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All Journal Althea Medical Journal Biosaintifika: Journal of Biology & Biology Education
Daniel Joko Wahyono
Faculty of Biology, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java Rokhmani Rokhmani; Endang Ariyani Setyawati; Daniel Joko Wahyono
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.11720

Abstract

Protozoa species of Trichodina spp. may be found in most hatchery centers in Banyumas, Purbalingga, and Banjarnegara. However, the determination of Trichodina spp. types is still based on its body’s morphological variations, not yet molecular. A research has been conducted to identify molekuler of the Trichodina spp. with the infecting 18S rRNA gene marking on the gourami larvae in Exs. Residence of Banyumas, Central Java. The research used a survey method with the samples of gourami. Amplification of 18S rRNA gene from Trichodina heterodentata was Performed using PCR technique. Primer used is Forward primer (5 ‘-AAC CTG GTT GAT CCT GCC ATG-3’) and Reverse primer (5 ‘-TGA TCC TTC TGC AGG TTC ACC TAC-3’) which produces a 600 pb amplicon of DNA. Molecular research can be a complementary identification of organisms morphologically. Amplification of the partial 18S rRNA gene may be used to identify Trichodina specifically. Gourami larvae taken from the hatchery centers in Banyumas, Purbalingga, and Banjarnegara. The results show that the detected percentage of Trichodina heterodentata genes with the infecting 18S rRNA gene marking on the gourami larvae in Central Java taken from the hatchery centers in Banyumas, Purbalingga and Banjarnegara are respectively 10%, 10%, and 45%. This research provides a benefit in mapping the presence of protozoa pathogen of Trichodina spp. in gourami hatcheries in the Former Exs. Residence of Banyumas, Central Java
Cervical Cancer Chemoprevention Using Hippobroma longiflora Extract through Modulation of Ki-67 and p53 in Benzo[a]pyrene-Induced Wistar Rats Ana Bina Sari; Eman Sutrisna; Daniel Joko Wahyono
Althea Medical Journal Vol 13, No 2 (2026)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v13n2.4633

Abstract

Background: Cervical cancer remains one of the most common malignancies affecting women worldwide and is closely linked to dysregulated cell proliferation and impaired tumor suppressor responses. Herbs contain effective anticancer compounds that may be used alone or as adjuvants to existing chemotherapy regimens to improve efficacy and/or reduce drug-induced toxicity. Hippobroma longiflora (L.) G.Don a or kitolod is a medicinal plant known to contain antioxidant and anticancer compounds. This study evaluated the effect of ethanol extract of kitolod leaves on Ki-67 and p53 expression in a Benzo[a]pyrene (B[a]P)-induced cervical carcinogenesis model in female Wistar rats.Methods: This experimental study used a post-test-only control group design. Female Wistar rats (n=25) were grouped into five groups: a healthy control group without B[a]P exposure, a negative control group receiving only B[a]P (0.3 mg/kg BW/day); and three treatment groups receiving B[a]P combined with kitolod leaf extract at doses of 100, 200, or 300 mg/kg BW/day for 30 days. Phytochemical screening and gas chromatography–mass spectrometry (GC-MS) were used to characterize the extract. Cervical histopathologal and immunohistochemical examinations were performed to assess Ki-67 and p53 expression. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc tests, with p<0.05 considered statistically significant.Results: Compared with the negative control, kitolod leaf extract significantly reduced Ki-67 expression and increased p53 expression (p<0.001). The greatest effect was observed at the 300 mg/kg BW/day dose. Conclusions: Kitolod leaf ethanol extract modulates Ki-67 and p53 expression in B[a]P-exposed rats, suggesting anti-proliferative activity and enhancement of tumor suppressor responses during early cervical carcinogenesis.
Co-Authors Ana Bina Sari Eman Sutrisna Endang Ariyani Setyawati Rokhmani Rokhmani