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All Journal Junior Medical Journal Indonesian Journal of Endocrinology Metabolism and Diabetes (InaJEMD)
Fatimah Eliana
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Religion Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Overview of Antibiotic Sensitivity Patterns in Diabetic Ulcers at Gatot Soebroto Army Hospital and Fatmawati General Hospital and The Review According to Islamic Perspective Sherly Idhalhijah; Fatimah Eliana; Andri Gunawan
Junior Medical Journal Vol 1, No 6 (2023)
Publisher : Junior Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v1i6.3133

Abstract

Backgorund : One of the complications of DM is diabetic ulcers which occur due to reduced pain sensation due to neuropathy. Diabetic ulcers are a state of finding infection, destruction to the deepest skin tissue in the feet in patients with diabetes mellitus (DM) due to nerve abnormalities and peripheral arterial vascular disorders, so an overview of the pattern of antibiotics is very important in overcoming the severity of ulcers that can expand.Methodolgy : This research used secondary data from medical records on the antibiotic sensitivity of diabetic foot ulcer patients at the Gatot Subroto Army Hospital and Fatmawati General Hospital from January 2018 to December 2021.Results : There are ten types of antibiotics that sensitive to diabetic foot ulcer patients at Gatot Soebroto Army Hospital, including tigecycline, cefepime, piperacillin tazobactam, amikacin, aztreonam, ceftazidime, ciprofloxacin, gentamicin, meropenem, and ceftriaxone. While the types of antibiotics at Fatmawati General Hospital, include amikacin, piperacillin tazobactam, imipenem, meropenem, ertapenem, gentamicin, trimethophrim sulfamethoxazole, tigecycline, fosfomycin, and cefepime.Conclusion : Sensitive antibiotics in the two hospitals include tigecycline, cefepime, piperacillin tazobactam, amikacin, gentamicin and meropenem. In the view of Islam treatment is a form of effort to seek healing, after which surrender everything to Allah SWT.Keywords : Antibiotic, diabetic ulcer, Army hospital, Fatmawati
Perubahan Gaya Hidup pada Pasien Diabetes Melitus Sebelum dan Saat Pandemi COVID-19 dan Tinjauannya Menurut Pandangan Islam Alyka Sheila Masah; Fatimah Eliana; Amir Mahmud
Junior Medical Journal Vol. 2 No. 10 (2024): Juni 2024
Publisher : Fakultas Kedokteran Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v2i10.4249

Abstract

Pandemi COVID-19 merupakan suatu outbreak yang menimbulkan gejala gangguan pernapasan akut yang disebabkan oleh novel coronavirus yang pertama kali ditemukan di Wuhan, Provinsi Hubei, Tiongkok. Pada masa pandemi, masyarakat terpaksa untuk lebih sering berdiam di rumah dan membatasi pergerakan luar rumah. Hal ini dapat merubah gaya hidup masyarakat. Penyandang diabetes melitus mempunyai risiko lebih tinggi terhadap COVID-19 karena dapat memperburuk kondisi bahkan menyebabkan kematian. Pandemi COVID-19 mengakibatkan sebagian besar orang terpaksa untuk merubah gaya hidup mereka terutama pada pasien diabetes melitus. Jenis penelitian yang dilakukan adalah penelitian deskriptif analitik dengan rancangan cross-sectional. Cara pengambilan sampel menggunakan kuesioner dengan teknik wawancara. Sampel pada penelitian ini adalah pasien diabetes melitus di Poli Penyakit Dalam Rumah Sakit Umum Pusat Fatmawati. Analisis secara statistik menggunakan uji marginal homogeneity ditemukan tidak ada perubahan pada variabel pola makan (p=0,439) dan kebiasaan (p=0,248). Variabel aktivitas fisik (p=0,000), pola tidur (p=0,008), dan tingkat stres (p=0,000) menunjukkan adanya perubahan bermakna sebelum dan saat pandemi COVID-19. Secara keseluruhan tidak ditemukan perubahan gaya hidup karena p-value 0,3465 (p>0,05). Pada penelitian ditemukan perubahan aktivitas fisik, pola tidur, dan tingkat stress pada pasien diabetes melitus sebelum dan saat pandemi COVID-19. Dalam pandangan Islam, ketika sedang menderita suatu penyakit seperti diabetes melitus, maka seorang pasien diabetes melitus yang beragama Islam harus menghadapi keadaan ini dengan sifat-sifat yang telah di ajarkan dalam Islam seperti sabar, tawakal, ridha, dan ikhtiyar.
Isolation, In Vitro Expansion, And Cryopreservation of Primary Cells Derived from Human Thyroid Carcinoma Fatimah Eliana; Sabrina Azmi; Anggi Puspa Nur Hidayati; Juhan Khalila
‎ InaJEMD - Indonesian Journal of Endocrinology Metabolic and Diabetes Vol. 2 No. 1 (2025): InaJEMD Vol. 2, No. 1
Publisher : PP PERKENI

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Abstract

Thyroid carcinoma is a malignant neoplasm arising from thyroid parenchymal cells and currently ranks as the fourth most diagnosed cancer in Indonesia. This study aimed to isolate thyroid carcinoma cells for in vitro expansion and long-term preservation as a reliable cell culture stock, including cryopreservation for future research applications. In addition, we sought to identify and characterize cells derived from papillary thyroid carcinoma (PTC) tissue to evaluate the presence of mutations with potential prognostic significance. Primary cell isolation was performed via enzymatic digestion using collagenase, enabling effective separation of tumor cells from adjacent non-malignant thyroid tissue. Cell proliferation was supported using Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 20% fetal bovine serum (FBS), selected for its high concentration of growth-promoting factors that enhance proliferation rates. For biobanking purposes, cryopreservation of the thyroid carcinoma-derived cells was conducted using a standard slow-freezing protocol. Molecular characterization was carried out through PCR amplification, gel electrophoresis, and Sanger sequencing of key oncogenic drivers, specifically the BRAF gene and five RAS gene targets: HRAS exon 2, NRAS exons 2 and 3, and KRAS exons 2 and 3. No pathogenic mutations were identified in the analyzed BRAF or RAS gene regions.
Co-Authors Alyka Sheila Masah Amir Mahmud Andri Gunawan Anggi Puspa Nur Hidayati Juhan Khalila Sabrina Azmi Sherly Idhalhijah