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All Journal Biosaintifika: Journal of Biology & Biology Education
Herman Herman
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau
Agriculture, Biological Sciences & Forestry

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Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) Dewi Indriyani Roslim; Ashfira Ashfira; Dilla Mutiarawati; Tio Fanny Rosmeilinda; Nur Aisyah; Herman Herman; Wahyu Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.19052

Abstract

Some genes like 18S rRNA, 26S rRNA, elongation factor 1-alpha (EF1a), and beta-tubulin (TUB) are members of housekeeping genes group that are commonly used as internal control in gene expression study. This study aimed to isolate those four housekeeping genes of tuntun angin (Elaeocarpus floribundus). The research material included fresh leaves of E. floribundus that were picked up from Kajuik Lake in Riau Province and four primer pairs. The procedures consisted of total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), polymerase chain reaction (PCR), electrophoresis on 1% agarose gel, sequencing, and bioinformatic analysis. This study has been isolated 18S rRNA, 26S rRNA, EF1a, and TUB genes with the size of 422 bp, 922 bp, 856 bp, and 877 bp, respectively. The EF1a and TUB genes has never been reported in Elaeocarpaceae family. Thus, those partial DNA sequences are the first sequences reported from this species and can be used as reference genes in this plant after validation.
Isolation of Housekeeping Genes on Durik-durik (Syzygium sp) Dewi Indriyani Roslim; Liza Aulia Yusfi; Desriani Ritawati Hutagalung; Niswah Faizah; Lestari Andriani; Azrial Azrial; Herman Herman; Wahyu Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14234

Abstract

Housekeeping gene is a gene expressed with a fixed level and in abundant amounts under various conditions. After validation, the housekeeping gene can be used as an internal control to normalize gene expression data. This study reports the isolation of several housekeeping genes in Durik-durik plant (Syzygium sp). This plant material in form of fresh leaves from Durik-durik plants are taken from Kajuik Lake, Riau Province. The next stage is total DNA isolation, polymerase chain reaction, electrophoresis, sequencing and data analysis using bioinformatic tools. The isolated housekeeping genes included 18S rRNA, actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Beta-tubulin and Ubiquitin with sizes of 400 bp, 679 bp, 1134 bp, 836 bp, 1167 bp and 2155 bp, respectively. In addition to 18S rRNA, the five housekeeping genes are the first reported from the genus Syzygium and referable to isolate housekeeping genes in other species in this genus. The six housekeeping genes can be used as internal controls on Durik-durik plants after validation.
Co-Authors Ashfira Ashfira Azrial Azrial Desriani Ritawati Hutagalung Dewi Indriyani Roslim Dilla Mutiarawati Lestari Andriani Liza Aulia Yusfi Niswah Faizah Nur Aisyah Tio Fanny Rosmeilinda Wahyu Lestari