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All Journal Animal Production : Indonesian Journal of Animal Production
EA Rimbawanto, EA
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem Rahayu, Sri; Suhartati, FM; Rimbawanto, EA; Iriyanti, Ning
ANIMAL PRODUCTION Vol 5, No 2 (2003): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Rumen is an interesting ecosystem for microbial exploration and their products. Isolation of the chitinolytic bacteria from the rumen ecosystem found 109 colonies that produced clear zone, 84 colonies (86%) anaerobic and 17 colonies (14%) aerobic. Clear zone appeared in the third and fourth days incubation. Four potential isolates were chosen for identification purposes. Results showed that the bacteria were sticky, gram-positive, motile, endospore-forming, mesophilic and aerobic. It was supposed to Bacillus spp. the optimal pH and temperature to produce chitinase from isolate 18 are pH 6.0 and temperature of 35-40ºC. Divalent cations Mg, Ca, Zn, and Mn increase chitinase activity, while Cu and Co inhibit enzyme activity. When isolate 18 was grown on shrimp waste meal, it showed aptimal activity on the fifth days incubation. (Animal Production 5(2): 73-78 (2003) Key Words : Isolation, Identification, Chitinolytic Bacteria, Rumen
Increasing of Energy Quality of Waste Product with Trichoderma and Aspergillus Suwandyastuti, SNO; Subarjo, B; Rimbawanto, EA; Prayitno, Prayitno; Irawan, I; Zubaidah, S
ANIMAL PRODUCTION Vol 1, No 1 (1999): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Biomasa of agricultural residues are potensial as ruminant feeds. However due it is low palatability, digestibility and nutritive value limited their use. In order to improve their use, treatment need to be applied. Biological treatment by using microba seems to be an alternative because of their capability with no pollution problems. The first experiment aims to select the microorganism which have a potensial to degrade the crude fiber, based the production of reduction sugar. The second experiment aims to improve the protein and amino acid on rice straw, cassava, waste, and rice husk, by inoculated the starter of Candida utilis and or Sacharomyces cerevise. The second experiment has been conducted on Animal Nutrition and Feed Laboratory, Faculty of Animal Husbandry UNSOED for eight month Fermentation trial has been done in semi solid media, by the method of Kjic (1964), in Batch System, Variables measure were: (1) reduction sugar, (2) cellulose, (3) protein, (4) amino acids, (5) cellulase activity, (6) essensial mineral and (7) energy. Based on the all variables measured that were conclused that the quality of  rice straw can be improved by mixed culture of  T, viride – S. cerevise, the rice husk by A. niger – C. utilis, T. viride – C. utilis and A. niger – S cerevise while for cassava waste by  A. niger – S. cerevise and A. niger – C. utilis  (Animal Production 1(1) : 10-16 (1999). Key Words: Waste Product, Energy, Microorganism
The Effect of Soybean Hulls Substitution Levels to Corn in The Ration on Energy and Protein Digestibility and on Sheep Performance Rimbawanto, EA; Iriyanti, N
ANIMAL PRODUCTION Vol 2, No 2 (2000): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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An experiment was conducted to investigate the effect of soybean hulls levels substitution in to corn as ration in twenty seven rams. The animal had a mean of live weight of 12.86 ± 1.94 kg. Levels of substitution were 0, 50, and 100% or 0, 25, and 50% ration dry matter basis and rations were iso-nitrogeneous. Analysis of variance were conducted for completely randomized design and polynomial orthogonal test were applied for the statistical analysis. Inclusion of soybean hulls in 50% ration dry matter had no effect on daily gain (99.71 ± 22.97 g), and digestible crude protein (62.43 ± 6.56 %). However, dry matter consumption tended to increase linearly and as soybean hulls level increase in the ration (P<0.01). The digestible energy significantly decrease linearly (P<0.01), whereas intake of the energy had same was 1.27 ± 0.33, 1.44 ± 0.17, and 1.23 ± 0.19 Mcal/day for ration containing 0, 25, 50% of soybean hulls, respectively. It was concluded that the soybean hulls may be substituted for corn. (Animal Production 2(2): 98-103 (2000) Key words : soybean hulls, corn, digestibility, daily gain.
The Utilization of Soybean Hulls as Energy Resources on Ration Local Sheep Rimbawanto, EA; Iriyanti, Ning
ANIMAL PRODUCTION Vol 3, No 1 (2001): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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An experiment was conducted to investigate the effects of soybean hulls as a ration in twenty seven rams. The animal had a mean of live weight of 12.863 ± 1.934 kg. Levels of soybean hulls were 0, 50, and 100 %  or 0,25, and 50 % in ration dry matter basis and rations were  iso-nitrogenous. The experiment were use Completely Randomized Design, data collected was analyzed using analysis of variance and polynomial orthogonal test.  Inclusion of soybean hulls in 50% ration dry matter had no effect on daily live weight gain (90.65±20.88 g), nitrogen, calcium and phosphor balances positive. However, dry matter consumption tended to increase linearly and as soybean hulls level increase in the ration (P<0.01). The digestible energy and NDF significantly decrease linearly (P<0.01), whereas intake of the energy was similar i.e. 122±0.39, 1.44±0.17, and 1.23±0.19 Mcal/day but NDF tended to increase for ration containing 0, 25, 50% of soybean hulls, respectively. The digestible crude protein is significantly quadratic (P<0.01), due to the release of energy and N are synchronized in 25% of soybean hulls in ration dry matter. It was concluded that soybean hulls can be used as a sources of energy and substitute for corn. (Animal Production 3(1): 5-11 (2001) Key Words: Local ram, soybean hulls, live weight gain, digestibility
Co-Authors B Subarjo, B FM Suhartati, FM I Irawan, I N Iriyanti, N Ning Iriyanti Prayitno Prayitno S Zubaidah, S S.N.O. Suwandyastuti SRI RAHAYU