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Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Munawarti, Aminatun; Taryono, Taryono; Semiarti, Endang; Sismindari, Sismindari
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Responses of Two Cocoa Varieties to Environmental Stress Conditions Fuadati, Ayu Zahrotul; Prastowo, Erwin; Munawarti, Aminatun; Arimarsetiowati, Rina
AGRIVITA Journal of Agricultural Science Vol 47, No 2 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i2.4425

Abstract

Environmental changes resulting from climate anomalies may have significant implications for the future of global cocoa sustainability. A study was conducted to investigate the responses of two different cocoa varieties, i.e., ICCRI 06H and ICCRI 08H, to various environmental conditions at the greenhouse scale. Two distinct factors, i.e., green-house microclimate and soil moisture level, were simulated in a split-split plot design. A glass box equipped with artificial light was installed to modify the ambient temperature and air humidity, providing two different conditions: inside (IB) and outside (OB) the glass box, assigned as the main plot. The performance of cocoa seedlings was tested as a subplot, and they were exposed to different soil moisture levels, i.e., 100% soil moisture (ETa1), 50% soil moisture (ETa2), and 25% soil moisture (ETa3), defined as sub-subplot. Data suggested a slight difference in daily air temperature and relative humidity fluctuations, in both plots, observed as a function of time. A higher response of the stomatal index and density, up to 20%, was found in the ETa3 treatment for ICCRI 08H. The stress simulation increased the ICCRI 08H proline by up to 36% and guaiacol peroxidase by 27% higher than ICCRI 06H.
Efek Skarifikasi Air Panas Dengan Variasi Suhu Dan Waktu Pada Perkecambahan Biji Kacang Hijau (Vigna radiata L.) Setiyanto, Afif Eka Rahma; Munawarti, Aminatun
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.12176

Abstract

This study aims to analyze the effect of initial temperature variation and hot water scarification time on the germination of mung bean seeds. Seeds measuring 0.2 ± 0.05 cm in size and dark green in color were scarified using various scarification treatments, namely control (without scarification) (P0), room temperature for 10 minutes (P1), room temperature for 60 minutes (P2), 35°C for 10 minutes (P3), 35°C for 60 minutes (P4), and 55°C for 10 minutes (P5), with a germination period of seven days. The experimental design used was a completely randomized design with four replications, each replication using 25 seeds. The parameters observed were germination percentage, germination rate index, germination rate, radicle length, and plumule length. The best germination percentage and germination speed index were produced by the control (P<0.05). The best germination rate was produced by the P3 temperature treatment. Radicle length and plumule length did not differ significantly (P<0.05) across all treatments. Hot water scarification with varying temperatures and times in this study did not induce better mung bean germination than the control.
Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin Nafika, Wirentyas; Munawarti, Aminatun
Biotropika: Journal of Tropical Biology Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.03.02

Abstract

Apple rootstock is a plant type used in grafting propagation. Research related to the response of morphogenesis in this plant has not been widely carried out. This study aims to examine the influence of auxin and cytokinin ratios on morphogenesis in leaf and petiole explants of rootstock apples, seeking optimal response modifications. A 2-factorial, completely randomized design (CRD) was utilized in the investigation, with variations in the types of explants and combinations of plant growth regulators (PGR) in the culture medium. There were 12 treatments with five repetitions. Explants of young apple leaf (third leaf) and petiole rootstock that had been sterilized and cut with a size of 5x5 mm for the leaf and 5 mm long for the petiole were inoculated into MS medium with cytokinin, BAP (1 ppm) combined with auxin, IBA (0, 0.1, 0.2, 0.3, 0.4, 0.5 ppm). Each culture bottle contains five explants. The results showed that the average leaf explant was capable of producing 34% callus. Meanwhile, petiole explants produced 4% callus. Callus began to form on leaf and petiole explants on the 26th and 42nd days after inoculation, respectively. Most calluses have a compact texture with green, yellowish-white, and brownish-green variations. The PGR combination with the highest percentage of callus production in leaf explants was BAP 1 ppm + IBA 0.4 ppm treatment, whereas the petiole was BAP 1 ppm + IBA 0.3 ppm treatment. Therefore, the combination of PGR with the best callus response in this study can be used as a reference in the development of apple tissue culture.