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All Journal Animal Production : Indonesian Journal of Animal Production
W Suryapratama, W
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Utilization of Cow Milk Enriched with Conjugated Linoleic Acid to Decrease Body Weight, Cholesterol, Low Density Lipoprotein and to Increase Blood High Density Lipoprotein Suhartati, FM; Suryapratama, W; Rahayu, S
ANIMAL PRODUCTION Vol 14, No 2 (2012): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

An experiment to investigate the ability of cow milk enriched with conjugated linoleic acid to decrease body weight, total cholesterol, blood Low Density Lipoprotein (LDL), and to increase blood High Density Lipoprotein (HDL) has been conducted using in vivo experimental method. Research material consisted of 40 8-week-old white female rats (Rattus norvegicus) of Wistar strain (as an animal model). The method used was an experimental method with a Completely Randomized Design. The treatments tested were P1 = high-fat ration containing 27.66% fat (HF), P2 = HF + 5 ml of milk/head/day, P3 = HF + 10 ml of milk/head/day, P4 = low-fat ration containing 5% fat (LF). Each treatment was repeated five times to make 20 experiment units, each consisted of two rats. Body weight gain, total cholesterol, LDL-cholesterol and HDL-cholesterol were observed. The data obtained were then analyzed using analysis of variance followed by orthogonal contrast test. Orthogonal polynomials tests was applied to evaluate the response variables. The results showed that 10 ml/head/day of cow milk was needed to decrease body weight of hypercholesterolemic rats and 5 ml/head/day of cow milk was needed to decrease total cholesterol, LDL-cholesterol and to increase blood HDL-cholesterol of hypercholesterolemic rats.Keywords: cow milk, conjugated linoleic acid, body weight gain, cholesterol. Animal Production 14(2):70-76
The Physical Analyze of Local Grass Suhartati, FM; Suryapratama, W; Rahayu, S
ANIMAL PRODUCTION Vol 6, No 1 (2004): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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A research has been conducted to analyze physical characteristics of local grass. The aimed of this research was to observe grass physical characteristics, which are bulkiness, water regain capacity and water solubility. Also to observe correlation of water regain capacity to dry matter and organic matter digestibility as well as its degradation rate during 0, 12, 18 and 24 h incubation, in sacco, using 2 fistulae cows.  Five local grass were tested in this research, which are field grass, elephant grass, brachiaria grass, king grass and setaria. Fistulae cows consumed forages and concentrates with ratio of 70:30, minimum protein level of 12% and minimum TDN of 60%. Physical characteristics data that obtained then analyzed using analysis of variance. Furthermore, honestly significant different was also performed. Dry matter and organic matter digestibility data that obtained were analyzed with regression of physical characteristics.  Result showed that brachiaria grass has poor water regain capacity and water solubility.  There are positive linear correlation between water regains capacity with dry matter and organic matter digestibility. (Animal Production 6(1): 37-42 (2004) Key Words: Bulkiness, Water Regain Capacity, Water Solubility, Dry Matter and Organic Matter Digestibility
Effect of Supplementation of Branched Chain Fatty Acid on Colony of Ruminal Bacteria and Cell of Protozoa Suryapratama, W; Suhartati, FM
ANIMAL PRODUCTION Vol 11, No 2 (2009): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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A study was conducted to evaluate the potential of branched-chain volatile fatty acids (isobutyric, α-methylbutyric and β-methylbutiric) that supplemented into the diet on the colony of ruminal bacteria and the cell of protozoa population. Five progeny Friesian Holstein males with initial weight 348±29 kg were used in a 5x5 Latin square design (30-d periods). The basal diet composed of 55% forage and 45% concentrate containing 10.5 MJ ME/kg and 15% crude protein (CP).  There were five dietary treatments where A: basal diet, B: A+139 mg urea/kg W0.75, C: B+28 mg CaSO4/kg W0.75, D: C+0.05 mM isobutyric acid+0.05 mM β-methylbutyric acid, and E: D+0.05 mM α-methylbutyric acid.  Rearing period was 30 days, consists of feed adaptation period 20 days, then growth observation was done within the last 10 days. Collection of ruminal fluid was done within the last day of observation period, and took 3-4 h after the feeding.  The results showed that supplementation branched chain volatile fatty acids did not significant affect on the number of colonies of bacteria and protozoa population, but the significant effect (P<0.05) on the concentration of branched chain volatile fatty acids in the rumen fluid. The supplementation of α-methylbutyric (P <0.05) decreased of concentration of isobutyric and isovaleric in rumen fluid than the other treatments. It is concluded that supplementation of branched chain volatile fatty acids not used by rumen bacteria for their growth but for the elongation of fatty acid synthesis. The supplementation of branched chain volatile fatty acids was 0.05 mM not enough strong influence on the growth of colony of rumen bacteria. (Animal Production 11(2): 129-134 (2009) Key Words: rumen fermentation, branched-chain fatty acid, ruminal bacteria, protozoa
