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Angka Lempeng Total Dan Cemaran Bakteri Pada Daging Domba di pasar Tradisional Kampung Lalang Erika Triayu; Kurniawan Sinaga; Alfath Rusdhi
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 2 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.953 KB) | DOI: 10.36490/journal-jps.com.v6i2.74

Abstract

Meat is an important food ingredient in meeting nutritional needs. The purpose of this study was to detect total plate count and bacterial contamination in lamb meat. The research method used in this research is descriptive method, because this research describes the characteristics of a population or a phenomenon that is the object of research that is experimental. Observational data were analyzed using a descriptive method of 6 samples of lamb meat obtained from the traditional market in Kampung Lalang and found Escherichia coli and Salmonella sp. The highest TPC analysis values ​​were found in traders 2 and 3 samples 2, namely 6.45x106 cfu/g and 7.05x106 cfu/g and the lowest amount of contamination was found in traders 1 sample 2, namely 4.5x105 cfu/g. In accordance with the limit for Escherichia coli 1x 101 colonies/gram and the limit for Salmonella sp negative/25 gram. The results showed that 95% of the lamb meat samples were contaminated with Escherichia coli and Salmonella sp. This shows that the lamb meat sold in Kampung Lalalang traditional markets does not meet the Indonesian National Standard (SNI) or is unfit for consumption.
Deteksi Bakteri Patogen Pada Daging Ayam Di Pasar Tradisional Hamparan Perak Nuraini Sihotang; Kurniawan Sinaga; Alfath Rusdhi
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 2 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.863 KB) | DOI: 10.36490/journal-jps.com.v6i2.76

Abstract

Chicken meat is a source of animal protein for humans. The aim of this study was to detect pathogenic bacteria in chicken meat sold at the Silver Plain traditional market. The method used in this study is descriptive because research that describes the characteristics of a population or a phenomenon that is the object of research is trial and error. From the observational data analyzed descriptively as many as 10 samples from 10 traders obtained from the silver overlay market found the presence of Salmonella sp and Escherichia coli bacteria. The results showed that the highest number of colonies was found in trader 10, namely 3.8x106 cfu/g and the lowest in trader 5, namely 3.9x104 cfu/g, this was not in accordance with the maximum limit of microbial contamination in foodstuffs (fresh chicken meat) according to the National Standard. Indonesia (SNI) 7388:2009 namely TPC 1 x 106 colonies/gram. The results also showed that 70% of the chicken meat samples were contaminated with Salmonella sp bacteria, and 60% of the samples were contaminated with Escherichia coli bacteria. This shows that the quality of chicken meat sold in the Silver Plain traditional market does not meet the Indonesian National Standard (SNI) 7388:2009 Escherichia coli 1 x 101 colonies/gram, and Salmonella sp negative/25gram
Uji Cemaran Bakteri E. coli dan Salmonella sp. Pada Daging Sapi Di Pasar Tradisional Kecamatan Hamparan Perak Silvi Eka Putri; Kurniawan Sinaga; Alfath Rusdhi
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 2 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.66 KB) | DOI: 10.36490/journal-jps.com.v6i2.78

Abstract

Indonesian people prefer beef as a source of meat. More production and focus on high quality beef to meet the increasing demand for beef. The purpose of this study was to ascertain whether beef at the local market in Hamparan Perak District was contaminated with Salmonella sp. and E. coli. This research was conducted in January and February 2023 and is a descriptive study. Five samples of beef sold at the Klumpang Inpres Market, Hamparan Perak District, were used in this study. The findings revealed 18 bacterial isolates, most of which were round with intact edges, most of which were flat in height, and almost all of which were white. Seller 3 has the largest total number of microorganisms with a sample number of PS3 4.8 106 CFU / g, while seller 5 has the lowest number of PS5 samples 1.3 106 CFU / g. Contamination test results for Salmonella sp. and E.coli showed that all meat samples tested positive for Salmonella sp. and E. coli. A total of 4 isolates of gram-positive bacteria with purple color were found in the findings of bacterial staining, while there were 14 isolates of gram-negative bacteria with red and pink colors.
PENGARUH EXERCISE TERHADAP KULITAS SPERMATOZOA PADA SAPI SIMENTAL Purwo Siswoyo; Alfath Rusdhi; Agus Suleman
Jurnal Ilmu Teknologi Ternak Unggul (JITTU) Vol 1 No 2 (2022): Volume 1 Nomor 2, Desember 2022
Publisher : Yayasan Amanah Nur Aman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58432/jittu.v1i2.848

