelength of 400-500 nm. Examination by the method of oxidase or peroxidase reactions, such as glucose, cholesterol, triglycerides and uric acid can be disrupted. Potassium Ferricyanide is added to oxidize bilirubin to biliverdin before testing, and can reduce absorption disorders. Potassium ferric cyanide can reduce total bilirubin levels (p=0.000) in icteric serum. There were differences in the results of total cholesterol (p=0.000), triglycerides (p=0.019), glucose (p=0.001), total protein (p=0.011), albumin (p=0.004), globulin (p=0.002), creatinine ( p=0.011), urea (p=0.014), SGOT (p=0.007), SGPT (p=0.030) and alkaline phosphatase (p=0.002) in icteric serum added and not added potassium ferric cyanide.
Nilai Rujukan Darah Rutin Dewasa Sehat di Kota Yogyakarta Faruq, Zulfikar Husni; Nuryani, Siti; Sistiyono, Sistiyono; Rahmawati, Ullya
Health Information : Jurnal Penelitian Vol 17 No 1 (2025): Januari-April
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36990/hijp.v17i1.1471

Abstract

Darah rutin merupakan pemeriksaan yang hampir selalu dilakukan di rumah sakit. Perbedaan antara populasi rujukan yang terdapat pada alat dan reagen membuat nilai rujukan tidak selalu bisa digunakan di laboratorim. Penelitian ini bertujuan untuk melihat gambaran Nilai Rujukan Individu Dewasa Sehat di Kota Yogyakarta. Populasi penelitian menggunakan pendonor darah sehat dengan klasifikasi sesuai dengan uji saring donor darah. Pengambilan sampel dilakukan pada PMI Kota Yogyakarta. Sampel diuji menggunakan Hematology Analyzer dan data diuji menggunakan uji normalitas dan dilakukan pengujian uji beda. Pembuatan nilai rujukan menggunakan presentil 2,5-97,5%. Sampel penelitian pada penelitian ini berjumlah 241 orang terdiri dari 120 laki laki dan 121 wanita dengan rentang umur 17-70 tahun. nilai pria dan Wanita berturut turut adalah Hb (12.1-16.1; 11,5-15,3)g/dl, WBC (4,6-12,6; 5,3-13,3)x103 sel/?l, RBC (4.15-6,6; 3,9-5,49) x106 sel/?l, HCT (38-49,2; 35-45,5)%, MCV (75,9 – 92,39) fl, MCH (24,3 – 31,5)pg, MCHC (31,4-35,3)g/dl, MPV (8,3-11,2)fl dan PLT (184-453; 232-560). Nilai Rujukan pada darah rutin terdapat perbedaan dengan nilai rujukan yang umumnya digunakan di laboratorium sehingga diperlukan verifikasi nilai rujuk.
ALTERNATIVE MEDIA FOR BACTERIAL GROWTH STAPHYLOCOCCUS AUREUS: SWEET POTATO INFUSA (IPOMOEA BATATAS), SOYBEANS (GLYCINE MAX (L) MERRILL) AND YEAST EXTRACT Martsiningsih, Marta Atik; Astuti, Fardhiasih Dwi; Sistiyono, Sistiyono; Suyana, Suyana; Rahmawati, Ullya; Rahayu, Muji; Musyafaah, Novita Siti
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3081

