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All Journal Mukhtabar: Journal of Medical Laboratory Technology Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat Laboratory Journal of Infectious Diseases
Euis Tia Istianah
STIKes Muhammadiyah Ciamis
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Description of Candida albicans Fungus in The Oral Mucosa of Patients With Fe Deficiency Anemia in RSUD Ciamis Aditya Nur Romadhon; Mei Widiati; Undang Ruhimat; Euis Tia Istianah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.217

Abstract

Background & Objective Indonesia is a developing country where Fe deficiency anemia is common. Fe deficiency anemia is a malnutrition disease caused by a lack of Fe in the patient's body. Fe deficiency anemia is triggered by a lack of Fe in the body for the formation of hemoglobin, causing patients to experience blood deficiency and thinning of oral mucosal epithelial cells which can cause oral candidiasis. Analyzing the description of oral candidiasis in patients with Fe deficiency anemia. Methods  This study is a descriptive study that recruited a total sampling of 17 patients with Fe deficiency anemia. Oral candidiasis in Fe deficiency anemia was measured by planting on sabouraud dextrose agar (SDA) media and sugars media then observed macroscopically and microscopically from SDA and sugars cultures using a microscope. Results Based on the results of the study, 15 (88%) positive respondents and 2 (12%) negative respondents. Conclusion The results of the examination of oral candidiasis as many as 15 samples were affected by oral candidiasis caused by Candida albicans.
Overview of Hepatitis B Surface Antigen Examination Results in Health Analyst Study Program Students of STIKes Muhammadiyah Ciamis Epi Amalia; Atun Farihatun; Euis Tia Istianah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.363

Abstract

Background & Objective: Hepatitis B can also be called a liver infection caused by the Hepatitis B Virus (VHB). Health workers are at high risk of being infected with the Hepatitis B Virus, this is due to a lack of knowledge and skills such as the use of Personal Protective Equipment and not implementing health protocols which can cause exposure to the Hepatitis B Virus. This study aims to determine the description of the results of the Hepatitis B surface Antigen examination in Health Analyst Study Program Students of STIKes Muhammadiyah Ciamis.  Method: This study is a descriptive study with a purposive sampling technique conducted in May 2023. Respondents in this study were students of STIKes Muhammadiyah Ciamis as many as 38 respondents with measurements using the Sandwich method ELISA which then the results were processed manually and displayed in tabular format for narration. Result: The results of the Hepatitis B Surface Antigen examination showed that all respondents were 100% negative for Hepatitis B. Conclusion: The research that has been carried out, shows that all respondents, namely students of the Health Analyst Study Program at STIKes Muhammadiyah Ciamis, are not exposed to the Hepatitis B virus.
Edukasi Zat Pewarna Pangan Berbahaya Di SMA Negeri 1 Sukadana Doni Setiawan; Rivana Ariyadi; Euis Tia Istianah; Aldi Febriansyah; Amanda Tyas Zaharani; Chintya Nur Aeni; Desry Delia Futri; Dinda Putri Stevani; Firdaus Nur Rohman; Hayfa Azkal Azkiya; levi Ayu Ramadhanty; Mitha Silvyana; Nailah Ghaissani Dwi Gustari; Nuraisyah Kurniawan; Putri Laksmi Margarethi; Risma Risma; Taopik Cahyadi
Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat Vol. 1 No. 2 (2023): Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat (Juli-Desember 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/daipkm.v1i2.410

Abstract

Introduction: In general, food coloring can be obtained from natural dyes and safe synthetic food dyes. Natural dyes are sometimes considered less stable and less durable than synthetic food dyes. Although there are synthetic dyes that are safe for food, there are still people who use materials that are not suitable for use in food. The use of harmful food dyes must be monitored and recognized for their existence. Harmful food dyes that are commonly found are textile dyes such as Rhodamine B or methanyl yellow. Because it is not a proper food coloring, consumption of foods containing this ingredient can cause a series of health problems for those who consume it, especially in a relatively long period of time. This education aims to allow students to distinguish foods that use natural coloring agents and artificial coloring agents. The results of this activity students can find out the characteristics of foods that use harmful dyes and know the prevention that can be done. Objective: The purpose of this service is for SMAN 1 SUKADANA students to know the dangers of using hazardous coloring agents in food. Method: The method used is providing education by providing material to students, tests before and after material on the Use of Harmful Coloring Substances in Food. Result: Increased knowledge and attitudes of students are tested through pre-test and post-test. There has been an increase in knowledge in students about the dangers of using harmful dyes in food in general by 20%, so that the realization of the purpose of this service is expected to be able to distinguish food ingredients that contain harmful coloring agents in food and those that do not contain harmful coloring agents in food. Basically, knowledge and attitudes also depend on the high or low level of education of students. Therefore, the existence of this counseling can help students in adding insight into the dangers of using harmful dyes. The implementation of this activity can run well due to positive support in the implementation process, where support from various parties and there are no obstacles whatsoever. Conclusion: The socialization activity on the use of dangerous coloring substances in food to students at SMAN 1 Sukadana received a good response from the school and also students. With outreach activities regarding this, students will understand and be more careful in choosing food to avoid these chemicals.
Cytological Characteristics of Pleural Effusion with Variations in 96% Alcohol Fixation Time Using Papanicolaou Staining Priska Alicia Vansugisti; Ary Nurmalasari; Euis Tia Istianah; Rahayu Nirmatul Mutmainah
Laboratory Journal of Infectious Diseases Vol. 2 No. 1 (2026): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

