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Nancy D F K Foeh
Universitas Nusa Cendana
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health Veterinary

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Motilitas dan Viabilitas Spermatozoa Babi dalam Pengencer Air Kelapa-Kuning Telur Ayam Kampung Crecentia Aprilinda Butta; Cynthia Dewi Gaina; Nancy D F K Foeh
Jurnal Veteriner Nusantara Vol 4 No 1 (2021): Februari 2021
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v4i1.6033

Abstract

The aims of this study is to determine the effect and the best combination of coconut water (CW) and native chicken egg yolk (NCEY) as extender with water jacket and non-water jacket method in preserving of boar semen. Semen was collected from sexualy mature boar 2-4 years of age. A good sample of semen will have motility above 70%, abnormality below 20% and spermatozoa consentration above 200 x 106 sel/mL. This study was designed with completly randomized design and faktorial patern with 2 controled group, 6 treated group, and 4 repitition. Controled group consist of fresh semen without extender. K0 was preserved with non-water jacket method, and K1 was preserved with water jacket method. Treated group were P1 (75% CW + 25% NCEY), P2 (85% CW + 15% NCEY), P3 (95% CW + 5% NCEY) that preserved with non-water jacket method, and P4 (75% CW + 25% NCEY), P5 (85% CW + 15% NCEY), P6 (95% CW + 5% NCEY) preserved with water jacket method. Motility and viability of spermatozoa were evaluated every 2 hours until motility value has reached 40%. The result shows that P3 and P5 is the best combination for preserving semen (32 hours). Water jacket method is the better option for preserving boar semen. P3 is the least in preserving boar semen.
Pengaruh Ukuran Testis Terhadap Kualitas Semen Babi Landrace Dan Babi Duroc Yoha Raldi Nadja; Cynthia Dewi Gaina; Nancy D F K Foeh
Jurnal Veteriner Nusantara Vol 4 No 1 (2021): Februari 2021
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v4i1.6036

Abstract

The objective of this study was to determine the effect of testicular size on spermatozoa quality from landrace pigs and duroc pigs that had mature with age ranging 2-4 years. The macroscopic and microscopic data obtained from this study will be analyzed descriptively and for the effect of testicular size on spermatozoa quality tested using complete randomized design (RAL) using ANOVA. The results showed the average of the observations of landrace pig cement on macroscopic basis as follows: volume obtained 236.7 ml, consistency dilute, milk white, pH 7 and has the smell of a pig. Based on the variance test, the average of spermatozoa is 82.5%, spermatozoa viability is 88.6%, 12.9% abnormality and spermatozoa concentration of 215 x 106 cells / ml. The mean size of testes of landrace pig and duroc pig is calculated using excel application and testicular area calculated using PxL formula. The results obtained were as follows: left testis length of landrace pork 12.66 ± 3cm, right testis length 14 ± 1cm, left testis pavement landrace 7.66 ± 2.081cm, right testis width 8 ± 0cm with left testis area and right testis of landrace pigs respectively 96.97cm and 112cm. Duroc pigs have a length of left and right testis respectively 16.66 ± 5.507cm and 13.66 ± 3.21cm, the left and right testis widths of 83 ± 2.020cm and 8.5 ± 1.5cm respectively and the size of the left and right testis respectively are 147.1cm and 116.11cm. So tu sum up the testicular size significantly affected the semen volume produced by a pig and the size of the testes had no significant effect on motility, viability, concentration, abnormality, color, consistency, pH and odor.
Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) Sebagai Antioksidan Dalam Pengencer Semen Babi Landrace Berbasis Air Buah Lontar Maria Gisela Goncalves Gena; Nancy D F K Foeh; Cynthia Dewi Gaina
Jurnal Veteriner Nusantara Vol 4 No 1 (2021): Februari 2021
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v4i1.6041

Abstract

This study aims to determine the antioxidant effect of the ethanol extract of the leaves of Moringa (Moringa oleifera Lam.) In water diluent palm fruit of the quality of landrace pig spermatozoa. This study uses a Completely Randomized Design (CRD) with 5 treatments, 1 control dan 4 repetitions and observation of the motility and viability of spermatozoa performed every 2 hours until a decline in the percentage motility of at least 40%. The cement used in the Laboratory of Technical Services Unit for Breeding and Forage at Tarus, Kupang. Escrow done every two weeks from the male landrace pigs who have experienced sexual maturity. Landrace pig semen storage results were evaluated macroscopically and microscopically. Good quality cement had ≥70% motility, concentration ≥200x106 sel spermatozoa/ml and abnormalities of ≤20%. Cement is added to the water diluent palm fruit then added with antioxidants. Antioxidants should be added during the storage process, sperm metabolic activity produces free radicals that can degrade the quality of spermatozoa. Moringa leaves (Moringa oleifera Lam.) Is one of the antioxidants that can be used to counteract free radicals. Moringa leaves (Moringa oleifera Lam.) Is added to the water diluent palm fruit with graded doses are 1% (P1), 2% (P2), 3% (P3), 4% (P4) and 5% (P5). The results of this study indicate a dose of 1% (P1) is the best dosage to maintain the quality of the landrace pig spermatozoa of 66.25 ± 2.39 motility and viability of spermatozoa sebesar 78.00% ± 1.82%, which is stored at room temperature 22º C.
Kualitas Spermatozoa Babi dalam Pengencer Air Buah Lontar dan Kuning `Telur Ayam Kampung dengan Metode Penyimpanan yang Berbeda Maria Selviana Bebbe Bei; Nancy D F K Foeh; Cynthia Dewi Gaina
Jurnal Veteriner Nusantara Vol 4 No 1 (2021): Februari 2021
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v4i1.6046

Abstract

The aim of this study is to find the best concentration ratio of native chicken egg yolk (NCEY) and lontar water (LW) in order to preserve landrace boar semen, with water jacket and non-water jacket method, at 5°C and to find how long can it preserve the semen. Fresh semen was collected from Landrace boar 2-4 years old age. Semen sample is categorized apropriate to be extended if >70% sperm motility, >200x106 cells/ mL, and >20% sperm abnormality. This study was designed with compeltely ranomized design and faktorial patern with 2 controled group, 6 treated group, and 4 repitition. Controled group consist of fresh semen without extender. K0 was preserved with non-water jacket method, and K1 was preserved with water jacket method. Controled group were P1 (95% LW + 5% NCEY), P2 (85% LW + 15% NCEY), P3 (75% LW + 25% NCEY) that preserved with water jacket method, and P4 (95% LW + 5% NCEY), P5 (85% LW + 15% NCEY), P6 (75% LW + 25% NCEY) preserved with non-water jacket method. Sperm motility and viablity were evaluated every 2 hours until motility value has reached 40%. The result shows that P2 is the best concentration to preserve sperm motility (26 hours, 45.00±0.00%) and viability (30 hours, 51.00±0.40%).
Co-Authors Crecentia Aprilinda Butta Gaina, Cynthia Dewi Maria Gisela Goncalves Gena Maria Selviana Bebbe Bei Yoha Raldi Nadja