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All Journal Jurnal Bios Logos AL KAUNIYAH BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Unri Conference Series: Community Engagement Journal of Management Education Social Sciences Information and Religion
Wahyu Lestari
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Riau
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology

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THE DNA SEQUENCE ENCODING GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPC) ENZYME ON TUNTUN ANGIN PLANT (ELAEOCARPUS FLORIBUNDUS BI) Dewi Indriyani Roslim; Hestia Hairima; Herman Herman; Wahyu Lestari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (498.851 KB) | DOI: 10.31289/biolink.v7i1.3310

Abstract

Glyceraldehyde 3-phosphate dehydrogenase (GapC) is an enzyme involved in glycolysis. The expression of this gene tends to abundant in eukaryotic cells, so this gene is frequently used as an internal control in gene expression analysis. This research aims to isolate the DNA sequence of the GapC gene from tuntun angin (Elaeocarpus floribundus BI). Methods included a collection of the leaves from Kajuik Lake, Riau Province then the DNA extraction, electrophoresis, amplification of partial DNA sequence of GapC gene, cloning and sequencing. The DNA sequence was analyzed using the BLASTn program and MEGA6 software. The GapC sequence obtained in this study was 933 bp in size, consisting of four introns and five exons, and encoding 137 deduced amino acids. The BLASTn analysis showed that the sequence has 89.84%-90.16% similarity to other species of Cunoniaceae family, such as species from the genus of Spiraeanthemum and Codia. The parsial sequence of E. floribundus GapC gene was more resemble the one of Spiraeanthemum than Codia genus. The GapC sequence obtained in this study was the first reported from the Elaeocarpaceae family. This sequence has the opportunity to serve as an internal control after validation.
Optimasi Konsentrasi Ekstrak Alang-Alang (Imperata cylindrica L.) untuk Memacu Pertumbuhan dan Produksi Jagung Manis (Zea mays Saccharata Sturt) Mayta Novaliza Isda; Wahyu Lestari; Diana Agriani
Al-Kauniyah: Jurnal Biologi Vol 6, No 1 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/al-kauniyah.v6i1.2829

Abstract

Sweet corn (Zea mays saccharata Struts) a type of corn of high economic value, not only used for consumption also used to make syrup. Imperata is one of the weeds that interfere with crop cultivation, but efforts continue to be made use of weeds as an addition to soil organic matter. The study was conducted to determine the concentration of the extract Imperata (Imperata cylindrica L.) appropriate in the sweet corn growth and determine the concentration of extract proper Imperata in the growth and production. The research method is a Completely Randomized Design (CRD) with 9 treatments and 3 replicates. The results showed that treatment of Imperata extracts with varying concentrations significantly influenced the growth of plant height and leaf number. But not significantly to the growth of the leaf length, leaf width, stem diameter, number of rows per cob, number of seeds per row and plant dry weight. At a concentration of 200 g / 250 ml was able to reduce the vegetative and generative growth of sweet corn.
POTENSI JAMUR INDIGENUS RIAU (Penicillium sp.PN6) DAN Neptunia oleracea UNTUK BIOREMEDIASI OIL SLUDGE Wahyu Lestari; Atria Martina; Rodesia Mustika Roza; Imelda Wardani
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.863 KB) | DOI: 10.15408/kauniyah.v11i1.6350

