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All Journal Animal Production : Indonesian Journal of Animal Production
Muh Affan Mu’in, Muh Affan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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A 24-bp Indel (Insertion-Deletion) Polymorphism in Promoter Prolactin Gene Lumatauw, Sintje; Mu’in, Muh Affan
ANIMAL PRODUCTION Vol 18, No 1 (2016): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.837 KB) | DOI: 10.20884/1.anprod.2016.18.1.526

Abstract

Prolactin in chicken (cPRL) is a polypeptide hormone that plays a crucial role in incubation and brooding behavior. This hormone is coded by a gene that located at chromosome 2. The objective of this study was to investigate 24-bp indel polymorphism in promoter prolactin gene of Papua local chickens. A number of 60 DNA samples came from 60 Papua local chickens (25 males and 35 females) were used in this study.  PCR (Polymerase Chain Reaction) method with a pair of specific primer was applied and a specific DNA fragment with 130 and/or 154 bp contained 24-bp indel was successfully amplified. Results of electrophoresis run on 1.5 % agarose gel showed three genotypes: II, ID and DD with the frequency of 6,7%, 48.3% and 45% respectively. The frequency of I allele was 0.31 and D allele was 0.69. Results indicated  that the I allele frequency was medium in Papua local chickens population. Results therefore suggest that incubation behavior in hen population of Papua local chickens is easy to eliminate by increasing the I allele frequency in the population.
Co-Authors Sintje Lumatauw, Sintje