Abstract

ABSTRAK Penelitian pengaruh exercise terhadap kualitas spermatozoa pada sapi simmental dilaksanakan di Laboratorium UPTD Inseminasi Buatan Dinas Ketahanan Pangan dan Peternakan Provinsi Sumatera Utara. Tujuan penelitian ini adalah untuk mengetahui seberapa besar pengaruh exercise terhadap kualitas spermatozoa sapi simmental. Penelitian ini mengunakan tiga ekor sapi jantan yang berumur 2-3 tahun. Analisis data yang digunakan pada penelitian ini adalah rancangan acak lengkap (RAL) dengan 4 perlakuan dan 5 ulangan. P0: spermatozoa ditampung tanpa exercise. P1: spermatozoa ditampung dengan exercise 5 menit. P2: spermatozoa ditampung dengan exercise 10 menit. P3: spermatozoa ditampung dengan exercise 15 menit yang dilakukan sebelum penampungan. Parameter yang diamati dalam penelitian ini meliputi gerak massa, gerak individu, konsentrasi, viabilitas (persentase hidup – mati). Hasil penelitian menunjukkan bahwa pemeriksaan adalah volume semen rata-rata 4,2 ml dengan rataan 2,5 – 7,5, warna putih susu dan krem, pH 7 konsistensi sedang-kental. Rataan gerak massa 2,35 ± 0,25. Gerak individu 2,00 ± 0,00, konsentrasi 1.946 ± 179,20, viabilitas 77,11 ± 3,14 dapat disimpulkan bahwa perlakuan exercise tidak berpengaruh nyata terhadap kualitas spermatozoa sapi simmental. Kata kunci: exercise, spermatozoa, sapi simmental. ABSTRACT The study of the effect of exercise on the quality of spermatozoa in simmental cattle was carried out in the UPTD Laboratory of Artificial Insemination of the Food and Animal Husbandry Service of North Sumatra Province. The purpose of this study was to find out how much influence exercise has on the quality of simmental cow spermatozoa. This study used three bulls aged 2-3 years. The data analysis used in this study was a completely randomized design (CRD) with 4 treatments and 5 replications. P0: spermatozoa are collected without exercise. P1: The spermatozoa is accommodated by a 5-minute exercise. P2: spermatozoa are accommodated by a 10-minute exercise. P3: Spermatozoa are collected by a 15-minute exercise carried out before the shelter. The parameters observed in this study included mass motion, individual motion, concentration, viability (percentage of life - death). The results showed that the examination was an average cement volume of 4.2 ml with an average of 2.5 - 7.5, milky white and creamy, pH 7 medium-thick consistency. Average mass motion 2.35 ± 0.25. Individual movements of 2.00 ± 0.00, concentration of 1.946 ± 179.20, viability of 77.11 ± 3.14 can be concluded that exercise treatment did not significantly affect the quality of simmental cow spermatozoa. Keywords: exercise, spermatozoa, simmental cattle.
PEMANFAATAN TEPUNG DAUN SIRSAK (ANNONA MURICATA L) SEBAGAI FEED ADDITIVE TERHADAP PERFORMANCE AYAM JOPER Ryan Purade; Alfath Rusdhi; Purwo Siswoyo
Jurnal Ilmu Teknologi Ternak Unggul (JITTU) Vol 1 No 2 (2022): Volume 1 Nomor 2, Desember 2022
Publisher : Yayasan Amanah Nur Aman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58432/jittu.v1i2.849

