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All Journal Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Molekul: Jurnal Ilmiah Kimia
Kurratul Aini
UNIVERSITAS ISLAM NEGERI RADEN FATAH
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston) Kurratul Aini; Elfita Elfita; Hary Widjajanti; Arum Setiawan
Molekul Vol 18 No 1 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.1.5931

Abstract

Endophytic fungi isolated from medicinal plants have high diversity and the secondary metabolites produced have biological activity. Endophytic fungi isolated from medicinal plants have been the subject of many studies on their diversity and biological activity, one of which is jambu mawar (Syzygium jambos (L.) Alston). S. jambos has long been used as a traditional medicine to treat infections caused by pathogenic bacteria in many parts of the world, including South Sumatra. This study purposed to explore the diversity of endophytic fungi isolated from the branches of S. jambos, the antibacterial activity of endophytic fungi extracts, the determination of the structure of the compounds, and the activity of the active compounds of the selected endophytic fungi. Observations of the morphological characteristics of endophytic fungi were macroscopically and microscopically. The endophytic fungal extracts were then tested for antimicrobial activity against test bacteria Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Escherichia coli using the Kirby-Bauer paper disk diffusion method. Isolation of pure compounds using a gravity chromatography column, the determination of the structure of the compound is determined based on 1H-NMR, 13C-NMR, HMQC, and HMBC spectroscopy. Four endophytic fungi, code SJC1–4, were isolated from a branch of S. jambos. The results of macroscopic and microscopic morphological characterization showed three genera of Botryosphaeria, Trichothecium, and Aspergillus. The endophytic fungal isolate SJC1 exhibited strong activity against Gram-positive bacteria and moderate activity against Gram-negative bacteria, while SJC2–4 showed moderate activity against the four bacteria. Molecular identification of SJC1 revealed that it was Botryosphaeria mamane. Isolation of SJC1’s pure compound yielded compound 1, which was identified as 5-acetyl-6-hydroxy-3-methyl-2H-pyran-2-one. This compound is thought to have antibacterial properties.
Identifikasi Kualitas Air dan Pelatihan Teknik Penjernihan Air Sederhana di Desa Tanjung Menang, Kabupaten Ogan Komering Ilir Wicaksono Anggun; Noviyanto Noviyanto; Alfia Rahma Kurnia; Kurratul Aini; Yustina Hapida; Rian Oktiansyah; Amin Nurokhman; Ummi Hiras Habisukan
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 3 (2023): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.9.3.304-317

Abstract

Clean water is a basic need for society. However, the problem of clean water sources still occurs in the Ogan Komering Ilir (OKI) area of South Sumatra Province. The condition of the water being brownish and oily is an unresolved problem. One source that has the potential to disrupt water quality is the presence of factories around rivers. This community service activity aims to identify water quality and provide training to the community regarding river water management strategies and drilled wells as the primary water source for obtaining clean water, as well as increasing understanding and knowledge about clean water management with the Gravity-fed Filter Technique. The method of activities related to clean water quality is carried out in four stages, namely: 1) Location survey (cross-sectional), 2) Water sampling and laboratory analysis, 3) Education on water quality standards, and 4) Training on Gravity-fed Filter Techniques. The laboratory analysis showed that the water samples smelled fishy and were brownish and oily. In addition, the results of microbiological tests on water samples contained Coliform bacteria (sanitation indicator bacteria), which were outside the provisions of Minister of Health Decree No. 907/MENKES/SK/VII/2002 concerning clean water quality requirements. Through training activities using the Gravity-fed Filter technique, the pH and bacteria contained in the water can be reduced. Clean water management strategies using the Gravity-fed Filter technique can be used as a solution to improve clean water quality. Every society can apply the Gravity-fed Filter technique. Through this activity, society has managed water sources into clean water for daily needs.
Co-Authors Alfia Rahma Kurnia Amin Nurokhman Arum Setiawan Elfita Hary Widjajanti Noviyanto Noviyanto Rian Oktiansyah Ummi Hiras Habisukan Wicaksono Anggun Yustina Hapida