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Species Identification among Fish Samples taken from Mangrove Ecosystem in Lampung Coastal Bay through DNA Barcoding Technique Yanti Ariyanti; Ika Agus Rini; Novriadi Novriadi; Fidya Septiana
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 2 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.2.173-180

Abstract

Mangrove forests are essential for supporting the habitats of numerous fish species, some of which are vital for local economies. These forests serve as breeding and nurturing environments for fish, providing a safe haven for juvenile fish and protecting them from predators and environmental stressors. There are many different species of fish that use mangrove forests as a breeding and nursery ground. Although many studies on the biodiversity of mangrove organisms on Sumatra Island exist, data on fish species diversity in Lampung Bay is still lacking. Morphological identification is often inadequate to distinguish between potential fish species, even across different life stages such as adults, juveniles, or larvae. However, DNA barcoding has the potential to identify species at any developmental stages accurately. Thus, this study aimed to identify and confirm fish species originating from the mangrove ecosystem in Lampung Bay specifically the Sebalang Mangrove Ecopark (South Lampung) and Petengoran Mangrove Forest, Gebang Village (Pesawaran), through a molecular approach using DNA barcoding techniques. Twenty samples can be identified at the species/genus level, demonstrating that using short, standardized genetic region Cytochrome c oxidase I (COI) mitochondrial gene sequences can accurately and quickly classify fish samples. Furthermore, twenty mitochondrial DNA sequences of various fishes have been submitted to the massive genetic database, GenBank. By identifying species accurately and quickly, DNA barcoding can improve the knowledge about fish biodiversity especially samples taken from the mangrove ecosystem in Lampung Coastal Bay.
Species of Sea Urchins (Echinoidea) in the Intertidal Zone of Pahawang Island, Pesawaran, Lampung Jeane Siswitasari Mulyana; Gres Maretta; Muhammad Aqil Maulana; Novriadi Novriadi; Andy Darmawan
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.6544

Abstract

Background: Sea urchins are susceptible to environmental changes, including ones caused by anthropogenic activities in coastal area. They are commonly found in coral reef areas, seagrass beds, and mixed sand and coral rubble substrates. These marine ecosystems face critical problem as a result of water pollution, thus affecting their ecological roles as habitats for sea urchins. Pahawang Island is located in the Lampung Bay area and has substrate characteristics that are suitable for the habitat preferences of sea urchins. On the contrary, Pahawang Island becomes famous tourist destination and this can potentially cause damage to habitats of sea urchin. The purpose of this research is to identify sea urchin species (Echinoidea) in the intertidal zone of Pahawang Island, Lampung. Methodology: Samples were collected using surveying method by walking along the intertidal zone during low tide. Sampling locations include Jelarangan Hamlet, Suwak Buah Hamlet, Andreas Resort around Kalangan Hamlet, and Pahawang Hamlet. Sea urchins were identified based on morphological characteristics. Findings: Sea urchins found in the intertidal zone of Pahawang Island consist of four species: Echinothrix calamaris, Diadema setosum, and Diadema antillarum, and Mespilia globulus. E. calamaris and D. setosum were found on substrates such as seagrass beds, coral, and sand, while Diadema antillarum and Mespilia globulus were only found in waters with coral substrates. These four species of sea urchins inhabit diverse habitats and play important ecological roles in the ecosystem, even influencing the conditions of other benthic organism communities in intertidal zone of Pahawang Island. Contribution: This study provides baseline data on sea urchin species within specific area to later plan conservation strategies related to human-induced environmental changes.