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Utilization of Cow Milk Enriched with Conjugated Linoleic Acid to Decrease Body Weight, Cholesterol, Low Density Lipoprotein and to Increase Blood High Density Lipoprotein Suhartati, FM; Suryapratama, W; Rahayu, S
ANIMAL PRODUCTION Vol 14, No 2 (2012): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

An experiment to investigate the ability of cow milk enriched with conjugated linoleic acid to decrease body weight, total cholesterol, blood Low Density Lipoprotein (LDL), and to increase blood High Density Lipoprotein (HDL) has been conducted using in vivo experimental method. Research material consisted of 40 8-week-old white female rats (Rattus norvegicus) of Wistar strain (as an animal model). The method used was an experimental method with a Completely Randomized Design. The treatments tested were P1 = high-fat ration containing 27.66% fat (HF), P2 = HF + 5 ml of milk/head/day, P3 = HF + 10 ml of milk/head/day, P4 = low-fat ration containing 5% fat (LF). Each treatment was repeated five times to make 20 experiment units, each consisted of two rats. Body weight gain, total cholesterol, LDL-cholesterol and HDL-cholesterol were observed. The data obtained were then analyzed using analysis of variance followed by orthogonal contrast test. Orthogonal polynomials tests was applied to evaluate the response variables. The results showed that 10 ml/head/day of cow milk was needed to decrease body weight of hypercholesterolemic rats and 5 ml/head/day of cow milk was needed to decrease total cholesterol, LDL-cholesterol and to increase blood HDL-cholesterol of hypercholesterolemic rats.Keywords: cow milk, conjugated linoleic acid, body weight gain, cholesterol. Animal Production 14(2):70-76
The Physical Analyze of Local Grass Suhartati, FM; Suryapratama, W; Rahayu, S
ANIMAL PRODUCTION Vol 6, No 1 (2004): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

A research has been conducted to analyze physical characteristics of local grass. The aimed of this research was to observe grass physical characteristics, which are bulkiness, water regain capacity and water solubility. Also to observe correlation of water regain capacity to dry matter and organic matter digestibility as well as its degradation rate during 0, 12, 18 and 24 h incubation, in sacco, using 2 fistulae cows.  Five local grass were tested in this research, which are field grass, elephant grass, brachiaria grass, king grass and setaria. Fistulae cows consumed forages and concentrates with ratio of 70:30, minimum protein level of 12% and minimum TDN of 60%. Physical characteristics data that obtained then analyzed using analysis of variance. Furthermore, honestly significant different was also performed. Dry matter and organic matter digestibility data that obtained were analyzed with regression of physical characteristics.  Result showed that brachiaria grass has poor water regain capacity and water solubility.  There are positive linear correlation between water regains capacity with dry matter and organic matter digestibility. (Animal Production 6(1): 37-42 (2004) Key Words: Bulkiness, Water Regain Capacity, Water Solubility, Dry Matter and Organic Matter Digestibility
Preliminary Study on Keratinase from Two Indonesian Isolates Rahayu, S; Syah, D; Suhartono, MT
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Keratinases (E.C.3.4.99.11) constitute a group of enzymes capable of disrupting the highly stable keratin structure consisting of disulphide, hydrogen, and hydrophobic bonds in the form of α-helices and β-sheets B. licheniformis MB-2 and Bacillus sp. MTS are two feather-degrading bacteria isolated from Tompaso crater at North Sulawesi and sulfuric land around Tangkuban Perahu in West Java. They were both capable of breaking down whole chicken feathers. In addition both isolates were capable of degrading other proteinous substrates rich in beta structure such as coccon, silk, human hair and fish scales. Result of fermentation experiment implied that addition of nitrogen sources (0.02% yeast extract and 0.02% tryptone) to the basal medium increased keratinase production. Our experiments showed that keratinase production of Bacillus sp. MTS was higher and faster than that from B. licheniformis MB-2. Maximum extracellular keratinase activity of the enzyme derived from B. licheniformis was obtained during stationary phase at 72 h, while Bacillus sp. MTS  was reached at 48 h. Disulfide reductase activity also detected in the extracellular fluid of Bacillus sp. MTS.  The maximum condition for extracellular keratinase activity was 55oC and the enzyme showed  two maximum pHs :  pH 8.0 and pH 10. The zymogram analysis indicated sixth protein bands  of  17, 25, 32, 53, 96 and 122 kD which were able to hydrolyze gelatin substrate in-situ. (Animal Production 12(1): 60-68 (2010)Key Words : Bacillus, feather, keratinase, disulfide reductase
Analisis Critical Path Method dan Time Cost Trade Off dalam Optimasi Waktu dan Biaya Pengerjaan Proyek Pembangunan Rumah Sakit Rahayu, S; Nurwan; Wungguli, D
JURNAL ILMIAH MATEMATIKA DAN TERAPAN Vol. 19 No. 2 (2022)
Publisher : Program Studi Matematika, Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/2540766X.2022.v19.i2.16176

Abstract

Proyek merupakan suatu usaha/aktivitas yang kompleks, tidak rutin, dibatasi oleh waktu, anggaran, resources, dan spesifikasi performansi yang dirancang untuk memenuhi kebutuhan konsumen. Suatu proyek yang besar tentu saja memerlukan waktu dan biaya yang tepat untuk memperoleh durasi pembangunan serta biaya yang dikeluarkan lebih optimal. Optimalisasi merupakan proses pengajuan durasi proyek untuk mendapatkan solusi yang efisien dengan menggunakan berbagai alternatif yang dapat ditinjau dari segi biaya serta waktu. Dalam penelitian ini bertujuan untuk menentukan kegiatan mana yang termasuk kategori jalur kritis, melakukan perbandingan upah tenaga kerja antara penggunaan jam kerja (lembur) dengan penggunaan 2 shift kerja menggunakan metode Time Cost Trade Off, dan menentukan waktu yang paling optimal untuk menyelesaikan proyek tersebut. Tahapan penelitian ini adalah mencari jalur kritis dengan menggunakan Critical Path Method, melakukan analisis durasi dan biaya upah tenaga kerja pada jalur kritis dengan variabel penambahan jam kerja (lembur) dan penggunaan 2 shift kerja menggunakan pendekatan Time Cost Trade Off. Berdasarkan hasil analisis dan perhitungan, biaya upah tenaga kerja normal dengan durasi proyek 210 hari ialah sebesar Rp. 1.542.804.496,00. Kemudian percepatan dilakukan sehingga menghasilkan waktu optimal untuk penyelesaian proyek tersebut yaitu penggunaan 2 shift kerja, dengan durasi proyek menjadi 151 hari dengan biaya sebesar Rp. 1.488.970.000 dengan menghemat waktu 59 hari dengan selisih biaya sebesar Rp. 53.834.496,00 dari biaya normal.