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Quality Of Pe Buck Sperm Stored In Different Packaging Pangestu, M; Soenarjo, CH; Siswadi, Rachmawati W; Sugiyatno, Sugiyatno; Tagama, Taswin R; Saleh, Dadang M; Haryati, Haryati; Sutarmo, Sutarmo; Setyawati, SJA
ANIMAL PRODUCTION Vol 1, No 1 (1999): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

AI for cattle has been develop in Indonesia in contrast, AI for small ruminants (sheep and goat) are less developed. Its due to the lack of facilities, processing and packaging during storage. This research aims to compare the effectivity and efficiency of two different packaging technique (test tube and straw). Test tube and 0.25 ml mini straw were tested . Semen was obtained from adult PE buck (3 yr) after collection by using  artificial vagina. The fresh semen was then evaluated and diluted 5 folds with 2.9 percent Na-citrate. Diluted semen then packed in test tube and mini straw, and stored in refrigerator (100C) for 7 days. Observations were done everyday on sperm motility, abnormality and percentage of  live sperm. Observation were made at 370C. Observation on fresh ejaculate showed that semen has 6x 109/ml concentration, 90 percent motility, 8 percent abnormality and  95 percent  live sperm. Five folds dilution reduced sperm concentration to 1.2 x 109/ml, but did not change sperm motility, abnormality and percent of live sperm. Sperm was then packed according  to the treatments. Storage in both packaging did significantly reduce  (P<0.01) sperm motility and percent live sperm, but not for sperm abnormality. All sperm stored in test tube were classified death at day 5 storage. However, sperm in the straw were remain live eventhough the percentages was low. It can be concluded that straw has a better result than test tube. Eventhough sperm motility was extremely low, it remains valuable for cervical insemination. (Animal Production 1(1) : 24-29 (1999).  Key Words : Sperm, PE Buck, Straw, Test Tube
Immunity Response of Gumboro and ND Vaccination Result which Given with Time Spacing in Broiler Chicken Yuwono, Endro; Setyawati, SJA
ANIMAL PRODUCTION Vol 3, No 2 (2001): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

An experiment was planed to know  the possibility of negative effect on Gumboro vaccination program. This program has a possibility to cause “Sick” condition on fabrious bursal of broiler chicken. From that case, it need time spacing for subsequent vaccination program, for instance ND vaccination program.  Time spacing is very importance to broiler chicken for recovering that “Sick” condition because of Gumboro vaccination effect. The purpose of his research was to know the best time spacing between Gumboro and ND vaccination program on broiler chicken. An experimental  method was used in this research, and the material used was 216 broiler chickens. A 3x4 factorial arrangement (Gumboro vaccination as factor A and ND vaccination  as factor B) with three broiler chickens per unit and six replicates of each treatment combination was used in the  research. Variables tested of  this experiment HI ND antibody titer, body weight  of 4 weeks old, and the symptom appeared after vaccination program. Anava was used to analyze the data obtained and used orthogonal polynomial for subsequent analysis. The research  results showed that the best time spacing was eight days and there was no symptom  appeared after  Gumboro  vaccination program. There were no significantly influence of vaccination treatments on broiler chicken health and body weight. (Animal Production 3(2): 67-73 (2001) Key Words: Vaccination, symptom, antibody
Oocyst Simultaneous Infection to Increase Broiler Immunity from Coccidiosis Setyawati, SJA; Yuwono, E
ANIMAL PRODUCTION Vol 8, No 1 (2006): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Coccidiosis is one of parasitic disease caused by Eimeria which is frequently occurs in broiler chick. The disease produces enteritis diarrhea that affects viability and growth.  Stimulating its immune could be done by repeated light oocyst infection.  This research purposed to study infection level and frequency of Eimeria tenella on coccidiosis immunity and broiler performance.   Factorial 2 X 3 was applied with infection level as the first factor: 10.000 oocyst (B1); and 20.000 oocysts (B2). The second factor was infection frequency: once (A1); twice (A2); and three times (A3). The first infection was at 10 days old and the next was 7 days later. Each experiment unit had 8 broilers, and it was replicated 4 times.  On 30 days old, challenge test was conducted by infection using 25.000 oocysts per broiler.  Parameter observed were oocyst elimination, caecum treatment score and clinical symptom post inoculation and post test, weekly body weight, final weight, and feed conversion.  Data were analyzed using variance analysis and Honestly Significant Difference (HSD) test. Weekly body weight and clinical symptom were described.  The result indicated that broiler infected by Eimeria tenella oocyst simultaneously with 20.000 oocysts caused immunization. The number of oocyst did not have significant effect on the performance. (Animal Production 8(1): 72-77 (2006) Key Words : Eimeria tenella, enteritis diareal, performance, immunization.