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Agus Saputra
Universitas Nusa Cendana
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Uji Daya Anthelmintik Ekstrak Etanol Biji Pepaya (Carica papaya) Terhadap Cacing Ascaris suum Secara In Vitro Olivia Maria Ujan; Agus Saputra; Aji WINARSO
Jurnal Veteriner Nusantara Vol 4 No 1 (2021): Februari 2021
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v4i1.6045

Abstract

The purpose of this study was to determine the anthelmintic test of ethanol extract of papaya seeds on Ascaris suum worms in vitro and to find out the compounds contained therein based on phytochemical tests. The materials used were papaya seeds (Carica papaya), Ascaris suum worms, 70% ethanol, 0,9% NaCl, and Pyrantel pamoate. Extraction of papaya seeds was done by maceration method using 70% ethanol solvent. Identification of Ascaris suum worms was carried out based on the morphological characteristics of the worm (female/male, length, and diameter). Testing of ethanol extract of papaya seeds was carried out in 4 groups consisting of three treatment groups and one control group. Three treatment groups used ethanol extract of papaya seeds with a concentration of 2,5%, 5%, 7,5%, and the control group using pyrantel pamoate 7,5%. Potential of ethanol extract of papaya seeds was seen from the time needed to kill Ascaris suum worms. Where the ethanol extract of papaya seeds was 2,5% with an average mortality time of 5,96 hours, a concentration of 5% with a mean of 3,98 hours, a concentration of 7,5% with a mean of 2,52 hours and a control group with a mean time of death of 2,18 hours. The results showed that there was a statistically significant effect (P<0,05) on P1 and P2 and there was no significant effect (P>0,05) on P3. It can be concluded that immersion using pyrantel 7,5% is still higher than P1 and P2 and immersion using pyrantel 7,5% has the same anthelmintic effect as P3. In the phytochemical test, ethanol extract of positive papaya seeds contained saponin, tannin, alkaloid, phenol, flavonoid and triterpenoid.
In Silico Study of Bioactive Compounds from Faloak Stem Bark (Sterculia quadrifida R. Br.) Against Estrogen Receptor Alpha as Potential Breast Anticancer Agents Maria Wihelmina; Putri Pandarangga; Agus Saputra
Jurnal Sehat Indonesia (JUSINDO) Vol. 8 No. 2 (2026): Jurnal Sehat Indonesia (JUSINDO)
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/-.v8i2.556

Abstract

Estrogen receptor alpha (ERα) is an important therapeutic target in hormone-dependent breast cancer, prompting the investigation of natural compounds with potential receptor-binding activity. This study aimed to evaluate the binding affinities and molecular interaction profiles of six bioactive compounds reported in Faloak (Sterculia quadrifida R. Br.) stem bark against ERα. Ligand structures were retrieved from PubChem, while the three-dimensional structure of ERα was obtained from the Protein Data Bank (PDB ID: 3ERT). Molecular docking was performed using PyRx version 0.8, and selected binding poses were analyzed using BIOVIA Discovery Studio Visualizer. The co-crystallized ligand, 4-hydroxytamoxifen, was used as the reference ligand. The docking scores of the tested compounds ranged from −7.1 to −8.6 kcal/mol, compared with −9.5 kcal/mol for 4-hydroxytamoxifen. Catechin-4-ol 3-O-α-L-rhamnopyranoside exhibited the most favorable docking score among the tested compounds and formed interactions with Glu353, Leu387, Met343, Leu525, Met421, Phe404, and Leu391. The observed hydrogen-bonding and hydrophobic interactions showed similarities to the interaction profile of the reference ligand, supporting the prioritization of this compound for further investigation. These findings identify catechin-4-ol 3-O-α-L-rhamnopyranoside as a promising computational candidate for further ERα-targeted research. However, molecular docking alone cannot establish receptor antagonistic activity or anticancer efficacy. Further molecular dynamics simulations and experimental studies are required to evaluate binding stability, biological activity, safety, and potential therapeutic relevance in breast cancer.
Co-Authors Aji Winarso Maria Wihelmina Olivia Maria Ujan