Abstract

Abstrak Lumpur minyak bumi (oil sludge) memiliki kandungan kontaminan petroleum hidrokarbon terbesar, karenanya pengolahan limbah oil sludge perlu dilakukan agar tidak mencemari lingkungan. Penelitian yang menguji kemampuan jamur indigenus Riau (Penicillium sp. PN6) dan legum akuatik (Neptunia oleracea) dalam meremediasi senyawa hidrokarbon dengan pengukuran total petroleum hidrokarbon (TPH) pada limbah oil sludge telah dilakukan. Pertumbuhan N. oleracea dilakukan pada media limbah oil sludge dengan konsentrasi 25, 50, 75, dan 100%. Selanjutnya dilakukan pemberian inokulum Penicillium sp. PN6 ke dalam media pertumbuhan. Hasil penelitian menunjukkan bahwa persentase pertumbuhan N. oleracea adalah 95,58; 85,87; 25,55; dan 0%, berturut-turut pada limbah oil sludge 25, 50, 75, dan 100%. Terjadi penurunan konsentrasi TPH awal pada semua perlakuan di akhir pengamatan. Penicillium sp. PN6 meningkatkan degradasi TPH dari limbah oil sludge dibandingkan dengan kombinasi Penicillium sp. PN6 dengan N. oleracea, tetapi kombinasi Penicillium sp.PN6 dengan N. oleracea meningkatkan persentase penurunan TPH dari limbah oil sludge dibandingkan dengan menggunakan Penicillium sp. PN6 saja. Penicillium sp. PN6 dan kombinasinya dengan N. oleracea sama-sama mampu menurunkan pH limbah oil sludge.Abstract Oil sludge contains the largest contaminant of petroleum hydrocarbon that needs treatment to prevent its pollution into the environment. A study examining the ability of a Riau indigenous fungi (Penicillium sp. PN6) and an aquatic legume (Neptunia oleracea) to remediate hydrocarbon compounds by determining total petroleum hydrocarbon (TPH) in oil sludge waste has been conducted. The growth of N. oleracea was performed in the oil sludge waste media by concentrations of 25, 50, 75, and 100%. The medium was inoculated with Penicillium sp. PN6. The results showed that the percentage of growth of N. oleracea were 95.58; 85.87; 25.55; and 0%, in the oil sludge waste of 25, 50, 75, and 100%, respectively. There was a decrease in initial TPH concentration at all treatments at the end of the observation. Penicillium sp. PN6 increased more TPH degradation of the oil sludge waste compared to a combination of Penicillium sp. PN6 and N.oleracea, but the combination increased the decrease percentage of TPH compared to those that used Penicillium sp. PN6 only. Penicillium sp. PN6 and its combination with N. oleracea are both able to decrease pH of the oil sludge waste. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 6350 
Pengolahan Sagu menjadi mie prebiotik sebagai makanan fungsional dan upaya peningkatan ketahanan pangan di Desa Alai Selatan, Kecamatan Tebing Tinggi Barat, Kepulauan Meranti Atria Martina; Wahyu Lestari; Tetty Marta Linda; Saberina Hasibuan; Imelda Wardani
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.112-116

Abstract

Noodles are a processed food product favored by most Indonesians, generally made from wheat which must be imported. Kepulauan Meranti Regency is the largest producer of sago in Indonesia. One of the products made from sago that is widely produced in Meranti is sa.go noodle. Making noodles with local raw materials such as sago and sweet potato is an effort to improve food security. Traditional sago noodle has high carbohydrate and fiber content but still has disadvantages such as not having inulin content as a prebiotic, less antioxidant and a less attractive color, while purple sweet potato contains inulin as prebiotic components, high antioxidants, vitamins and minerals.The utilization of prebiotics insago noodle has multiple advantages since they improved probiotic growth in the body, sensory features and provide a more well-balanced nutritional composition as functional food. This community service program aims to training on the process of making prebiotic sago noodles to the community in Alai Selatan Village.This activities are carried out by course and practice methods.The results showed that the participants were interested in making prebiotic sago noodles because they had a more attractive features, more nutritious and had a delicious taste. Prebiotic sago noodles can be used as an effort to biodiversify sago noodle products to support food security and improve the community's economy.
Kebun Organik Di Masa Adaptasi Kebiasaan Baru Pandemi Covid-19 Dengan Memanfaatkan Sampah Rumah Tangga Rodesia Mustika Roza; Atria Martina; Hari Kapli; Bernadeta Leni Fibriarti; Wahyu Lestari
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.658-664

Abstract

The World Health Organization has designated Covid-19 as a pandemic that causes activities outside the home to be restricted. In order to maintain health during the pandemic, many people are starting to choose foodstuffs produced from organic farming. The purpose of this activity is to provide information and motivation to society for reuse household waste with appropriate technology to produce compost. This service activity was carried out in Air Dingin Village, Bukit Raya District, Pekanbaru. The initial stage conveys material about the importance of maintaining health during the pandemic by consuming organic vegetables and fruits grown in their respective yards. Furthermore, the practice of making compost using the direct-use compost method and composting in a composter pot. Composting that reuses household waste has succeeded in opening up insight into the importance of the community's role in personal and environmental health and the public's understanding that a healthy life doesn't have to be expensive. In this activity, the participants showed their enthusiasm and stated that they would practice and share their knowledge with others in making compost to overcome the problem of household waste. This activity is also beneficial to keep the environment clean, healthy and beautiful.
Germination Capacity of Helianthus annuus Less Seeds on Soil Media Contaminated by Waste Wahyu Lestari; Sujarwati; Atria Martina; Imelda Wardani; Daryono
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4821

