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All Journal Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Bioscientist : Jurnal Ilmiah Biologi Journal of Biodiversity and Biotechnology
Ari Susilowati
Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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 1 
Isolation and Identification of α-glycosidase Inhibitor Producer Endophytic Bacteria from Salak Pondoh Fruit (Salacca edulis) Ari Susilowati; Citra Praba Yunita Dewi; Siti Lusi Arum Sari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.21031

Abstract

Alpha-glycosidase inhibitors can delay the hydrolysis of oligosaccharide and disaccharide into glucose, which can prevent or treat hyperglycemia in diabetes mellitus. The rind and flesh of Salak Pondoh fruit are known to produce α-glycosidase inhibitor compound. Endophytic bacteria that live in plant tissues potentially produce compounds such as in host plants. Exploration of endophytic bacteria from the rind and flesh of Salak Pondoh is one of the efforts to obtain isolates of bacteria producing α-glycosidase inhibitors. The objective of this study is to isolate and identify endophytic bacteria producing inhibitor α-glycosidase from rind and flesh of Salak Pondoh, and to know the activity of its α-glycosidase inhibitor. Isolation of endophytic bacteria was done by inoculating surface-sterilized plant samples on Nutrient Agar (NA) medium. The inhibitory activity towards α- glycosidase analysis was performed using the spectrophotometric method (λ = 415 nm), with p-nitro phenyl α-D-glucopyranoside as the substrate. Identification of the bacteria was performed based on the 16S rRNA gene sequence.  The sequencing was done at 1st Base Singapore and the obtained sequences were identified using the BLAST Nucleotide device on the NCBI website. In total, 6 bacterial isolates were obtained. The inhibitory activity ranged from 6.14-62.95% and the highest was generated by Kt-E isolates. The bacteria obtained were isolated Dt- A and Dt-B that represent genus Xanthomonas, Kt-E from genus Paenibacillus, Kt-I from genus Bacillus, Dm-A1 and Dm-A2 from family Enterobacteriaceae. The results confirm the potential of the endophyte bacteria of Salak Pondoh to be an alternative source of hyperglycemia medication.
Isolation of Lactic Acid Bacteria from Soil and Its Antifungal Activity Against Aspergillus flavus Athiyyah Jihan Tifani; Tjahjadi Purwoko; Ari Susilowati
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.109898

Abstract

Food stocks, such as grains, fruits, and vegetables are vulnerable to spoilage by Aspergillus flavus, which typically controlled using chemical preservatives. Concerns about the health impact of these chemicals highlight the need for safer alternatives, such as biopreservatives. Lactic acid bacteria (LAB) are Generally Recognized as Safe (GRAS) microorganisms and have potential application as biopreservative agent, because LAB produced various metabolites with antifungal activities. LAB could be found in nutrient-rich environments, including rhizosphere soil and poultry house soil due to the nutrient residues for growth. The aims of this study were to isolate LAB from soils and to evaluate their antifungal activity with a focus on potential applications as biopreservatives. The LAB isolates from soil were characterized and screened for antifungal activity using the dual culture method. The LAB isolates inhibited A. flavus growth with the largest growth inhibition was showed by LAB isolate 1 that isolated from poultry house soil (17.65%). However, statistics analysis showed that fungal growth inhibition by LAB isolates were not significantly difference (p>0,05). Although the result was not statistically significant, all  LAB isolates significantly inhibited conidia development. This showed potential application of LAB isolates as biopreservative agent. Keywords: antifungal, Aspergillus flavus, biopreservative, food stock, lactic acid bacteria
Optimization of Biological Delignification of Sugar Palm (Arenga pinnata) Stems Using Streptomyces griseorubens for Bioethanol Production Nurul Hidayah; Ari Susilowati; Sri Sugiwati; Amaliyah Rohsari Indah Utami
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 3 (2026): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i3.22366

Abstract

This study aimed to optimize the biological pretreatment of sugar palm (Arenga pinnata) stem biomass using Streptomyces griseorubens through Response Surface Methodology (RSM), based on variations in pH, substrate loading, and incubation time. The lignocellulosic composition of the biomass was analyzed using the NREL method, while process optimization was performed using Design-Expert® version 13 with a Central Composite Design (CCD) comprising 20 experimental runs. Ligninolytic enzyme activities, including lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase, were evaluated. The pretreated biomass was subsequently subjected to saccharification followed by fermentation with Saccharomyces cerevisiae using the Separate Hydrolysis and Fermentation (SHF) approach. ANOVA showed that the model was significant for lignin degradation (p = 0.012 < 0.05). The optimum conditions were obtained at a substrate loading of 9 g, pH 4, and an incubation period of 21 days, resulting in 12.45% lignin degradation. Under these conditions, the cellulose content increased from 34.22% to 41.99%, and the hemicellulose content increased from 8.18% to 22.99%, whereas the lignin content decreased from 34.03% to 30.86%. Laccase, MnP, and LiP activities reached 11.32, 96.69, and 187.26 U/mL, respectively. Subsequent saccharification produced 1.847 g of reducing sugars from 5 g of biomass after 48 h, while fermentation yielded an ethanol concentration of 0.97% (w/w) after 72 h. These findings demonstrate the potential of S. griseorubens-mediated biological pretreatment to enhance the conversion of sugar palm stem biomass for second-generation bioethanol production.
Relationship between altitudes, morphological traits, and biochemical compositions of Coffea canephora Pierre ex A. Froehner in Temanggung, Indonesia Intan Widya Pangestika; Ari Susilowati; Edi Purwanto; Gunawan
E-Journal Menara Perkebunan Vol. 93 No. 1 (2025): 93(1), 2025
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i1.621

Abstract

Among various edaphoclimatic factors influencing coffee bean quality, altitude is one of the primary factors that should be taken into account. This study aims to explore the relationship between altitude, morphological traits, and biochemical composition of robusta coffee in Temanggung Regency, the largest coffee-producing area in Central Java Province, Indonesia. Research sites were purposively selected and categorized into two altitude groups: GS, PS, and GN, located at circa 600 m a.s.l., and GT, WO, and TG, located at circa 900 m a.s.l. A total of 15 morphological traits were observed, consisting of eight vegetative and seven generative traits. Caffeine content was measured following the AOAC procedure using UV-Vis spectrophotometry, while brew acidity was evaluated using a pH meter. Several morphological traits of Temanggung robusta coffee demonstrated significant relationships with altitude. Canopy and stem diameter showed negative correlations with altitude, with correlation coefficients of -0.366 and  -0.408, respectively. Conversely, fruit width               (r = 0.041), bean length (r = 0.049), and bean thickness (r = 0.047) exhibited positive correlations. Regarding biochemical composition, caffeine content stood out by displaying a strong positive correlation with altitude (r = 0.816). Additionally, several morphological traits, including the number of primary branches, the number of productive branches, the number of fruits per bunch, and traits related to fruits and beans, appear to be advantageous for selection and breeding programs. Understanding these relationships provides valuable insights for developing superior Temanggung robusta coffee plants adapted to specific altitudinal conditions.
Co-Authors Amaliyah Rohsari Indah Utami Athiyyah Jihan Tifani Citra Praba Yunita Dewi Edi Purwanto Gunawan Intan Widya Pangestika Nurul Hidayah Siti Lusi Arum Sari Sri Sugiwati Tjahjadi Purwoko