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All Journal JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX
Elvy Like Ginting
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Earth & Planetary Sciences Environmental Science

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Amplification Of Bacterial Isolate Sf1 Associated With Sponge Facaplysynopsis sp. From Tongkeina, North Sulawesi Liviani Rangian; Elvy Like Ginting; Stenly Wullur; Erly Kaligis; Sandra Tilaar; Reiny Tumbol
Jurnal Ilmiah PLATAX Vol. 6 No. 2 (2018): ISSUE JULY-DECEMBER 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.6.2.2018.20636

Abstract

This study was conducted with the aim of amplifying the isolate of SF1 symbiont sponge Facaplysynopsis sp.  from Tongkeina, North Sulawesi. Samples are obtained and stored in the Lab. Molecular Biology and Marine Pharmacology, FPIK Unsrat. The genomic DNA of the samples was isolated using protocols from the Innu PREP Mini DNA Kit. The DNA of the SF1 symbionary bacteria was amplified by PCR (Polymerase Chain Reaction) using an 8F primer (5'-AGAGTITGATCCTGGCTA-3 ') and 1492 R (5'TACCTTACGACTT-3'). DNA bacteria SF1 successfully amplified marked by the appearance of the band of DNA that looks less clear, with a length of 600 bp.Keywords: Bacterial Isolate, Sponge,  AmplificationABSTRAKPenelitian ini dilakukan dengan tujuan mengamplifikasi isolat bakteri SF1 simbion spons Facaplysynopsis sp dari perairan Tongkeina, Sulawesi Utara. Sampel diperoleh dan tersimpan di Lab. Biologi Molekuler dan Farmasitika Laut, FPIK Unsrat. DNA genom dari sampel diisolasi menggunakan protokol dari Innu PREP DNA Mini Kit. DNA bakteri simbion SF1 diamplifikasi dengan PCR (Polymerase Chain Reaction) dengan menggunakan primer 8F (5’-AGAGTITGATCCTGGCTA-3’) dan 1492 R (5’TACCTTACGACTT-3’). DNA bakteri SF1 berhasil diamplifikasi ditandai dengan munculnya pita DNA yang terlihat kurang jelas, dengan panjang 600 bp.Kata Kunci: Bakteri Simbion, Spons, Amplifikasi
Isolation of Symbiotic Bacteria with Red Algae from Tongkaina Waters, North Sulawesi Elvy Like Ginting; Liviani Rangian; Letha L. Wantania; Stenly Wullur
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23728

Abstract

This study aims to isolate symbiotic bacteria with red algae from the waters of Tongkaina, North Sulawesi. The red algae taken were red algae that are similar to the genus Portieria and Gracilaria. Bacteria were cultured and isolated using Nutrient Agar (NA) + sea water. Before bacteria from red algae samples were cultured, each sample of red algae was crushed, homogenized and diluted. The results of this study, 5 bacterial isolates were successfully isolated from red algae similar to Portieria sp. and 5 bacterial isolates from red algae similar to Gracilaria sp. Each bacterial isolate has different morphological characteristics such as shape, colour, elevation, and edges. Of the 10 bacterial isolates, 3 bacterial cells are gram-positive cocci (round), and 7 of them are gram-negative bacilli (rod).Keywords: Red algae, bacteria, isolation, symbionts ABSTRAKTujuan dari penelitian ini adalah untuk mengisolasi bakteri simbion dengan alga merah dari perairan Tongkaina, Sulawesi Utara. Alga merah yang diambil adalah alga merah yang mirip dengan genus Portieria dan Gracilaria. Bakteri ditumbuhkan dan diisolasi menggunakan media Nutrien Agar (NA) + air laut.  Sebelum bakteri dari sampel alga merah ditumbuhkan, masing-masing sampel alga merah digerus, dihomogeniasi dan diencerkan.  Hasil penelitian ini, 5 isolat bakteri berhasil diisolasi dari alga merah mirip Portieria sp. dan 5 isolat bakteri dari alga merah mirip Gracilaria sp. Masing-masing isolat bakteri memiliki karakteristik morfologi yang berbeda seperti bentuk, warna, elevasi, dan tepian. Dari 10 isolat  bakteri  tersebut, 3 sel bakteri bersifat gram positif dengan bentuk bulat, dan 7 diantaranya bersifat gram negatif dengan bentuk batang.Kata kunci:  Alga merah, bakteri, isolasi, simbion
ISOLASI BAKTERI LAUT DARI PERAIRAN MALALAYANG, SULAWESI UTARA Bella Wondal; Elvy Like Ginting; Veibe Warouw; Stenly Wullur; Sandra Olivia Tilaar; Ferdinand Frans Tilaar
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24448