Abstract

Used oil waste is often dumped on the ground around the workshop, causing soil pollution which can reduce the function of the soil. The most difficult contaminants in used oil waste to decompose are hydrocarbon compounds, which are highly toxic, polycyclic aromatic hydrocarbons (PAHs). The concentration of oil hydrocarbon contaminants in a sample is determined by measuring the total petroleum hydrocarbon (TPH) concentration. This study was conducted to determine the germination ability of Helianthus anuus, Less seeds on soil media contaminated with waste oil at different concentrations of Total Petroleum Hydrocarbon (TPH). The results showed that the concentration of TPH in the media could inhibit the speed of seed germination. Treatment with low concentration of TPH 2.7% in the media increased % germination, shoot and root length and sprout biomass compared to the medium/intermediate (8.00%) and high (12.26%) TPH treatments and controls. However, there was no significant difference between all treatments regarding germination time and shoot length, although in the low TPH treatment (2.70%) there was a tendency for germination time to be faster than the other treatments, as well as to shoot length which was longer than the other treatments. The higher TPH concentration in the media can inhibit germination time, germination percentage, shoot and root length and biomass. Morphological observations of the growth of sprouts showed an inhibition of root hair formation along with the high TPH content in the media, but all treatments did not affect the growth of the shoots.
TEKNOLOGI BIOPORI: SOLUSI KAWASAN RAWAN GENANGAN DAN EDUWISATA DI DESA WISATA ALAM SUNGAI MESJID KOTA DUMAI Atria Martina; Wahyu Lestari; Tetty Marta Linda; Ninik Nihayatul Wahibah; Vanda Julita Julita
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 7 No 1 (2023)
Publisher : Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas Kampus Limau Manis - Padang, Sumatera Barat Indonesia-25163

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/logista.7.1.21-25.2023

Abstract

Desa Sungai Mesjid memiliki keindahan alam dan budaya Melayu yang masih terjaga. Pembentukan Kelompok Sadar Wisata (POKDARWIS) Alam Sungai Mesjid membuat potensi Desa sebagai desa ekowisata mulai dikembangkan untuk meningkatkan perekonomian masyarakat. Peningkatan pembangunan di Dumai membuat berkurangnya daerah resapan air serta penanggulangan sampah yang kurang baik turut memberi dampak tergenangnya beberapa daerah di Desa ini. Masyarakat berkeinginan mewujudkan desa yang hijau minim dari sampah dan genangan air jika hujan namun terkendala dengan pengetahuan dan biaya. Edukasi dan pemberdayaan masyarakat dengan pembuatan biopori sebagai resapan air dan penghasil kompos akan dapat mengurangi genangan air hujan dan mengatasi sampah organik. Metode penerapan kegiatan berupa presentasi, diskusi kelompok, praktek pembuatan lubang biopori, pemberian peralatan serta monitoring keberlanjutan pasca kegiatan. Selama kegiatan pelatihan terlihat adanya motivasi dan kesadaran masyarakat akan manfaat biopori. Pembuatan biopori tetap berlanjut pasca kegiatan yang dilakukan oleh masyarakat secara mandiri dan menjadikan teknologi pembuatan biopori sebagai salah satu paket eduwisata Desa Alam Sungai Masjid, sebagai upaya edukasi kepada masyarakat luas dan daya tarik wisata. Kata kunci: biopori, desa wisata, eduwisata, genangan, kompos Abstract Sungai Mesjid has wonderful scenery and Malay culture that are still preserved. The development of the Alam Sungai Mesjid Tourism Awareness Community (POKDARWIS) made the potential of the village as an ecotourism in order to improve the community’s economy during the Covid-19 pandemic. The increase in recent development in Dumai has reduced water catchment areas and poor waste management, and also impacted the inundation of several areas in this village. The community wants to create a green village where is minimal from garbage and puddles when rain falls, but it is constrained by knowledge and costs. Education and empowerment of society by making biopore as a water catchment and producing compost will be able to reduce rainwater puddles and overcome organic waste. The method of implementing the activity is a presentation, group discussion, practice of making biopore holes, provision of equipment and post-activity monitoring of sustainability. During the training activities, it was seen there were motivation and public awareness of benefits biopore. The development of biopore continues after the activities carried out by the community independently and makes biopore technology as one of the edutourism packages of Alam Sungai Masjid Village, as an educational effort to wider community and tourist attraction. Keywords: biopore, tourism village, edutourism, puddle, compost
Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu) Dewi Indriyani Roslim; Ashfira Ashfira; Dilla Mutiarawati; Tio Fanny Rosmeilinda; Nur Aisyah; Herman Herman; Wahyu Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.19052