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Abstract

Pleural effusion is a clinical condition that may indicate serious underlying diseases, including infection, malignancy, and systemic disorders. Cytological examination of pleural fluid plays an important role in determining the possible etiology of pleural effusion and requires proper fixation to preserve cellular morphology and staining quality. This study aimed to evaluate the cytological characteristics and preparation quality of pleural fluid specimens fixed in 96% alcohol for 15 minutes and 30 minutes using the Papanicolaou staining method. This quasi-experimental study included 16 pleural fluid samples obtained from routine laboratory examinations. Each sample was processed into four cytological smears, consisting of two duplicate smears fixed in 96% alcohol for 15 minutes and two duplicate smears fixed in 96% alcohol for 30 minutes. A total of 64 smears were evaluated, with 32 smears in each fixation group. All smears were stained using the Papanicolaou method and assessed microscopically based on background appearance, cellular morphology, nuclear characteristics, and overall staining quality. Of the 32 smears fixed for 15 minutes, 20 smears (62,5%) were classified as poor quality and 12 smears (37,5%) as good quality. In contrast, among the 32 smears fixed for 30 minutes, 3 smears (9,4%) were classified as poor quality and 29 smears (90,6%) as good quality. These findings indicate that fixation using 96% alcohol for 30 minutes produces better cytological preparation quality than fixation for 15 minutes. Therefore, a 30-minute fixation period is recommended to support optimal cytological evaluation and diagnostic accuracy.
Profile of Anti-Helicobacter pylori IgG Antibody Test Results in Patients with Chronic Gastritis at the Baregbeg Community Health Center Silma Yuniar; Atun Farihatun; Euis Tia Istianah; Rahayu Nirmatul Mutmainah
Laboratory Journal of Infectious Diseases Vol. 2 No. 1 (2026): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

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Abstract

Gastritis is an inflammation of the gastric mucosa that may be caused by various factors, including irregular dietary habits, nonsteroidal anti-inflammatory drug (NSAID) use, psychological stress, alcohol consumption, and Helicobacter pylori infection, which is recognized as a major cause of chronic gastritis. In primary healthcare settings, identification of the underlying etiology of gastritis is often limited by the availability of diagnostic laboratory facilities. Therefore, serological detection of anti-Helicobacter pylori immunoglobulin G (IgG) antibodies may serve as an alternative diagnostic approach. This study aimed to determine the serological profile of anti-Helicobacter pylori IgG antibodies among patients with chronic gastritis attending the Baregbeg Community Health Center. A descriptive study was conducted involving 24 outpatients diagnosed with chronic gastritis who met the inclusion criteria. Participants were recruited using a total sampling technique. Serum samples were collected and analyzed using an immunochromatographic assay at the Baregbeg Community Health Center Laboratory between April and June 2025. All 24 participants (100%) tested negative for anti-Helicobacter pylori IgG antibodies. No serological evidence of Helicobacter pylori infection was detected among the study participants. In conclusion, no serological evidence of Helicobacter pylori infection was detected among chronic gastritis patients included in this study, with an observed Helicobacter pylori seropositivity rate of 0%.
Screening for Anemia Through Hemoglobin (Hb) Tests in Female Students at STIKes Muhammadiyah Ciamis Euis Tia Istianah; Rivana Ariyadi; Crystia Dwimulyani
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 2 (2025): Mukhtabar: Journal of Medical Laboratory Technology (October 2025)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i2.791

Abstract

Background & Objectives: Indonesia ranked 5th in the world (22.331%) in terms of anemia incidence in 2019, after Pakistan (22.409%), Nigeria (25.475%), China (54.041%), and India (187.325%). The prevalence of anemia in Indonesia based on data from the 2018 Basic Health Research was 48.9%. Globally, the prevalence of anemia is higher in adolescent girls than in adolescent boys. Anemia is a disease caused by a lack of red blood cells. This study aims to determine anemia screening through hemoglobin testing in female students at STIKes Muhammadiyah Ciamis. Method: The method used in this study was descriptive, with the respondents being female students at STIKes Muhammadiyah Ciamis. Hemoglobin levels were measured using a spectrophotometer with the synehemoglobin method. This study was conducted at the Hematology Laboratory of STIKes Muhammadiyah Ciamis in May 2024. Results: Based on the examination results of 42 samples, 1 person (2%) was found to have moderate anemia, 4 people (10%) had mild anemia, and 37 people (88%) had normal results. Conclusion: The average hemoglobin level of female students at STIKes Muhammadiyah Ciamis is normal.
Co-Authors Aditya Nur Romadhon Aldi Febriansyah Amanda Tyas Zaharani Ary Nurmalasari Atun Farihatun Chintya Nur Aeni Crystia Dwimulyani Desry Delia Futri Dinda Putri Stevani Doni Setiawan Epi Amalia Firdaus Nur Rohman Hayfa Azkal Azkiya levi Ayu Ramadhanty Mei Widiati Mitha Silvyana Nailah Ghaissani Dwi Gustari Nuraisyah Kurniawan Priska Alicia Vansugisti Putri Laksmi Margarethi Rahayu Nirmatul Mutmainah Risma Risma Rivana Ariyadi Silma Yuniar Taopik Cahyadi Undang Ruhimat