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All Journal Agrica: Journal of Sustainable Dryland Agriculture Jurnal Agrotropika Agroradix : Jurnal Ilmu Pertanian
Sugiarto Sugiarto
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Singaperbangsa Karawang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry

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Pertumbuhan Metarhizium rileyi dengan Penambahan Biji Sirsak dan Umbi Gadung untuk Mendukung Laju Pertumbuhan Cendawan Entomopatogen Safira Nurmala Senja; Lutfi Afifah; Sugiarto Sugiarto; Anik Kurniati
JURNAL AGROTROPIKA Vol 22, No 1 (2023): Jurnal Agrotropika Vol 22 No 1, Mei 2023
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v22i1.7452

Abstract

Mikroorganisme yang dapat dimanfaatkan sebagai agens pengendalian hama secara hayati salah satunya adalah cendawan entomopatogen Metarhizium rileyi. Penambahan ekstrak biji sirsak dan umbi gadung sebagai media alternatif yang kaya akan nutrisi mampu memengaruhi pertumbuhan cendawan entomopatogen M. rileyi. Penelitian ini bertujuan untuk mendapatkan media pertumbuhan terbaik cendawan M. rileyi yang dicampur dengan ekstrak biji sirsak dan umbi gadung. Metode yang digunakan adalah metode eksperimental dan rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) faktor tunggal yang terdiri dari 7 perlakuan dan diulang 4 kali. Perlakuan tersebut antara lain Kontrol (PDA), PS1 (PDA + ekstrak biji sirsak 1 ml/l), PS3 (PDA + ekstrak biji sirsak 3 ml/l), PS5 (PDA + ekstrak biji sirsak 5 ml/l), PG1 (PDA + ekstrak umbi gadung 1 g/l), PG3 (PDA + ekstrak umbi gadung 3 g/l), PG5 (PDA + ekstrak umbi gadung 5 g/l). Hasil yang dicapai dari penelitian ini adalah penambahan ekstrak biji sirsak dan umbi gadung ke dalam media tumbuh M. rileyi memberikan pengaruh yang tidak nyata terhadap diameter koloni 21 hsi dengan rata-rata diameter koloni berkisar 8,95-9,00 cm, sedangkan hasil analisis regresi dan waktu inkubasi selama 21 hari berpengaruh nyata terhadap laju pertumbuhan koloni M. rileyi sebesar 44% hingga 77%. Hubungan laju pertumbuhan koloni M. rileyi dengan waktu inkubasi pada semua perlakuan menunjukkan nilai R2 sebesar 0,44 hingga 0,77. Media paling efektif untuk pertumbuhan cendawan M. rileyi adalah media PDA dengan ekstrak umbi gadung 1 g/l dengan diameter koloni 9,00 cm.
Pengaruh Akar Tuba (Derris elliptica) Terhadap Mortalitas Dan Intensitas Serangan Keong Mas (Pomacea canaliculata L.) Pada Padi Widya Ningrum; Lutfi Afifah; Sugiarto Sugiarto; Anton Yustiano
AGRICA Vol. 16 No. 2 (2023): December
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v16i2.3032

Abstract

Rice (Oryza sativa L.) is a rice-producing plant that is one of the staple foods for the world's population, especially Indonesia. One of the factors causing a decrease in rice production is pests, the main pest of rice plants is the golden snail. The golden snail pest attack occurs on rice plant seedlings, namely in the vegetative phase. The purpose of this study was to determine the effect of giving plant-based pesticides tuba root extract (Derris elliptica) on the mortality and pest intensity of the golden snail (Pomacea canaliculata L.) on rice plants (Oryza sativa L.) Inpari 32 variety. The method used was experimental (trial) using a single factor Randomized Block Design (RBD) with 6 treatments with 5 replications so there were 30 experimental units. Treatment of D. elliptica tuba root extract concentration on rice (Oryza sativa L.) treatment A (Control), B (5g/L), C (7g/L), D (9g/L), E ( 11g/L), and F (13g/L). Tuba root extract at a concentration of 13g/L is the best concentration which gives the highest mortality of 81.60% with the lowest attack intensity value of 29.84%. The LC50 value of the tuba root extract on the golden snail was 2.64g/L, the minimum value was 0.37g/L and the maximum was 4.164 g/L. LT50 takes a minimum of 1 day and a maximum of 9 days with an average time of 6-5 days. The results of calculating the correlation between mortality and the intensity of golden snail attacks on tall rice plants are -0.60. Administration of tuba root extract of D. eliptica was effective against the mortality of the golden snail P. canaliculata and the intensity of the attack by the golden snail P. canaliculata on rice (Oryza sativa L.).
IDENTIFIKASI JAMUR ANTAGONIS ASAL MEDIA LIMBAH JAMUR MERANG DAN POTENSINYA DALAM MENEKAN Rhizctonia solani PENYEBAB PENYAKIT PENYAKIT HAWAR PELEPAH PADI (Oryza sativa L.) Ahmad Daffa Miftahussurur; Satriyo Restu Adhi; Sugiarto Sugiarto
AGRORADIX : Jurnal Ilmu Pertanian Vol 7 No 2 (2024): Juli 2024
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v7i2.6561

Abstract

One of the main diseases of rice is sheath blight caused by the fungus Rhizoctonia solani. The aim of this research is to isolate fungi originating from straw mushroom media waste which have antagonistic properties to the fungus R. solani. The research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, Singaperbangsa University Karawang. The stages of this research consisted of: (1) isolation and identification of antagonist fungi from straw mushroom media waste, (2) in-vitro antagonism test using a Completely Randomized Design (CRD) repeated three times, (3) pathogenicity test of antagonist fungi on germination rice seeds. From the experimental results, 9 isolates of antagonistic fungi were obtained, 7 fungal genera were identified, consisting of 4 genera Trichoderma sp. and 3 genera Aspergillus sp. Based on the AUCGC results and the percentage of inhibition, the results show that several fungi from straw mushroom media waste have the ability to antagonize and inhibit R. solani. Treatment of JMA2 (Trichoderma sp.) and R. solani in the antagonism test gave the smallest AUCGC value of 29.38 and the largest inhibition percentage of up to 31.22%.
Co-Authors Ahmad Daffa Miftahussurur Anik Kurniati Anton Yustiano Lutfi Afifah Safira Nurmala Senja Satriyo Restu Adhi Widya Ningrum