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Species Identification among Fish Samples taken from Mangrove Ecosystem in Lampung Coastal Bay through DNA Barcoding Technique Yanti Ariyanti; Ika Agus Rini; Novriadi Novriadi; Fidya Septiana
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 2 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.2.173-180

Abstract

Mangrove forests are essential for supporting the habitats of numerous fish species, some of which are vital for local economies. These forests serve as breeding and nurturing environments for fish, providing a safe haven for juvenile fish and protecting them from predators and environmental stressors. There are many different species of fish that use mangrove forests as a breeding and nursery ground. Although many studies on the biodiversity of mangrove organisms on Sumatra Island exist, data on fish species diversity in Lampung Bay is still lacking. Morphological identification is often inadequate to distinguish between potential fish species, even across different life stages such as adults, juveniles, or larvae. However, DNA barcoding has the potential to identify species at any developmental stages accurately. Thus, this study aimed to identify and confirm fish species originating from the mangrove ecosystem in Lampung Bay specifically the Sebalang Mangrove Ecopark (South Lampung) and Petengoran Mangrove Forest, Gebang Village (Pesawaran), through a molecular approach using DNA barcoding techniques. Twenty samples can be identified at the species/genus level, demonstrating that using short, standardized genetic region Cytochrome c oxidase I (COI) mitochondrial gene sequences can accurately and quickly classify fish samples. Furthermore, twenty mitochondrial DNA sequences of various fishes have been submitted to the massive genetic database, GenBank. By identifying species accurately and quickly, DNA barcoding can improve the knowledge about fish biodiversity especially samples taken from the mangrove ecosystem in Lampung Coastal Bay.
SURVEI TUTUPAN KARANG DAN KOMPOSISI IKAN KARANG DI PERAIRAN PLTU SEBALANG, LAMPUNG SELATAN M Khairul Anam; Novriadi Novriadi
MAXIMUS: Journal of Biological and Life Sciences Vol 2 No 2 (2024)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/maximus.v2i2.1971

Abstract

Terumbu karang merupakan ekosistem produktif yang menghadapi ancaman kerusakan akibat penangkapan ikan ilegal dan pembangunan pesisir yang tidak berkelanjutan. Praktik penangkapan ikan ilegal dan pembangunan area pesisir diduga berkontribusi pada penurunan tutupan karang hidup di perairan PLTU Sebalang, Lampung Selatan. Informasi mengenai kondisi ekosistem terumbu karang di area PLTU Sebalang Lampung Selatan saat ini masih belum memadai. Oleh sebab itu perlu dilakukan survei kesehatan terumbu karang di area ini sebagai bagian dari langkah pengelolaan ekosistem terumbu karang secara berkelanjutan. Penelitian ini dilakukan pada bulan Januari 2024 di perairan PLTU Sebalang. Pengambilan data terumbu karang menggunakan metode Underwater Photo Transect (UPT) yang dikombinasikan dengan program identifikai karang CPCe (Coral Point Count with Excel extensions). Pengambilan data ikan karang menggunakan metode Underwater Visual Census (UVC). Hasil penelitian ini menunjukkan kondisi tutupan terumbu karang pada seluruh titik penelitian dikategorikan buruk. Persen tutupan karang terendah berada pada titik 1 kedalaman 8 meter yaitu sebesar 1.53%. Sedangkan kondisi tutupan karang pada titik 2 kedalaman 8 meter memiliki persentase tertinggi yaitu sebesar 23.28%. Ikan karang yang ditemukan sebanyak 10 famili yang terdiri dari Apogonidae, Aulostomidae, Caesonidae, Chaetodontidae, Haemulidae, Holocentridae, Labridae, Nemipteridae, Pomacentridae, dan Pempherididae. Faktor yang mempengaruhi buruknya kondisi tutupan terumbu karang dan sedikitnya jumlah individu ikan karang yang terdapat pada seluruh titik pengamatan dipengaruhi oleh faktor tingginya sedimentasi, kuatnya arus perairan, dan nelayan jaring payang yang menangkap ikan hingga ke dasar perairan. Coral reefs are productive ecosystems that face significant threats from illegal fishing and unsustainable coastal development. These practices are suspected of contributing to the decline in live coral cover in the waters surrounding the Sebalang Power Plant (PLTU Sebalang), South Lampung. Current information on the coral reef ecosystem in this area remains insufficient. Therefore, a coral health survey is essential as part of sustainable management efforts. This study was conducted in January 2024 in the waters of PLTU Sebalang. Coral reef data were collected using the Underwater Photo Transect (UPT) method, combined with the Coral Point Count with Excel extensions (CPCe) software for coral identification. Data on reef fish were gathered using the Underwater Visual Census (UVC) method. The results indicate that coral reef cover at all study sites was categorized as poor. The lowest coral cover was recorded at Site 1 at a depth of 8 meters, with only 1.53% coverage, while the highest cover was observed at Site 2, also at a depth of 8 meters, with 23.28%. A total of 10 reef fish families were identified, including Apogonidae, Aulostomidae, Caesonidae, Chaetodontidae, Haemulidae, Holocentridae, Labridae, Nemipteridae, Pomacentridae, dan Pempherididae. The poor condition of coral cover and the low number of individual reef fish at all observation sites were influenced by high sedimentation, strong currents, and the use of payang nets by fishermen, which reach the seabed.