Abstract

Background: Staphylococcus aureus needs nourishment for its growth. The needed nutrients of carbohydrates, proteins, nitrogen, vitamins, and minerals from the media. The media commonly used for bacterial growth is the nutrient medium for high prices. Alternative media from natural local foodstuffs are obtained easily and cheaply instead of instant media. This study aims to identify alternative media mixed with infuse sweet potato (ipomoea batatas), soybeans (glycine Max (l) Merrill), and yeast extract that can be used to grow the staphylococcus aureus bacteria.  Methode: this type of research is pure experimental research, and the design used is the posttest-only control design group. Staphylococcus aureus suspension was grown on alternative media and nutrient agar media as a comparison. measurements of colony diameter and number of colonies were compared between alternative media and nutrient agar. Result The results Research has provided an average number of staphylococcus aureus bacteria that grow on alternative media 78.00 x 104 CFU /ml and nutrient media for 94.19 x 104 CFU /ml. Whereas the colony's diameter of alternative media 2.56 mm, and the media nutrient increased by 2.66 mm. Conclusion: Alternative media from a blend of yam, soy infuse, and yeast extract can be used for the growth of the staphylococcus aureus bacteriaKeywords: Staphylococcus aureus; Alternative Media; Potato Infusa; soy infuse; soy infuse
Effect of The Temperature on The Size of Inhibition Zone of the Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim Activity Against Staphylococcus aureus ATCC 25923 Sistiyono, Sistiyono; Martsiningsih, Marta Atik; Kasiyati, Menik; Rahayu, Muji; Pudyastuti, Rita Rena; Suyana, Suyana; Rahmawati, Ully; Rahmad; Faruq, Zulfikar Husni; Setiawan, Budi
JURNAL INFO KESEHATAN Vol 22 No 3 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss3.1353

Abstract

Antibiotic sensitivity testing is essential for determining bacterial susceptibility to antibiotics. In disc diffusion testing, several technical factors influence the diameter of the inhibition zone, including incubation temperature, which must be carefully controlled to ensure the validity of test results. This study aims to determine the mean, difference, and analyze the diameter of the inhibition zone of the antibiotics, namely Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim against Staphylococcus aureus on Mueller-Hinton agar media with incubation temperatures of 33°C, 34°C, 35°C, 36°C and 37°C for 18 hours. This research is observational, with a cross-sectional design. The primary data is 100 data on the diameter of the antibiotic inhibition zone, obtained by measuring the diameter of the inhibition zone with different incubation temperatures. The selection of antibiotics is based on the mechanism of action of antibiotics inhibiting bacteria, namely the cell wall or cell membrane that surrounds the bacterial cell; the pieces of machinery that make the nucleic acids DNA and RNA, and the machinery that produces proteins (the ribosome and associated proteins) with a range of inhibition zones based on Internal Quality Control CLSI. The data and the repeated measure statistical test will be processed univariately to determine the significance of the difference in the diameter of the formed inhibition zone using the ANOVA test. The measurement of the inhibition zone diameter on the incubation temperature variation showed a significant difference with a p-value of 0.000 for Levofloxacin, Tetracycline, and Trimethoprim, while the p-value of Clindamycin is 0.010. For the other antibiotics, Levofloxacin, Tetracycline, and Trimethoprim antibiotics, the higher the incubation temperature, the average diameter of the inhibition zone is condensed, while for Clindamycin the higher the incubation temperature, the higher the average diameter of the inhibition zone is the same. Incubation temperature and volume affect the diameter of the antibiotic inhibition zone in the disc diffusion method for the antibiotic sensitivity test. It can be concluded that incubation temperature affects the diameter of the antibiotic inhibition zone in disc diffusion tests. It is recommended for future standardized and precise testing conditions to ensure accurate and reliable antibiotic sensitivity results.              
Co-Authors Budi Setiawan Fardhiasih Dwi Astuti Febri Hastuti, Febri Hesti Dwi Tia, Hesti Dwi Hieronymus Rayi Prasetya, Hieronymus Rayi Kasiyati, Menik M Atik Martiningsih, M Atik Maria Elisabeth Enjel Naur, Maria Elisabeth Enjel Maria Irena Dentri, Maria Irena Martsiningsih, Marta Atik Muji Rahayu Musyafaah, Novita Siti Narendra Yoga Hendarta, Narendra Yoga Niranata, Rosenadia Fitri Andafi Pudyastuti, Rita Rena Rahmad Rahmawati, Ully Siti Nuryani, Siti Subrata Tri Widada, Subrata Tri Sujono Sujono, Sujono Suyana Suyana, Suyana Ullya Rahmawati Zulfikar Husni Faruq