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung daun sirsak (annona moricata l) sebagai feed additive terhadap performance ayam joper dilaksanakan di Jalan Amal Desa Tanjung Anom, Kecamatan Pancur Batu, Kabupaten Deli Serdang, Provinsi Sumatera Utara. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) 4 perlakuan dan 5 ulangan. Perlakuan yang diberikan yaitu P0 = (Kontrol), P1 = pakan komersil + tepung daun sirsak 50 gram per kilogram pakan, P2 = pakan komersil + tepung daun sirsak 100 gram per kilogram pakan, P3 = pakan komersil + tepung daun sirsak 150 gram per kilogram pakan. Hasil penelitian ini menunjukkan bahwa pemberian tepung daun sirsak tidak berpengaruh nyata terhadap pertambahan bobot badan (P>0.05) pakan kontrol (P0) dengan nilai tertinggi sebesar 13,59 (gr/ekor/hari) kemudian konsumsi pakan (P>0.05) P0 dengan nilai terendah sebesar 38,89 (gr/ekor/hari), dan konversi pakan (P>0.05) terendah pakan kontrol (P0) sebesar 2,89. Kata Kunci : Ayam Jawa Super, Pertambahan Bobot Badan, Konsumsi Pakan, Konversi Pakan. ABSTRACT This study aims to determine the effect of giving soursop leaf flour (annona moricata l) as a feed additive to the performance of hens. It was carried out at Jalan Amal Desa Tanjung Anom, Pancur Batu District, Deli Serdang Regency, North Sumatra Province. The method used was a completely randomized design (CRD) with 4 treatments and 5 replications. The treatments given were P0 = (Control), P1 = commercial feed + 50 grams of soursop leaf flour per kilogram of feed, P2 = commercial feed + 100 grams of soursop leaf flour per kilogram of feed, P3 = commercial feed + 150 grams of soursop leaf flour per kilogram feed. The results of this study indicate that the provision of soursop leaf flour has no significant effect on body weight gain (P> 0.05) control feed (P0) with the highest value of 13.59 (gr / head / day) then feed consumption (P>0.05) P0 with The lowest value was 38,89 (gr / head / day), and the lowest feed conversion (P> 0.05) was control feed (P0) was 2.89. Keywords: Super Javanese Chicken, Weight Gain, Feed Consumption, Feed Conversion.
Deteksi Bakteri Proteolitik Dari Ileum Gallus Gallus Sebagai Kandidat Agen Probiotik Pakan Fermentasi Unggas Tengku Gilang Pradana; Alfath Rusdhi; Indah Pratiwi Purba
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.261

Abstract

The ileum is part of the small intestine that functions as the absorption of food and for the growth and development of bacteria to degrade feed, such as lactic acid bacteria. The purpose of this study was to explore and detect proteolytic bacteria from the ileum of Gallus gallus as a candidate probiotic agent. The research method is a selection of proteolytic bacteria, catalase, and motility. The results showed that the average proteolytic index in isolates GP sp. 4, GP sp.3, and GP sp. 1 were 1.533, 1.531, and 1.500. Nine isolates were gram-positive and one gram-negative (GP sp. 3). The ten isolates could hydrolyze hydrogen peroxide, as indicated by the formation of air bubbles. The motility test showed that eight isolates were motile and the other two isolates were non-motile (GP sp.8 and GP sp.10). The ten proteolytic bacterial isolates obtained did not have the potential to be used as probiotic agents for fermented poultry feed because low proteolytic index value.
Uji Kualitas Kimia Kefir Dari Imbangan Susu Kambing Dan Susu Sapi Dengan Lama Fermentasi Yang Berbeda Alfath Rusdhi; Purwo Siswoyo; Tiara Adelia
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.284

