KHAMDAN KHALIMI
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Jl. PB. Sudirman Denpasar Bali 80231

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Uji Antagonistik Bakteri Penghasil Indole Acetic Acid Terhadap Jamur Helminthosporium maydis Secara In Vitro HELENA TAMPUBOLON; KHAMDAN KHALIMI; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.4, Oktober 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Antagonistic Test of Indole Acetic Acid Producing Bacteria Against Helminthosporium maydis In Vitro Helminthosporium maydis is the cause of important diseases in maize, including leaf blight. Leaf blight is the most dominant disease because it causes large losses. The use of biological agents is one way to treat leaf blight that is environmentally friendly. One of these biological agents is IAA-producing bacteria. This research was conducted in vitro. The results showed that there were several IAA-producing bacteria that were able to inhibit the growth of H. maydis in vitro with the highest inhibition in the treatment of B. thuringiensis GR12 isolates of 96.44% when compared to controls. IAA-producing bacteria were able to inhibit the growth of H. maydis biomass with the percentage of inhibition ranging from 73.97% - 80.82%. The treatment of IAA-producing bacterial filtrate at a concentration of 20%-90% was able to inhibit the growth of H. maydis colonies in vitro. The results of this study indicate that IAA compounds produced by bacteria are antifungal.
Aktivitas Antijamur dari Bakteri Penghasil Senyawa Imidazole-3-Oxide terhadap Jamur Helminthosporium Maydis Trisna Agung Phabiola; Ida Bagus Gde Pranatayana; Khamdan Khalimi
Nandur Vol 4 No 1 (2024)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

The fungus Helminthosporium maydis is a pathogen that causes leaf blight in corn plants.The use of biological agents is one way to control leaf blight that is environmentally friendly. One of these biological agents is bacteria that produce the compound imidazole-3-oxide. This research aims to determine the ability of bacteria producing imidazole-3-oxide compounds to inhibit the growth of H. maydis fungal colonies. The results showed that bacteria producing the compound imidazole-3-oxide were able to inhibit the growth of the fungus H. maydis in vitro with an inhibition percentage ranging from 94.74% to 95.52%. Bacterial filtrate producing imidazole-3-oxide compounds at a concentration of 20% is able to inhibit the growth of the fungus H. maydis with a percentage of inhibitory power ranging from 91.35% to 94.41% whereas at a concentration of 50% it was able to inhibit the growth of the H. maydis fungus with an inhibitory percentage of 100%. The results of this research provide new information that bacteria producing the compound imidazole-3-oxide can be used to control leaf blight disease in corn plants.