Ratio of Forage–Concentrate that Contain Fermented Tapioca by Product and Pollard and its Effect on Conjugated Linoleic Acid and Rumen Fermentation Parameters Suryapratama, W
ANIMAL PRODUCTION Vol 7, No 3 (2005): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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An experiment has been conducted to investigate the ratio of forage – concentrate that contain tapioca by product and pollard as fermented by Saccharomyces cerevisiae on conjugated linoleic acid and rumen fermentation parameters.  In vitro experiment with Completely Randomized Block Design was performed with six periods of collecting rumen fluid as block.  The rumen fluid was collected from fistulae cow in Animal Science Experimental Farm, University of Jenderal Soedirman. The diet consists of elephant grass as forages and concentrate that contain 45% of tapioca by product and pollard (ratio 1:1), urea added, then fermented with Saccharomyces cereviciae.  Another 55% of the concentrate contain of rice meal, coconut meal, soybean oil, mineral and salt.   Treatments were ratio of forage to concentrate i.e. 60:40; 70:30 and 80:20.  Therefore 18 experimental units exist.  Variables measured were VFA, N-NH3, conjugated linoleic acid concentration and protein synthesis of ruminal microorganism.  Analysis of variance with orthogonal polynomial resulted that the ratio of forage-concentrate has significant effect on VFA concentration  (P<0.05), N-NH3 and protein synthesis of ruminal microorganism (P<0.01). As forage increase, the VFA, N-NH3 and CLA linearly increase, but protein synthesis of ruminal microorganism linearly decrease.  The highest of CLA obtained in diet with forage- concentrate ratio 80:20. (Animal Production 7(3): 142-149 (2005) Key Words: VFA, N-NH3, Microbial Protein, Linoleic, CLA, Ratio Forage-Concentrate
Increasing Rumen Microbial Protein Synthesis with Additional Dietary Substrate of Saccharomyces cerevisiae and Soybean Oil Suryapratama, W; Suhartati, FM
ANIMAL PRODUCTION Vol 14, No 3 (2012): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Absract. A study with the purpose to increase microbial protein synthesis was carried out using in vitro experiment with 2x2 factorial. Completely Randomized Design were used in this study. The first factor was with or without substrate of Saccharomyces cerevisiae.   The second factor was the addition of 2 levels of soybean oil, 0% and 3%.  There were 4 treatments, each treatment was replicated 5 times so that there were 20 experimental units. The materials of this research were Napier grass, concentrates and substrate of Saccharomyces cerevisiae, consisted of a mixture of 30% rice bran, 20% tapioca by-product, 20% coconut meal, 20% corn, 9% pollard, 1% mineral, and soybean oil.  The rumen fluid as a source of rumen inoculum was taken immediately after a cattle was slaughtered in the slaughterhouse.  The variables measured were N-NH3 concentration and the product of rumen microbial protein synthesis.  Data were analyzed using analysis of variance, followed by a test of Honestly Significant Differences (HSD).  The conclusion was that the use of substrate of Saccharomyces cerevisiae increased rumen microbial protein synthesis as much as 9.42% and the use of 3% of soybean oil increased rumen microbial protein synthesis as much as 18.64%. Keywords: rumen microbe, Saccharomyces cerevisiae, soybean oil Abstrak. Sebuah studi dengan tujuan untuk meningkatkan sintesis protein mikroba dilakukan dengan menggunakan percobaan in vitro dengan pola faktorial 2x2. Rancangan Acak Lengkap digunakan dalam penelitian. Faktor pertama adalah dengan atau tanpa substrat Saccharomyces cerevisiae. Faktor kedua adalah penambahan 2 level minyak kedelai, 0 % dan 3 %. Penelitian melibatkan 4 perlakuan dan setiap perlakuan diulang 5 kali sehingga ada 20 unit percobaan. Materi penelitian ini adalah rumput gajah, konsentrat dan substrat Saccharomyces cerevisiae, terdiri dari campuran 30 % dedak padi, 20 % produk samping tapioka, 20 % bungkil kelapa, 20% jagung, 9% pollard, 1% mineral, dan minyak kedelai. Cairan rumen sebagai sumber inokulum rumen diambil sesaat setelah ternak disembelih di rumah potong. Variabel yang diukur adalah konsentrasi N-NH3 dan produk dari sintesis protein mikroba. Data dianalisis dengan menggunakan analisis variansi, dilanjutkan dengan uji Beda Nyata Jujur (HSD). Kesimpulannya adalah bahwa penggunaan substrat Saccharomyces cerevisiae meningkatkan sintesis protein mikroba sebesar 9,42% dan penggunaan 3% minyak kedelai meningkatkan sintesis protein mikroba rumen sebesar 18,64%. Kata kunci : mikroba rumen, Saccharomyces cerevisiae, minyak kedelai W Suryapratama and FM Suhartati/Animal Production 14(3):155-159, September 2012
Co-Authors FM Suhartati, FM S Rahayu