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All Journal Animal Production : Indonesian Journal of Animal Production Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences)
Sintje Lumatauw, Sintje
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Veterinary

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 1 
A 24-bp Indel (Insertion-Deletion) Polymorphism in Promoter Prolactin Gene Lumatauw, Sintje; Mu’in, Muh Affan
ANIMAL PRODUCTION Vol 18, No 1 (2016): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.837 KB) | DOI: 10.20884/1.anprod.2016.18.1.526

Abstract

Prolactin in chicken (cPRL) is a polypeptide hormone that plays a crucial role in incubation and brooding behavior. This hormone is coded by a gene that located at chromosome 2. The objective of this study was to investigate 24-bp indel polymorphism in promoter prolactin gene of Papua local chickens. A number of 60 DNA samples came from 60 Papua local chickens (25 males and 35 females) were used in this study.  PCR (Polymerase Chain Reaction) method with a pair of specific primer was applied and a specific DNA fragment with 130 and/or 154 bp contained 24-bp indel was successfully amplified. Results of electrophoresis run on 1.5 % agarose gel showed three genotypes: II, ID and DD with the frequency of 6,7%, 48.3% and 45% respectively. The frequency of I allele was 0.31 and D allele was 0.69. Results indicated  that the I allele frequency was medium in Papua local chickens population. Results therefore suggest that incubation behavior in hen population of Papua local chickens is easy to eliminate by increasing the I allele frequency in the population.
PERFORMANS BOBOT BADAN AYAM LINGNAN UMUR 8 – 16 MINGGU YANG DIPELIHARA DI MANOKWARI PAPUA BARAT Lumatauw, Sintje; Iroth, Wandy Joudy; Chotimah, Siti Chusnul
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 5 No 1 (2010): JURNAL ILMU PETERNAKAN
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.011 KB)

Abstract

A study was initiated to identify body weight performance of Chinese traditional Lingnan chicken at 8 to 16 weeks of age. Both male and female body weight and feed consumption were recorded. Four hundred and sixty eight Lingnan chickens (108 males and 360 females) were randomly distributed to 12 experimental cage unit of 3 x 4 m followed a Completely Randomized Design. The chickens were treated with four different ration combinations which consisted of commercial ration, yellow corn, and rice brand. Each treatment was replicated three times. Result of the study showed that the male Lingnan chicken was heavier than the females at each observation. The different body weight was 335, 498, 557, 637 and 553 grams at the age of 8, 10, 12, 14 and 16 weeks, respectively. At 16 weeks, the male Lingnan average body weight was 2927 grams while the female was 2374 grams. Four different ration combinations were not significantly affected the chicken body weight (P>0.05).
UJI KUALITAS TELUR AYAM RAS DI KOTA MANOKWARI Kasmiati, .; Lumatauw, Sintje; Sumpe, Iriani
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 8 No 1 (2018): JURNAL ILMU PETERNAKAN
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.287 KB) | DOI: 10.30862/jipvet.v8i1.28

Abstract

A study was conducted to evaluate table eggs in Manokwari city. A total of 300 eggs were examined for external and internal qualities. The eggs were gathered from different places: local farmer for local eggs (1 respondent), markets for imported eggs (2 respondents)), supermarkets (4 respondents), egg distributors (1 respondent), and stalls (10 respondents)). The egg examinations were done twice, first was the time when no ship for egg transport came to Manokwari (period 1) to assume that the eggs had been kept for quite long before reached the consumers and second was the time when ship for egg transport that just arrived to Manokwari with the assumption that he eggs were still relatively fresh (periode 2). Results showed that the majority of table eggs in Manokwari had brown shells followed by spotted and light brown, all with oval shapes. Eggs gathered at period 2 were larger than those of period 1; local eggs were significantly heavier than imported eggs due to the difference of egg freshness. The local eggs of period 2 showed a very good air sac with AA quality, while the imported eggs had the air sac quality for A and B. The highest yolk score (8.88) were observed at local eggs at period 2.
Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung Inggriani, Kiki; Tethool, Angelina Novita; Lumatauw, Sintje
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 10 No 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.709 KB) | DOI: 10.46549/jipvet.v10i1.67

