Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Journal of Veterinary and Animal Sciences Jurnal Veteriner
I Made Kardena
Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Udayana University
Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Distribution and Elimination of Lead in Rat (Rattus norvegicus) Tissues I Ketut Berata; Ni Nyoman Werdi Susari; I Made Kardena; Ida Bagus Oka Winaya; I Wayan Nico Fajar Gunawan
Journal of Veterinary and Animal Sciences Vol 6 No 2 (2023)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2023.v06.i02.p02

Abstract

This study aims to determine the distribution and elimination of lead levels in various tissues of rats (Rattus norvegicus). The study used 32 rats which were divided into 2 groups, namely the control group and the group given 2.00 ppm Pb-acetate. Treatment by administering Pbacetat is carried out orally every day for 30 days (phase 1). On day 31 (phase 1), 8 rats from each group were taken their blood plasma for measurement of lead levels. Measurement of lead levels was carried out using the atomic absorption spectrophotometry (AAS) method. Then the rats were necropsied and the liver, kidneys, spleen, lungs, intestines, heart muscle and brain tissue were taken for histopathological preparation. Histopathological preparations were made according to the hematoxylin eosin (HE) staining. The remaining 8 rats from each group were kept continuously for the next 30 days (phase 2), without giving Pb-acetate solution. This phase aims to determine the level of lead elimination from rat tissues. On day 61, all remaining rats were taken their blood for measurement of lead content. Then the rats were necropsied to take liver, kidney, spleen, lung, intestine, myocardium and brain tissues, the same as in phase 1.The histopathologically examination were categorized based on thehaemorrhage, inflammatory and necrotic lesions. The average of measurement result of lead content in the blood of rats in phase 1 was 0.27±0.06 ppm. Whereas the average of lead content in phase 2 was 0.12±0.03 ppm. This result showed significantly difference by variance of analize. Based on the tissue lesions, the liver, kidneys, spleen and lungs were the main tissues undergoing histopathological changes. Up to phase 2, the liver tissue still has lesions. It can be concluded that lead contamination in 30 days can significantly decrease in the next 30 days. Lead distribution can cause lesions in liver, kidney, spleen and lung tissues. But in phase 2, liver lesions were still found, indicating that liver tissue had the lowest elimination power compared to other tissues
Clinical, Pathological and Serological Identification of Newcastle Disease in Chicken in Kintamani and Susut District of Bangli, Bali Inda Sabrina Latifah; Gusti Ayu Yuniati Kencana; Ida Bagus Kade Suardana; Tjokorda Sari Nindhia; Romy Muhammad Dary Mufa; I Made Kardena
Jurnal Veteriner Vol. 27 No. 3 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Newcastle Disease (ND), caused by Avian Paramyxovirus serotype 1 (APMV-1), remains endemic in Indonesia and continues to threaten poultry production due to its high transmissibility and economic impact. This study aimed to identify ND through an integrated clinical, pathological, and serological approach in commercial and indigenous chickens from Susut and Kintamani Districts, Bangli Regency, Bali. A total of 32 clinically suspected chickens (16 per district) were examined by necropsy, virus isolation in embryonated chicken eggs, and confirmation using hemagglutination (HA) and hemagglutination inhibition (HI) tests. Fifteen samples (46.88%) were confirmed ND-positive, comprising 7 (43.75%) from Susut and 8 (50%) from Kintamani. Digestive disorders were the predominant clinical sign (86.66%), and characteristic lesions were most consistently observed in the lungs (100%), intestines, and cecal tonsils (93.33%). Chickens from Kintamani had a 1.29-fold higher risk of ND infection compared to Susut (OR 1.29; 95% CI: 0.3–5.4). The study demonstrates active ND circulation in Bangli Regency and reveals variability in clinical expression potentially influenced by management systems and biosecurity practices. By integrating field-based clinical assessment with pathological and HA/HI confirmation, this research strengthens practical diagnostic reliability in resourcelimited settings and provides region-specific epidemiological evidence to support targeted vaccination strategies, enhanced biosecurity implementation, and sustainable poultry health management in endemic areas.
Co-Authors Gunawan, I Wayan Nico Fajar Gusti Ayu Yuniati Kencana I Ketut Berata Ida Bagus Kade Suardana Ida Bagus Oka Winaya Inda Sabrina Latifah Ni Nyoman Werdi Susari Romy Muhammad Dary Mufa Tjokorda Sari Nindhia