Liviani Rangian
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Amplification Of Bacterial Isolate Sf1 Associated With Sponge Facaplysynopsis sp. From Tongkeina, North Sulawesi Liviani Rangian; Elvy Like Ginting; Stenly Wullur; Erly Kaligis; Sandra Tilaar; Reiny Tumbol
Jurnal Ilmiah PLATAX Vol. 6 No. 2 (2018): ISSUE JULY-DECEMBER 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.6.2.2018.20636

Abstract

This study was conducted with the aim of amplifying the isolate of SF1 symbiont sponge Facaplysynopsis sp.  from Tongkeina, North Sulawesi. Samples are obtained and stored in the Lab. Molecular Biology and Marine Pharmacology, FPIK Unsrat. The genomic DNA of the samples was isolated using protocols from the Innu PREP Mini DNA Kit. The DNA of the SF1 symbionary bacteria was amplified by PCR (Polymerase Chain Reaction) using an 8F primer (5'-AGAGTITGATCCTGGCTA-3 ') and 1492 R (5'TACCTTACGACTT-3'). DNA bacteria SF1 successfully amplified marked by the appearance of the band of DNA that looks less clear, with a length of 600 bp.Keywords: Bacterial Isolate, Sponge,  AmplificationABSTRAKPenelitian ini dilakukan dengan tujuan mengamplifikasi isolat bakteri SF1 simbion spons Facaplysynopsis sp dari perairan Tongkeina, Sulawesi Utara. Sampel diperoleh dan tersimpan di Lab. Biologi Molekuler dan Farmasitika Laut, FPIK Unsrat. DNA genom dari sampel diisolasi menggunakan protokol dari Innu PREP DNA Mini Kit. DNA bakteri simbion SF1 diamplifikasi dengan PCR (Polymerase Chain Reaction) dengan menggunakan primer 8F (5’-AGAGTITGATCCTGGCTA-3’) dan 1492 R (5’TACCTTACGACTT-3’). DNA bakteri SF1 berhasil diamplifikasi ditandai dengan munculnya pita DNA yang terlihat kurang jelas, dengan panjang 600 bp.Kata Kunci: Bakteri Simbion, Spons, Amplifikasi
Isolation of Symbiotic Bacteria with Red Algae from Tongkaina Waters, North Sulawesi Elvy Like Ginting; Liviani Rangian; Letha L. Wantania; Stenly Wullur
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23728

Abstract

This study aims to isolate symbiotic bacteria with red algae from the waters of Tongkaina, North Sulawesi. The red algae taken were red algae that are similar to the genus Portieria and Gracilaria. Bacteria were cultured and isolated using Nutrient Agar (NA) + sea water. Before bacteria from red algae samples were cultured, each sample of red algae was crushed, homogenized and diluted. The results of this study, 5 bacterial isolates were successfully isolated from red algae similar to Portieria sp. and 5 bacterial isolates from red algae similar to Gracilaria sp. Each bacterial isolate has different morphological characteristics such as shape, colour, elevation, and edges. Of the 10 bacterial isolates, 3 bacterial cells are gram-positive cocci (round), and 7 of them are gram-negative bacilli (rod).Keywords: Red algae, bacteria, isolation, symbionts ABSTRAKTujuan dari penelitian ini adalah untuk mengisolasi bakteri simbion dengan alga merah dari perairan Tongkaina, Sulawesi Utara. Alga merah yang diambil adalah alga merah yang mirip dengan genus Portieria dan Gracilaria. Bakteri ditumbuhkan dan diisolasi menggunakan media Nutrien Agar (NA) + air laut.  Sebelum bakteri dari sampel alga merah ditumbuhkan, masing-masing sampel alga merah digerus, dihomogeniasi dan diencerkan.  Hasil penelitian ini, 5 isolat bakteri berhasil diisolasi dari alga merah mirip Portieria sp. dan 5 isolat bakteri dari alga merah mirip Gracilaria sp. Masing-masing isolat bakteri memiliki karakteristik morfologi yang berbeda seperti bentuk, warna, elevasi, dan tepian. Dari 10 isolat  bakteri  tersebut, 3 sel bakteri bersifat gram positif dengan bentuk bulat, dan 7 diantaranya bersifat gram negatif dengan bentuk batang.Kata kunci:  Alga merah, bakteri, isolasi, simbion