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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Natur Indonesia Jurnal Natur Indonesia
Mohamad Gazali
Prodi Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan Universitas Teuku Umar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Physics

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Evaluasi penangkapan radikal bebas DPPH ekstrak daun Ipomoea pes-caprae Linn asal Pantai Labuhan Haji, Aceh Selatan: The evaluation of DPPH free radical scavenging of Ipomoea pes-caprae leaf from the Beach of Labuhan Haji, South Aceh Mohamad Gazali; Hayatun Nufus; Rina Syafitri; Muhammad Ali Sarong; Shafira Ananda Widya Fadly
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 2 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i2.43795

Abstract

Tumbuhan Ipomoea pes-caprae merupakan tumbuhan herba yang tumbuh pada substrat berpasir di mana sudah dimanfaatkan oleh masyarakat pesisir sebagai obat tradisional untuk menyembuhkan berbagai penyakit. Tujuan penelitian ini adalah untuk mengevaluasi aktivitas penangkapan radikal bebas pada ekstrak daun I. pes-caprae yang berasal dari Pantai Labuhan Haji, Aceh Selatan. Penelitian meliputi ekstraksi menggunakan pelarut metanol, etil asetat dan n-heksana, uji fitokimia, aktivitas penangkapan radikal bebas DPPH, dan kandungan total fenolik. Hasil penelitian menunjukkan bahwa daun I. pes-caprae memiliki rendemen tertinggi pada ekstrak metanol 6,5% yang diikuti dengan ekstrak n-heksana 3,5% dan ekstrak etil asetat 1,6%. Ekstrak daun tumbuhan I. pes-caprae mengandung senyawa alkaloid, fenolik, saponin, flavonoid, dan tanin. Hasil aktivitas penangkapan radikal bebas menunjukkan bahwa ekstrak metanol memiliki nilai IC50 sebesar 13,83±5,85 mg/L sedangkan ekstrak etil asetat nilai IC50 sebesar 7,36±1,91 mg/L dan ekstrak n-heksana memiliki nilai IC50 16,01±3,89 mg/L, asam askorbat dengan nilai IC50 sebesar 3,5±1,74 mg/L sebagai kontrol positif. Kandungan total fenolik daun I. pes-caprae ekstrak etil asetat sebesar 22,9±0,64 mg GAE/g sedangkan ekstrak metanol sebesar 9,89±2,62 mg GAE/g dan kadar total fenolik paling rendah terdapat pada ekstrak n-heksana dengan nilai sebesar 5,90±2,67 mg GAE/g. Kadar fenolik memiliki korelasi positif dengan aktivitas antioksidan yang diindikasikan bahwa aktivitas antioksidan dari ekstrak daun I. pes-caprae didonorkan oleh senyawa fenolik sedangkan sisanya dipengaruhi oleh senyawa lain yang terkandung dalam ekstrak daun I. pes-caprae.
Inhibition Zone Test of Chaetomorpha sp. Extract Against Aeromonas hydrophilla and Vibrio sp. Bacteria Sri Wahyuni; Mohamad Gazali; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 20 No. 2 (2022): October
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Chaetomorpha sp. is a green algae that has anti-bacterial compounds and at certain times found very much. The purpose of this study was to see the inhibition zone produced from the ethanol extract of Chaetomorpha sp. against A. hydrophilla and Vibrio sp. This research was done in March 2022. Extraction of Chaetomorpha sp., antibacterial effectiveness testing was done at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Riau University. The method used is descriptive method. Sensitivity test was performed using the Kirby Bauer disc method. To reduce the error rate, it was repeated three times. The doses of macroalgae extract used were, doses of 100% (10,000 ppm), 90% (9,000 ppm), 80% (8,000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline). Based on the observations, it was found that the use of ethanol extract of Chaetomorpha sp. The result is able to inhibit the growth of bacteria A. hydrophilla and Vibrio sp. at the highest dose (10000 ppm) in an range of 13.5 -14.5 mm which was classified as strong category, the lowest dose was at 5000 ppm as moderate category.
Inhibition Zone Test of Chaetomorpha sp. Extract Against Aeromonas hydrophilla and Vibrio sp. Bacteria Sri Wahyuni; Mohamad Gazali; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 20 No. 2 (2022): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.20.2.50-54

Abstract

Chaetomorpha sp. is a green algae that has anti-bacterial compounds and at certain times found very much. The purpose of this study was to see the inhibition zone produced from the ethanol extract of Chaetomorpha sp. against A. hydrophilla and Vibrio sp. This research was done in March 2022. Extraction of Chaetomorpha sp., antibacterial effectiveness testing was done at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Riau University. The method used is descriptive method. Sensitivity test was performed using the Kirby Bauer disc method. To reduce the error rate, it was repeated three times. The doses of macroalgae extract used were, doses of 100% (10,000 ppm), 90% (9,000 ppm), 80% (8,000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline). Based on the observations, it was found that the use of ethanol extract of Chaetomorpha sp. The result is able to inhibit the growth of bacteria A. hydrophilla and Vibrio sp. at the highest dose (10000 ppm) in an range of 13.5 -14.5 mm which was classified as strong category, the lowest dose was at 5000 ppm as moderate category.
Co-Authors Hayatun Nufus Muhammad Ali Sarong Rina Syafitri Ronal Kurniawan Shafira Ananda Widya Fadly Sri Wahyuni