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All Journal Jurnal Biosains Pascasarjana Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Advances in Tropical Biodiversity and Environmental Sciences
Woro Hastuti Satyantini
Airlangga University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Bioencapsulation Artemia with Bacillus subtilis and Sodium Alginate on Total Hemocyte and Survival of Litopenaeus vannamei Infected with Vibrio parahaemolyticus Anggraini Widihastuti; Woro Hastuti Satyantini
Jurnal Biosains Pascasarjana Vol. 25 No. 1 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i1.2023.8-14

Abstract

Disease control in shrimp culture is mostly done by using probiotics such as B. subtilis. Immunostimulants are also widely used to improve the white shrimp immune system. One of the immunostimulants that can be used is sodium alginate. The purpose of this study was to determine the effect of bioencapsulation of Artemia with the combination of B. subtilis and sodium alginate on total hemocytes and the survival of white shrimp infected with V. parahaemolyticus. This study used a completely randomized design (CRD) with 5 treatments and 4 replications. Hemocyte total and survival were measured at H0, H7 after bioencapsulation, H9 one day post-infection, and H16 seven days post-infection. The results showed that the total hemocytes H7 and H9 in treatment P3 were significantly different (p<0.05) against all treatments, while the survival of white shrimp in H9, P1 and P3 treatment was not significantly different (p>0.05), to the treatment of K- and P2. The conclusion of this study was that the bioencapsulation of Artemia with the combination of B. subtilis and sodium alginate could increase total hemocytes and the survival of PL white shrimp infected with V. parahaemolyticus.
Microbial Profile of Frozen Fisheries Products at UPT PMP2KP Surabaya Fadiya Furuujihim Rohsarifuddin; Yolandha Sephiani Nurhafifah; Puspa Maharani; Kismiyati Kismiyati; Rahmah Hendriyati; Woro Hastuti Satyantini
Juvenil Vol 6, No 1: Februari (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i1.26337

Abstract

ABSTRACTMicrobial contamination of food products is one of the significant hazards in food safety because the nature of food-polluting bacteria is generally pathogenic.This research was conducted at the Microbiology Laboratorium in UPT PMP2KP Surabaya. Samples tested in this research including frozen fish, frozen shrimp, and frozen value added products with  microbiological parameters tested are Total Plate Count, Salmonella, and Escherichia coli. The results obtained in this research showed that 100% of 21 frozen fish and shrimp samples met SNI safety standards, namely 1 x 105  CFU/g for TPC, 3 MPN / g  for E. coli, and negative for Salmonella.Keywords: E. coli, Food Safety, Frozen Fisheries Product, Salmonella
Effectiveness of Algae Growth Promoter on Spesific Growth Rate and Growth Phase of Chaetoceros calcitrans Alfarisi Prafanda; Woro Hastuti Satyantini; Ahmad Shofy Mubarak
Advances in Tropical Biodiversity and Environmental Sciences Vol. 10 No. 2 (2026): June 2026
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2026.v10.i02.p08

Abstract

This research is motivated by efforts to provide high-quality microalgae as natural feed for shrimp hatchery activities. Researchers have used fertilizers with appropriate N/P ratios, including commercial fertilizers. In this study, the author examines the use of the commercial fertilizer Algae Growth Promoter (AGP) as fertilizer in C. calcitrans culture. Current fertilizer use still largely focuses on specific growth rate and increasing microalgal density, which is considered fast, and pays less attention to microalgal nutritional content. The study administered AGP fertilizer (80, 90, 100, 110, 120 ppm) and a control treatment, with 100 ppm modified Guillard fertilizer added, over a culture period from the exponential to the stationary phase. AGP fertilizer reached the stationary phase later, at the 108th observation hour. This is superior to the control treatment, which entered the stationary phase at 72 hours of culture. The control treatment reached the exponential phase earlier, with a td value of 20.29 hours, the shortest doubling time among all treatments. Observations of growth rates were also carried out, where the control treatment was found to have the best specific growth rate, with a µ value of 0.0343 per hour, while the treatment with AGP fertilizer had a specific growth rate with a µ value ranging from 0.0255 to 0.0279 per hour, and the td value ranged between 24.89 and 27.27 hours.
Co-Authors A. Shofy Mubarak Alfarisi Prafanda Anggraini Widihastuti Fadiya Furuujihim Rohsarifuddin Kismiyati , Puspa Maharani Rahmah Hendriyati Yolandha Sephiani Nurhafifah