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All Journal JFIOnline Pharmaceutical Sciences and Research (PSR) Makara Journal of Science
Atiek Sumiati, Atiek
Laboratorium Mikrobiologi dan Bioteknologi Departemen Farmasi FMIPA, Universitas Indonesia, Depok, 16424
Health Professions Medicine & Pharmacology

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MOLECULAR IDENTIFICATION OF ENDOPHYTIC FUNGI ISOLATED FROM Garcinia porrecta AND Garcinia forbesii Radji, Maksum; Nugraheni, Femi Arifah; Sumiati, Atiek
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 4, No 4 (2009)
Publisher : Indonesian Research Gateway

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Abstract

Penelitian ini dilakukan untuk mengidentifikasi isolat-isolat kapang endofit yang telah diisolasi dari tanaman Garcinia porrecta dan Garcinia forbesii yang dikoleksi di Laboratorium Mikrobiologi dan Bioteknologi Departemen Farmasi Universitas Indonesia. Lima galur isolat yang telah terbukti menunjukkan bioaktivitasnya sebagai antimikroba dan antioksidan diidentifikasi secara molekuler menggunakan profil sekuen DNA yang berasal dari gen penyandi ribosomal RNA. Identifikasi isolat kapang endofit dilakukan dengan metode Polymerase Chain Reaction (PCR) menggunakan primer NS1 dan NS4. Hasil amplifikasi dengan PCR selanjutnya dianalisis dengan sekuensing DNA yang kemudian dibandingkan dengan data pada GenBank dengan program BLAST (Basic Local Alignment Search Tools). Berdasarkan hasil analisis sekuens DNA, 5 isolat berhasil diidentifikasi. Empat isolat kapang endofit teridentifikasi sampai tingkat spesies yaitu: Penicillium purpurogenum, Zasmidium cellare, Colletothricum gloeosporioides, dan Nectria curta. Sedangkan satu isolat lain teridentifikasi sampai tingkat genus yaitu Pestalotiopsis sp. ABSTRACT The objective of this study was to identify the endophytic fungi isolated from Garcinia porrecta and Garcinia forbesii deposited in Laboratory of Microbiology and Biotechnology, Department of Pharmacy, University of Indonesia by using molecular tools. Five isolates of endophytic strains which were displayed the strongest antibacterial and antioxidant activities were identified based on ribosomal RNA (rRNA) gene sequence analysis. The Polymerase Chain Reaction (PCR) was used to identify these endophytic fungi by using primer NS1 and NS4. Amplicon or PCR product was analyzed by sequencing method to determine the DNA sequence. The result of sequencing DNA then compared with GenBank’s database using Basic Local Alignment Search Tool (BLAST). The results revealed that five isolates of endophytic strains had been successfully identified by this method. Four of them were identified up to the level of species as Penicillium purpurogenum, Zasmidium cellare, Colletothricum gloeosporioides, and Nectria curta, while the other one was identified up to the level of genus namely Pestaloptiosis sp.
MOLECULAR IDENTIFICATION OF ENDOPHYTIC FUNGI ISOLATED FROM Garcinia porrecta AND Garcinia forbesii Radji, Maksum; Nugraheni, Femi Arifah; Sumiati, Atiek
Jurnal Farmasi Indonesia Vol 4, No 4 (2009)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v4i4.22