Abstract

Antioxidants can inhibit the oxidation process by reacting with free radicals, which in turn, maintain the quality of spermatozoa. This study aims to determine the viability and the abnormality of “ayam kampung” (Gallus gallus) spermatozoa after being diluted in ringer lactate infused with “sarang semut” (Myrmecodia Sp) extract. RAL is the research method used, utilizing 4 different Gallus gallus as test subjects. The variables observed are the abnormality and the viability of the spermatozoa sample treated with different amounts of Myrmecodia Sp extract, P0: 0 ml, P1: 0.02 ml, P2: 0.03, and P3: 0.04 ml. The abnormality rate for each sample is as follows; P0: 14 ± 4.05%, P1: 13.5 ± 3.78%, P2: 3.5 ± 1.23% and P3: 12.83 ± 5.04 %. Using the variance analysis, the result showed that the addition of Myrmecodia Sp extract in ringer lactate solution had little to no effect on causing spermatozoa abnormalities. Furthermore, the viability of spermatozoa for each sample is; P0: 14 ± 4.05%, P1: 13 ± 3.78%, P2: 13 ± 1.22% and P3: 12 ± 5.04%. Based on the variance analysis, the result showed that Myrmecodia Sp extract had a significant effect (P <0.05) on the spermatozoa viability. The addition of Myrmecodia sp extract to ringer lactate diluter had little to no effect on the abnormality of the Gallus gallus’ spermatozoa but had a significant effect on the spermatozoa’s viability.
Heritabilitas Produki Telur Ayam Lokal Papua Berbeda Genotip dari Lokus 24- bp Insertion-Deletion dalam Promotor Gen Prolaktin Mu'in, Muhammad Affan; Lumatauw, Sintje
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 11 No 2 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i2.161

Abstract

The heritability (h2) of a trait shows phenotypes variance of the trait caused by additive genetic variance. The h2 value is used to estimate the quantitative trait breeding value of livestock in order to improve these traits through selection. This study aims to estimate the h2 of egg production characteristics in Papua local chickens with different genotypes from the 24-bp InDel (Insertion-Deletion) locus in the prolactin gene promoter region (24-bp InDel/cPRLp locus). A total of 13 pairs of Papua local chickens consisting of 3 pairs of II genotypes (♂II x ♀II) and 11 female offspring, 5 pairs of ID genotypes (♂ID x ♂ID) and 19 female offspring, and 5 pairs of DD genotypes (DD x ♀DD) and 17 female offspring were used in this study. Observations were made on the characteristics of egg production in female offspring of each genotype group. The variance component for h2 estimation is obtained by the one-way analysis of variance method and the separation of the variance components for single pairs. The results showed that the h2 at first laying of eggs in all genotype groups was moderate (0.10 to 0.30); the h2 of the number of eggs produced from the time they first laid eggs until the age of 240 days in II and ID genotype groups was high (> 0.3), while in the DD genotype group was classified as moderate (0.10 to 0.30); and the h2 of egg weight in all genotype groups was moderate (0.10 to 0.30). The high h2 of a trait indicates that the trait is more dominated by additive genes and is more responsive to the selection treatment.
Response of Egg Number to Selection of Different Genotypes of 24-bp Insertion-Deletion Locus in the Promoter of Prolactin Gene of Papua Local Chickens Mu'in, Muhammad Affan; Lumatauw, Sintje
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.75

Abstract

This study aims to estimate the response of the number of eggs produced up to 240 days of age (EN240) to the selection of Papua local chickens (PLC) with different genotypes from the 24-bp Indel cPRLp locus. A total of 68 PLCs were taken randomly from several breeders in Manokwari as the basic population for selection (G0). Genotype identification of the 24-bp Indel cPRLp locus was performed G0. Based on the distribution of these genotypes, mating pairs were randomly formed G0-II, G0-DD and G0-ID to produce G1-II, G1-DD, and G1-Control (G0-ID offspring). Heritability of EN240 in G1-II and G1-DD populations were estimated in full-sib (single pairs mating). Individual selection based on breeding value EN240 was carried out on G1-II (♀) and G1-DD (♀) to form the selection generation (GS): GS-II (♀) and GS-DD (♀). Selection was also carried out on G1-II (♂) and G1-DD (♂) based on body weight at 240 days (BW240) to become selected GS-II (♀) and GS-DD (♀) mating partners. GS-Control was also formed through 25% random sampling from G1-Control (♂ and ♀). GS mating resulted in the second generation (G2): G2-II, G2-DD, and G2-Control. Age at first laying (AFL), EN240, and mean egg weight (EWA) in hens of G1, GS, and G2 were recorded. Response to selection for EN240 was calculated by two methods predicted selection response (Rp) and actual selection response (Rr). Both methods of calculation yield positive and high values. In actual response (Rr), PLC in II genotypes group are more responsive to the selection treatment than DD genotype group. Selection increase EN240 impact accelerate of AFL and lower the EWA, because of their negative genetic correlation.
Co-Authors . Kasmiati Alnita Baaka Chotimah, Siti Chusnul Inggriani, Kiki Iriani Sumpe Iroth, Wandy Joudy Mu'in, Muhammad Affan Muh Affan Mu’in, Muh Affan Murwanto, A. Gatot Tethool, Angelina Novita