Abstract

Some genes like 18S rRNA, 26S rRNA, elongation factor 1-alpha (EF1a), and beta-tubulin (TUB) are members of housekeeping genes group that are commonly used as internal control in gene expression study. This study aimed to isolate those four housekeeping genes of tuntun angin (Elaeocarpus floribundus). The research material included fresh leaves of E. floribundus that were picked up from Kajuik Lake in Riau Province and four primer pairs. The procedures consisted of total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), polymerase chain reaction (PCR), electrophoresis on 1% agarose gel, sequencing, and bioinformatic analysis. This study has been isolated 18S rRNA, 26S rRNA, EF1a, and TUB genes with the size of 422 bp, 922 bp, 856 bp, and 877 bp, respectively. The EF1a and TUB genes has never been reported in Elaeocarpaceae family. Thus, those partial DNA sequences are the first sequences reported from this species and can be used as reference genes in this plant after validation.
Isolation of Housekeeping Genes on Durik-durik (Syzygium sp) Dewi Indriyani Roslim; Liza Aulia Yusfi; Desriani Ritawati Hutagalung; Niswah Faizah; Lestari Andriani; Azrial Azrial; Herman Herman; Wahyu Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14234

Abstract

Housekeeping gene is a gene expressed with a fixed level and in abundant amounts under various conditions. After validation, the housekeeping gene can be used as an internal control to normalize gene expression data. This study reports the isolation of several housekeeping genes in Durik-durik plant (Syzygium sp). This plant material in form of fresh leaves from Durik-durik plants are taken from Kajuik Lake, Riau Province. The next stage is total DNA isolation, polymerase chain reaction, electrophoresis, sequencing and data analysis using bioinformatic tools. The isolated housekeeping genes included 18S rRNA, actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Beta-tubulin and Ubiquitin with sizes of 400 bp, 679 bp, 1134 bp, 836 bp, 1167 bp and 2155 bp, respectively. In addition to 18S rRNA, the five housekeeping genes are the first reported from the genus Syzygium and referable to isolate housekeeping genes in other species in this genus. The six housekeeping genes can be used as internal controls on Durik-durik plants after validation.
Pengaruh Pemasaran melalui Media Sosial terhadap Keterlibatan Pelanggan pada Pembelian Produk Skintific Siska Veronica; Nur Atika Dewi; Rahmatul Dani; Virly Anita Adilla Hrp; Hilda Surtiani; Nurhamidah Nurhamidah; Wahyu Lestari; Devina Adelia Putri; Hardisem Syabrus
Journal of Management Education Social Sciences Information and Religion Vol 1, No 2 (2024): September 2024
Publisher : CV. Rayyan Dwi Bharata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57235/mesir.v1i2.3051

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh pemasaran media sosial terhadap keterlibatan pelanggan pada pembelian produk Skintific. Responden dalam penelitian ini adalah mahasiswa Universitas Riau yang berjumlah 81 orang. Teknik pengumpulan data dilakukan dengan menggunakan kuesioner untuk mendapatkan data tentang pengaruh pemasaran media sosial terhadap keterlibatan pelanggan pada pembelian produk Skintific. Data yang terkumpul kemudian ditabulasi dengan bantuan excel dan dianalisis menggunakan bantuan SPSS versi 25. Hasil penelitian menunjukkan pemasaran melalui media sosial media sosial berpengaruh positif dan signifikan terhadap keterlibatan pelanggan pada pembelian produk Skintific.
Co-Authors Ashfira Ashfira Atria Martina Azrial Azrial Daryono Desriani Ritawati Hutagalung Devina Adelia Putri Dewi Indriyani Roslim Diana Agriani Dilla Mutiarawati Hardisem Syabrus Hari Kapli Herman Herman Herman Herman Hestia Hairima Hilda Surtiani Imelda Wardani L. FIBRIARTI, BERNADETA Lestari Andriani Lidya Putri Ande Siregar Liza Aulia Yusfi Mayta Novaliza Isda Ninik Nihayatul Wahibah Niswah Faizah Nur Aisyah Nur Atika Dewi Nurhamidah Nurhamidah Rahmatul Dani Rodesia Mustika Roza Saberina Hasibuan Siska Veronica Sujarwati Sujarwati Tetty Marta Linda Tio Fanny Rosmeilinda Vanda Julita Julita Virly Anita Adilla Hrp