Abstract

Penelitian ini dilakukan untuk mengidentifikasi isolat-isolat kapang endofit yang telah diisolasi dari tanaman Garcinia porrecta dan Garcinia forbesii yang dikoleksi di Laboratorium Mikrobiologi dan Bioteknologi Departemen Farmasi Universitas Indonesia. Lima galur isolat yang telah terbukti menunjukkan bioaktivitasnya sebagai antimikroba dan antioksidan diidentifikasi secara molekuler menggunakan profil sekuen DNA yang berasal dari gen penyandi ribosomal RNA. Identifikasi isolat kapang endofit dilakukan dengan metode Polymerase Chain Reaction (PCR) menggunakan primer NS1 dan NS4. Hasil amplifikasi dengan PCR selanjutnya dianalisis dengan sekuensing DNA yang kemudian dibandingkan dengan data pada GenBank dengan program BLAST (Basic Local Alignment Search Tools). Berdasarkan hasil analisis sekuens DNA, 5 isolat berhasil diidentifikasi. Empat isolat kapang endofit teridentifikasi sampai tingkat spesies yaitu: Penicillium purpurogenum, Zasmidium cellare, Colletothricum gloeosporioides, dan Nectria curta. Sedangkan satu isolat lain teridentifikasi sampai tingkat genus yaitu Pestalotiopsis sp. ABSTRACT The objective of this study was to identify the endophytic fungi isolated from Garcinia porrecta and Garcinia forbesii deposited in Laboratory of Microbiology and Biotechnology, Department of Pharmacy, University of Indonesia by using molecular tools. Five isolates of endophytic strains which were displayed the strongest antibacterial and antioxidant activities were identified based on ribosomal RNA (rRNA) gene sequence analysis. The Polymerase Chain Reaction (PCR) was used to identify these endophytic fungi by using primer NS1 and NS4. Amplicon or PCR product was analyzed by sequencing method to determine the DNA sequence. The result of sequencing DNA then compared with GenBankâ??s database using Basic Local Alignment Search Tool (BLAST). The results revealed that five isolates of endophytic strains had been successfully identified by this method. Four of them were identified up to the level of species as Penicillium purpurogenum, Zasmidium cellare, Colletothricum gloeosporioides, and Nectria curta, while the other one was identified up to the level of genus namely Pestaloptiosis sp.
Uji Aktivitas Antimikroba dan Uji Sitotoksik Ekstrak Etanol Akar Tanaman Akar Kucing (Acalypha indica Linn), Daging Buah Mahkota Dewa (Phaleria macrocarpa (Sheff) Boerl) dan Sari Buah Merah (Pandanus conoideus Lam) Radji, Maksum; Sari, Ratna Chandra; Sumiati, Atiek
Majalah Ilmu Kefarmasian Vol. 5, No. 1
Publisher : UI Scholars Hub

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Abstract

The antimicrobial activity and cytotoxic effect of ethanol extract of Acalypha indica Linn, Phaleria macrocarpa (Sheff) Boerl and Pandanus conoideus Lam, had been carried out. The results of the research showed that those ethanol extracts had antimi-crobial activities against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosae ATCC 27853 and Candida albicans. The determi-nation of cytotoxic effects of those ethanol extracts by brine shrimp lethality test (BSLT) using Artemia salina Leach showed that LC50 of Acalypha indica Linn, Phaleria macrocarpa (Sheff) Boerl and Pandanus conoideus Lam were 1,279 ug/ml, 0.123 ug/ml and 0.054 ug/ml respectively.
Uji Mutagenisitas dan Anti Kanker Ekstrak Aseton Dan N-Heksana Dari Kulit Batang Sesoot (Garcinia picrorrhiza Miq.) Radji, Maksum; Sumiati, Atiek; Indani, Nuning
Majalah Ilmu Kefarmasian Vol. 1, No. 2
Publisher : UI Scholars Hub

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Abstract

Mutagenicity and anti-mutagenicity of the acetone and n-hexane extracts of Garcinia picrorrhiza Miq.bark have been studied by Ames method using Salmonella typhimurium TA 97, TA 98 TA 100, TA 102, dan Escherichia coli WP2. The results showed that the two extracts had a positive effect. It can be concluded that the acetone and n-hexane extracts of Garcinia picrorrhiza Miq. bark have anticancer activity.
DETEKSI CEPAT BAKTERI Escherichia coli DALAM SAMPEL AIR DENGAN METODE POLYMERASE CHAIN REACTION MENGGUNAKAN PRIMER 16E1 DAN 16E2 Radji, Maksum; Puspaningrum, Anglia; Sumiati, Atiek
Makara Journal of Science Vol. 14, No. 1
Publisher : UI Scholars Hub

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Abstract

Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction Using 16E1 and 16E2 Primers. The detection of Escherichia coli in various water samples by Polymerase Chain Reaction method using 16E1 and 16E2 primers has been carried out. It was found that four out of ten samples were detected Escherichia coli and revealed the presence of the amplified product of the size 584 base pairs. Confirmation test of PCR result was also done using the conventional culturing method. The results of this study showed that the PCR assay was specific and faster than the conventional culturing method for detection of Escherichia coli.
Co-Authors Maksum Radji Nugraheni, Femi Arifah Nugraheni, Femi Arifah Nuning Indani, Nuning Puspaningrum, Anglia Ratna Chandra Sari