Abstract

Marine bacteria have a lot of potential in exploring the enzyme that can be developed, such as a producer of proteorhodopsin, act as hydrocarbon chlorlastic and can degrade oil. This study aims to obtain isolates and can characterize the bacterial morphology. Malalayang Waters is one of the marine bacterial habitats that has potential area to be studied. This study aims to isolate marine bacteria from Malalayang Waters. These marine bacteria first were diluted into sea water before they were grown on Nutrient Agar (NA). Based on the results of this study it was found that marine bacterial isolated were separated based on their morphological characteristics. The dominant morphological characteristics were yellow whites which dominant shape were irregular.Keywords: bacterial, dilution, isolation.  Bakteri laut memiliki banyak potensi yang dapat dikembangkan. Seperti penghasil proteorhodopsin, berperan sebagai hidrokarbonoklastik dan dapat mendegradasi minyak. Perairan Malalayang merupakan salah satu habitat bakteri laut yang belum diteliti. Penelitian ini bertujuan untuk mengisolasi bakteri laut dari Perairan Malalayang. Bakteri laut ditumbuhkan pada media agar + air laut, selain itu bakteri juga dilakukan pengenceran terhadap air laut sebelum bakteri ditumbuhkan pada media Nutrient Agar (NA). Berdasarkan hasil penelitian ini isolat bakteri  laut ditemukan, bakteri tersebut dipisahkan berdasarkan karakteristik morfologinya. Karakteristik morfologi yang dimiliki dominan berwarna putih kuning dan memiliki bentuk yang dominan tidak teratur. Hal ini dapat memperlihatkan perbedaan bakteri laut dari Perairan Malalayang yang tumbuh.Kata kunci: bakteri, pengenceran, isolasi.
KARAKTERISTIK MORFOLOGI BAKTERI SIMBION SPONS MENYERUPAI Cribochalina sp DARI PERAIRAN MALALAYANG SULAWESI UTARA Gledys Giacinta Poluan; Elvy Like Ginting; Stenly Wullur; Veibe warouw; Fitje Vera Losung; Meiske Salaki
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24452

Abstract

Bacteria are found to be highly associated with various marine organisms, such as sponges. Sponges are known as the product of bioactive compounds. However, some of the compounds produced by sponges are obtained by the simbion-bacteria. Therefore this situation enables sponges simbion bacteria to play a major role in producing bioactive compounds that have been isolated from the sponge. The purpose of this study is to isolate and determine the characteristics of morphology of spongy simbion bacteria resembling Cribochalina sp, which was taken from Malalayang waters, North Sulawesi. Sponge simbion bacteria grow on NB media. Initially, a free-breeding colony was being done before bacteria were accrued in the NA media by 2% sponge broth (patent SID201906301) by means of Strike Plate methods. Based on to this study we had isolate five spongy simbion bacteria that resembling the Cribochalina sp. All five of these isolations have different characteristics of morphology in terms of color, shape, size, and elevation.      Keywords : bacteria, isolation, simbionts, sponges 
Co-Authors Bella Wondal Erly Kaligis Ferdinand Frans Tilaar Fitje Vera Losung Gledys Giacinta Poluan Letha L. Wantania Liviani Rangian Meiske Salaki Reiny Tumbol Sandra Olivia Tilaar Sandra Tilaar Stenly Wullur Veibe Warouw