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All Journal SAINS TANAH - Journal of Soil Science and Agroclimatology AgriHealth: Journal of Agri-food, Nutrition and Public Health
Sumani Sumani
Department of Soil Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta
Agriculture, Biological Sciences & Forestry Education Environmental Science Health Professions Public Health

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Identification and pesticide degradation test of bacterial consortium of contaminated soil Retno Rosariastuti; Yulia Rahmawati; Sumani Sumani; Sri Hartati
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 20, No 1 (2023): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v20i1.62920

Abstract

Excessive use of pesticides in the process of cultivating shallots results in contamination of the soil. Indigenous bacteria in soil that contaminated with the chlorpyrifos pesticide suspected could used as bioremediation agent of soil contaminated with chlorpyrifos pesticide. The purpose of this study was to identify and obtain a consortium of bacteria capable in degrading pesticides on shallot fields contaminated with the pesticide chlorpyrifos. The method used in this research is the bacteria isolation using soil extract, morphological identification, molecullar identification using Next Generation Sequencing (NGS), analysis of bacterial diversity index and consortium bacterial degradation test in order to reduce levels of the chlorpyrifos pesticide. The results of this study found 16 isolates of bacteria at each study site that were resistant to 100ppm of the pesticide chlorpyrifos so that these isolates were chosen to be used as a consortium of bacteria. Molecular identification of the bacterial consortium showed that there were 10 genera consisting of Cutibacterium, Streptomyces, Staphylococcus, Ensifer, Ochrobactrum, Achromobacter, Escherichia shigella, Klebsiella, Acinetobacter, and Pseudomonas. The bacterial diversity index in shallot soils reached 2,040 and 1,467 on forest soils The reduction efficiency of the bacterial consortium using the method of growing cells and supernatant were 94.48% and 98.88%, respectively.
Assessment of Land Quality for Siamese Orange (Citrus nobilis var. microcarpa) Development in Pacitan Regency, Indonesia Rahayu Rahayu; Supriyadi Supriyadi; Sumani Sumani; Aktavia Herawati; Kurnia Mukti Dewi; Yang Geun Mo; Eun Ji Bae
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 5, No 1 (2024): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v5i1.80729

Abstract

Siamese oranges are one of the most widely developed horticultural crops in Indonesia. Arjosari and Ngadirojo are some of the sub-districts that produce Siamese orange in Pacitan Regency. One of the factors that can affect the development of citrus is land quality. This study aims to obtain land quality index data and improvement efforts for developing Siamese orange. Soil samples were taken from 25 land mapping units (LMUs) with 87 sample points. The soil sampling points were determined based on area or sampling density. Data analysis used for soil quality assessment includes correlation, principal component analysis (PCA), minimum data set (MDS), and calculation of climate quality index. The parameters used in this study are bulk density, porosity, moisture content, texture, pH, electrical conductivity (EC), cation exchange capacity (CEC), base saturation, total N, available P, available K, soil organic carbon (SOC), microbial biomass carbon (MBC), evapotranspiration, temperature, and solar radiation. Based on the results of soil quality analysis in the study area, it has a value range of 0.28 to 0.37, including low class, while the climate quality index is 1.39 with moderate class. The land quality index has a very low to low class with a value of 0.39 to 0.51. Intrinsic, extrinsic, and anthropogenic factors can influence soil quality. The study area’s limiting factors for developing Siamese oranges are EC, total N, and available P, which can be improved by making furrow ponds, adding organic fertilizer, using urea fertilizer, and SP36 according to the dosage.
Co-Authors AKTAVIA HERAWATI Eun Ji Bae Kurnia Mukti Dewi Rahayu Rahayu Retno Rosariastuti Sri Hartati Supriyadi Supriyadi Yang Geun Mo Yulia Rahmawati