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All Journal Animal Production : Indonesian Journal of Animal Production
MT Suhartono, MT
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Preliminary Study on Keratinase from Two Indonesian Isolates Rahayu, S; Syah, D; Suhartono, MT
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Keratinases (E.C.3.4.99.11) constitute a group of enzymes capable of disrupting the highly stable keratin structure consisting of disulphide, hydrogen, and hydrophobic bonds in the form of α-helices and β-sheets B. licheniformis MB-2 and Bacillus sp. MTS are two feather-degrading bacteria isolated from Tompaso crater at North Sulawesi and sulfuric land around Tangkuban Perahu in West Java. They were both capable of breaking down whole chicken feathers. In addition both isolates were capable of degrading other proteinous substrates rich in beta structure such as coccon, silk, human hair and fish scales. Result of fermentation experiment implied that addition of nitrogen sources (0.02% yeast extract and 0.02% tryptone) to the basal medium increased keratinase production. Our experiments showed that keratinase production of Bacillus sp. MTS was higher and faster than that from B. licheniformis MB-2. Maximum extracellular keratinase activity of the enzyme derived from B. licheniformis was obtained during stationary phase at 72 h, while Bacillus sp. MTS  was reached at 48 h. Disulfide reductase activity also detected in the extracellular fluid of Bacillus sp. MTS.  The maximum condition for extracellular keratinase activity was 55oC and the enzyme showed  two maximum pHs :  pH 8.0 and pH 10. The zymogram analysis indicated sixth protein bands  of  17, 25, 32, 53, 96 and 122 kD which were able to hydrolyze gelatin substrate in-situ. (Animal Production 12(1): 60-68 (2010)Key Words : Bacillus, feather, keratinase, disulfide reductase
Age Maturity of Female Japanese Quails Fed Diets Containing Katuk Leave Meal (Sauropus androgynus L.Merr. Wiradimadja, R; Piliang, WG; Suhartono, MT; Manalu, W
ANIMAL PRODUCTION Vol 9, No 2 (2007): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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This experiment was conducted to study the effects of katuk leave meal on sexual maturity in Japanese quail.  Three hundred female Japanese quails, with 4 weeks age, were assigned into a completely randomized design with 4 treatments i.e., R1 (diet with neither katuk leave meal nor cholesterin), R2 (diet without   katuk leave meal + 0.10% cholesterin), R3 (diet with 15% katuk leave meal without cholesterin), and R4 (diet with 15% katuk leave meal + 0.10% cholesterin). The parameters measured were estradiol hormone in plasma, and age maturity (five percent henday production). Estradiol hormone was measured with Radioimmunoassay. The data were collected and analyzed by using completely randomized design. The results showed that the addition of 15% katuk leave meal in the diet significantly decreased the estrogen concentration (P<0.05), so that slow down on the age maturity of female Japanese quail. (Animal Production 9(2): 67-72 (2007)  Key Words : Katuk leave meal, estradiol hormone, age maturity, quail
Co-Authors D Syah, D R Wiradimadja, R S Rahayu W Manalu WG Piliang, WG