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All Journal Biosaintifika: Journal of Biology & Biology Education Journal of Biodiversity and Biotechnology
Ari Susilowati
Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry

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Isolation and Identification of α-glycosidase Inhibitor Producer Endophytic Bacteria from Salak Pondoh Fruit (Salacca edulis) Ari Susilowati; Citra Praba Yunita Dewi; Siti Lusi Arum Sari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.21031

Abstract

Alpha-glycosidase inhibitors can delay the hydrolysis of oligosaccharide and disaccharide into glucose, which can prevent or treat hyperglycemia in diabetes mellitus. The rind and flesh of Salak Pondoh fruit are known to produce α-glycosidase inhibitor compound. Endophytic bacteria that live in plant tissues potentially produce compounds such as in host plants. Exploration of endophytic bacteria from the rind and flesh of Salak Pondoh is one of the efforts to obtain isolates of bacteria producing α-glycosidase inhibitors. The objective of this study is to isolate and identify endophytic bacteria producing inhibitor α-glycosidase from rind and flesh of Salak Pondoh, and to know the activity of its α-glycosidase inhibitor. Isolation of endophytic bacteria was done by inoculating surface-sterilized plant samples on Nutrient Agar (NA) medium. The inhibitory activity towards α- glycosidase analysis was performed using the spectrophotometric method (λ = 415 nm), with p-nitro phenyl α-D-glucopyranoside as the substrate. Identification of the bacteria was performed based on the 16S rRNA gene sequence.  The sequencing was done at 1st Base Singapore and the obtained sequences were identified using the BLAST Nucleotide device on the NCBI website. In total, 6 bacterial isolates were obtained. The inhibitory activity ranged from 6.14-62.95% and the highest was generated by Kt-E isolates. The bacteria obtained were isolated Dt- A and Dt-B that represent genus Xanthomonas, Kt-E from genus Paenibacillus, Kt-I from genus Bacillus, Dm-A1 and Dm-A2 from family Enterobacteriaceae. The results confirm the potential of the endophyte bacteria of Salak Pondoh to be an alternative source of hyperglycemia medication.
Isolation of Lactic Acid Bacteria from Soil and Its Antifungal Activity Against Aspergillus flavus Athiyyah Jihan Tifani; Tjahjadi Purwoko; Ari Susilowati
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.109898

Abstract

Food stocks, such as grains, fruits, and vegetables are vulnerable to spoilage by Aspergillus flavus, which typically controlled using chemical preservatives. Concerns about the health impact of these chemicals highlight the need for safer alternatives, such as biopreservatives. Lactic acid bacteria (LAB) are Generally Recognized as Safe (GRAS) microorganisms and have potential application as biopreservative agent, because LAB produced various metabolites with antifungal activities. LAB could be found in nutrient-rich environments, including rhizosphere soil and poultry house soil due to the nutrient residues for growth. The aims of this study were to isolate LAB from soils and to evaluate their antifungal activity with a focus on potential applications as biopreservatives. The LAB isolates from soil were characterized and screened for antifungal activity using the dual culture method. The LAB isolates inhibited A. flavus growth with the largest growth inhibition was showed by LAB isolate 1 that isolated from poultry house soil (17.65%). However, statistics analysis showed that fungal growth inhibition by LAB isolates were not significantly difference (p>0,05). Although the result was not statistically significant, all  LAB isolates significantly inhibited conidia development. This showed potential application of LAB isolates as biopreservative agent. Keywords: antifungal, Aspergillus flavus, biopreservative, food stock, lactic acid bacteria
Co-Authors Athiyyah Jihan Tifani Citra Praba Yunita Dewi Siti Lusi Arum Sari Tjahjadi Purwoko