Abstract

The research aims to study the quality of kefir chemistry from the balance of goat's milk and cow's milk with different fermentation periods. Research uses experimental methods with a complete random design of factorial patterns. The first factor is the type of milk (cow milk 100%, goat milk 50% + milk cow 50%, and goat milk 100%), the second factor is long permentation (24, 36 and 48 hours). The results showed the type of milk, the length of the permentation and the interaction of real effect on protein levels, fats, alcohol, total dissolved solids and total lactic acid. Kefir quality is a balanced high protein, low in fat and alcohol found in goat's milk kefir with fermentation for 60 hours, where the amount of protein is 4.16%, fat 4.27%, and alcohol 0.59%, Total dissolved solids 5.5667% and Total lactic acid is 0.7871%.
EFEKTIVITAS PENGGUNAAN PROBIO GAP 1 DALAM RANSUM TERHADAP PERTUMBUHAN AYAM BROILER Muhammad Sadiqul Amin; Tengku Gilang Pradana; Alfath Rusdhi
Jurnal Ilmu Teknologi Ternak Unggul (JITTU) Vol 2 No 2 (2023): JURNAL ILMU TEKNOLOGI TERNAK UNGGUL
Publisher : Yayasan Amanah Nur Aman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58432/jittu.v2i2.1015

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh Efektivitas Penggunaan Probio GAP 1 dalam ransum terhadap performans ayam broiler serta mengevaluasi penggunaan probiotik yang paling sesuai pada ransum yang paling tepat untuk menghasilkan performance yang baik berdasarkan perlakuan dan ulangan yang dilakukan. Analisis karakteristik performance ayam broiler dilakukan di Universitas Pembangunan Panca Budi. Penelitian dilaksanakan selama 1 (satu) bulan yaitu Januari sampai dengan Februari 2023. Metode yang digunakan pada penelitian ini adalah metode eksperimen. Rancangan yang digunakan dalam penelitian ini Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 3 perlakuan dan 5 ulangan. Perlakuan yang digunakan adalah P0 (ransum 100%), P1 (10 ml untuk 1 kg ransum), P2 (15 ml untuk 1 kg ransum), P3 (20 ml untuk 1 kg ransum). Data dianalisis dengan sidik ragam dengan parameter konsumsi ransum, pertambahan bobot badan, konversi ransum. Data yang diperoleh dianalisis secara sidik ragam dari RAL yang menunjukkan bahwa pemberian probiotik GAP 1 berpengaruh nyata terhadap konsumsi pakan, pertambahan bobot badan (PBB) dan konversi ransum (P<0,05) dengan menggunakan pakan perlakuan P3 (20 ml untuk 1 kg ransum). Kata Kunci: Broiler,Performa,Probiotik. ABSTRACT This study aims to determine the effect of the effectiveness of the use of Probio GAP 1 in rations on the performance of broiler chickens and evaluate the use of the most suitable probiotics in the most appropriate ration to produce good performance based on the treatment and tests carried out. Analysis of broiler chicken performance characteristics was carried out in Universitas Pembangunan Panca Budi. The research was carried out for 1 (one) month, namely January to February 2023. The method used in this study is an experimental methode. The design used in this study was a non- factorial Complete Randomized Design (RAL) consisting of 3 treatments and 5 tests. The treatment used is as follows P0 (ration 100%), P1 (10 ml for 1 kg ration), P2 (15 ml for 1 kg ration), P3 (20 ml for 1 kg ration). The data were analyzed by fingerprinting with the parameters of ration consumption, body weight gain, ration conversion. The data obtained were analyzed in a fingerprint from RAL which showed that the administration of GAP 1 probiotics had a significant effect on feed consumption, body weight gain (PBB) and ration conversion (P<0.05) using P3 treated feed (20 ml for 1 kg of ration). Keywords: Broilers, Performance, Probiotics.