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Wahyu Widoretno
Biology Departement, Faculty of Sciences, Brawijaya University
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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The Effect of Explant Types and Kinetin Concentration on In Vitro Callus Induction in Vetiveria zizanioides (L.) Nash Arbaul Fauziah; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1001.506 KB) | DOI: 10.21776/ub.jels.2017.007.02.04

Abstract

The aims of this research were to evaluate the effect of explant types and several kinetin concentrations on in vitro induction and growth of callus vetiver (Vetiveria zizanioides (L.) Nash). Crown and tiller of vetiver were cultured on Murashige and Skoog’s (MS) media supplemented with combination of 2,4-D 0.75 ppm and several kinetin concentrations (0, 0.3, 0.5, 0.75, and 1) ppm. The induction and growth of callus were influenced by type of explant and concentration of kinetin. Formation and growth of callus on tiller explant were faster than crown explant. Callus on tiller explant were formed one week after culture, while callus from crown explant were formed at four weeks after culture. Callus growth on tiller explant also was better than crown explant. Eight weeks after culture, callus fresh weight from tiller explant was 0.35 ± 0.09 g, while callus fresh weight from crown explant was only 0.16 ± 0.08 g. The addition of kinetin in the medium combined with 2,4-D was able to increase callus growth and the optimum concentration of kinetin used was 0.5 ppm. The addition of kinetin more than 0.5 ppm in the medium decreased the callus fresh weight.Keywords: callus, crown, in vitro culture, tiller, Vetiveria zizanioides.
Co-Authors Arbaul Fauziah