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All Journal Animal Production : Indonesian Journal of Animal Production
M Soejono, M
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Effect of Administration of Lignocellulose-Degrading Fungi Isolated from Herbivore’s Gastrointestinal Tract for Fiber Degradation Wahyudi, A; Cahyanto, MN; Soejono, M; Bachruddin, Z
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Isolation and selection of lignocellulose-degrading fungi from compartment of herbivore’s gastrointestinal tract were predicted found fungi that have superiority to degrade lignin, xylan, and cellulose. Lignocellulose-degrading fungi were isolated from compartment of buffalo’s and horse’s gastrointestinal tract and  also elephant dung with malt extract agar, using cellulose, xylan, and tannic acid as selective substrate. Morphological and biochemical test had been done to get superior isolates. This study showed that lignocellulose-degrading fungi could be found in all sample of buffalo’s and horse’s gastrointestinal tract and also elephant dung. The highest number of lignin, xylan and cellulose-degrading isolates respectively were found from buffalo’s cecum (5 isolates), buffalo’s colon (19 isolates), and buffalo’s colon (326 isolates). The highest isolates activity of lignolytic, xylanolytic, and cellulolytic respectively were reached from horse’s cecum (2.38), horses cecum (6.67), and buffalo’s colon (5.60). Meanwhile the highest enzymes activities were reached from horse’s cecum (0.166 Unit/g protein), horse’s cecum (5.037 Unit/g protein) and buffalo’s colon (2.488 Unit/g protein). From this study could be concluded that lignocellulose-degrading fungi could be found from all compartment of herbivore’s gastrointestinal tract. Based on quantitative and qualitative selection, lignolytic from horse’s cecum, xylanolytic from horse’s cecum and cellulolytic from buffalo’s colon were superior isolates and predicted as Aspergillus sp genus. Administratiton of Aspergilillus sp inoculum into rumen fluid medium elevated significantly dry matter, organic matter,  crude fiber, neutral detergent fiber, and acid detergent fiber digestibility as 4.55%; 4.45%; 6.69%; 11.65% and 8.23% respectively. (Animal Production 12(1): 21-28 (2010)Key Words : lignocellulose-degrading fungi, herbivore’s gastrointestinal tract, fiber degradation.
Effect of Protected Kapok Seed Oil Supplementation on In Vitro Ruminal Lipid Status Widiyastuti, Widiyanto; Soejono, M; Hartadi, H; Bachrudin, Z
ANIMAL PRODUCTION Vol 11, No 2 (2009): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

This investigation was conducted to study the influence of protected kapok seed oil  (KSO) supplementation on ruminal lipid status. Kapok seed oil (KSO), field grass (FG), Javanese Thin Tailed Sheep (JTTS) sheep rumen fluid, were used as experimental material. There were two treatment factors, namely : KSO supplementation as factor I, and KSO protection (throughout formation of Ca- salt after lipolysis by KOH) as factor II. Factor I consist of 4 supplementation levels, namely : 0% (S0); 5% (S1); 10% (S2), and 15% (S3), respectively, wheareas factor II consist of 5 protection levels, namely : 0% (P0); 25% (P1); 50% (P2); 75% (P3), and 100% (P4), repectivelly. Amount of 15 treatment combination were formed with control, namely without KSO supplementation and without protection (S0P0). The measured variables included : relative proportion of volatile fatty acids (VFAs), i.e. : acetic acid, propionic acid, and butyric acid. In addition, the iodine number (IN) and relative proportion of long chain fatty acid (LCFA), i.e. : stearic acid and linoleic acid )omega 6 polyunsaturated fatty acid) were also measured. Collected data were analyzed statistically by analysis of variance with factorial treatment pattern in completely randomized design. The result of this investigation showed that supplementation 10% or upper KSO without protection decreased asetis acid/propionic (A/P) ratio. Ratio of A/P tend increased again with protected KSO supplementation, but on 10 and 15% supplementation levels still lower than control (1.47–2.99 vs 3.36). Protected KSO supplementation increased the relative proportion of linoleic acid and unsaturation rate of ruminal fatty acid (P<0,05) with the higher iodine number, a long with enhancing of supplementation level. Relative proportion of linoleic acid and iodine  number (IN) in S1P0, S2P0, S2P3, S2P4 and S3P4 treatment groups were : 17,65 and 10,32; 33,07% and 18,67; 42,29% and 41,95; 45,01% and 47,40; 45,17and 50,99%, respectively. (Animal Production 11(2): 122-128 (2009) Key Words: supplementation, kapok seed oil, ruminal lipid status
Co-Authors A Wahyudi H Hartadi, H MN Cahyanto, MN Widiyanto Widiyastuti, Widiyanto Z Bachruddin, Z Z Bachrudin, Z