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Indeks Formula Eritrosit Untuk Uji Skrining Talasemia Beta Minor Doni Setiawan; Hendri Setiawan; Ary Nurmalasari
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.247

Abstract

Erythrocyte indices have been used as the first indicator in screening for beta-thalassemia minor. However, iron deficiency in hemoglobinopathies can reduce the sensitivity of screening, and the spectrum of mutations of different hemoglobinopathies between populations will result in discontinuation of the results of different erythrocyte indices. The research objective in this study was to determine the description of the erythrocyte indices used to screen for beta-thalassemia trait in Ciamis. The research method used in this study was a quantitative descriptive, using a cross-sectional study, data collection had been done during March-May 2021. The subjects of this study were 104 parents of a beta-thalassemia trait who have children with a beta-thalassemia major in Ciamis. The examination parameters used were complete blood count, with processing twenty-six erythrocyte formula index values. Ninety-two respondents were included in the inclusion criteria. The results of the erythrocyte indices in beta-thalassemia trait are England and Fraser, RBC, Mentzer, Srivastava, Shine and Lal, Ricerca, Green and King, Das Gupta, Jayabose, Telmissani-MCHD, Telmissani-MDH, Huber-Herklotz, Kerman I, Kerman II, Sirdah, Ehsani, Keikhaei, Nishad, Wongprachum, Sehgal, Pornprasert, Sirachainan, Bordbar, Matos and Carvalho and CRUISE has cut-off value in that range according to previous studies. Only the Bessman erythrocyte indices has a value of 17, exceeding the cut-off. Twenty-five erythrocyte indices with cut-off value according to previous studies can be used for beta-thalassemia screening in Ciamis, except for the Bessman erythrocyte indices.
Health Promotion with Counseling on Fulfilling Balanced Nutritional Needs for Community Groups in Pandemic Covid 19 Outbreak Nurhidayat Nurhidayat; Suhanda Suhanda; Doni Setiawan; Heri Ariyanto; Henri Setiawan
ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 4 No. 2 (2021): ABDIMAS UMTAS: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Muhammadiyah Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.606 KB) | DOI: 10.35568/abdimas.v4i2.1424

Abstract

Covid-19 pandemic could increase forms of malnutrition such as overweight and micronutrient deficiencies. Fruits and vegetables are the best sources of vitamins and minerals. The content of vitamins and minerals can act as antioxidant compounds. The method used in this service activity consists of the planning stage, the implementation of the activity, and ends with an evaluation as a measure of the level of success. Health education is carried out by means of lectures and question and answer sessions, educational media using leaflets and PowerPoint Presentations. Results: the evaluation shows that the activities have been carried out well in accordance with the results indicators and criteria on the Evaluation Sheet instrument. The sheet is based on five dimensions, namely Punctuality and Duration, Participation, Knowledge, Job Description and Problem Solving. The content of the evaluation is carried out by two independent observers.
Skrining Talasemia Beta Minor pada Pelajar SMK di Kecamatan Ciamis Doni Setiawan; Atun Farihatun; Ary Nurmalasari
Jurnal Kesehatan Vokasional Vol 5, No 3 (2020): Agustus
Publisher : Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkesvo.57621

Abstract

Latar Belakang: Frekuensi talasemia beta minor di Indonesia mencapai 6–10%. Talasemia beta minor bersifat asimtomatik sehingga peran deteksi dini perlu dilakukan untuk mencegah kelahiran talasemia mayor. Pemeriksaan hematologi telah diketahui dan teruji dapat digunakan untuk skrining talasemia beta minor pada populasi beresiko. Meningkatnya penderita talasemia beta mayor di Kabupaten Ciamis dikarenakan belum maksimalnya skrining dilakukan. Tujuan: Mengetahui frekuensi talasemia beta minor pada pelajar SMK di Kecamatan Ciamis. Metode: Penelitian ini menggunakan metode deskriptif kuantitatif, dengan teknik cross‑sectional, yang telah dilakukan sejak bulan Maret‑Juni 2020. Subjek penelitian adalah pelajar SMK di Kecamatan Ciamis. Parameter pemeriksaan yang digunakan untuk skrining talasemia beta minor adalah MCV, Indeks Men3er, Indeks RDW, dan OTOFT. Hasil: Dari seluruh pelajar yang mengisi kuesioner sebanyak 238 pelajar, hanya 60 pelajar yang bersedia dan memenuhi kriteria penelitian. Hasil pemeriksaan parameter skrining talasemia beta minor didapatkan 5 (8,3%) pelajar MCV < 80 fL, 3 (5%) pelajar Indeks Men8er <13, Indeks RDW < 220 6 (10%) pelajar dan 9 (15%) pelajar dengan OTOFT positif. Kesimpulan: Pada penelitian ini ditemukan 3 (5%) pelajar sebagai talasemia beta minor.
HASIL PEMANTAPAN MUTU INTERNAL PEMERIKSAAN HbA1c Doni Setiawan Doni Setiawan
JURNAL KESEHATAN : JURNAL ILMU- ILMU KEPERAWATAN, KEBIDANAN, FARMASI DAN ANALIS KESEHATAN, SEKOLAH TINGGI KESEHATAN MUHAMMADIYAH CIAMIS Vol. 7 No. 2 (2020): Oktober 2020
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v7i2.94

Abstract

HbA1c is a small component of hemoglobin that binds to blood sugar. Quality Control is usually done by examining the control material that has known the range value and comparing the results of the inspection tool with the range of the control material. HPLC (High Performance Liquid Chromatography) is an analytical chemical technique used to separate components in a mixture. The purpose of this study was to determine the Internal Quality Stabilization on HbA1c examination using Hb Analyzer. The method used is descriptive. The technique of collecting data uses primary data. Data is obtained by conducting control material checks during the control period which is carried out for 26 days and the data is processed computerically then plotted into the graph. Based on research in May-June 2019 good results were obtained, where not deviant
EDUKASI PENYAKIT THALASEMIA PADA MAHASISWA STIKES MUHAMMADIYAH CIAMIS Doni Setiawan; Henri Setiawan; Ary Nurmalasari
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 6, No 3 (2022): September
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v6i3.9655

Abstract

ABSTRAKPrevalensi penyakit thalasemia di Indonesia, seperti di Kabupaten Ciamis mengalami peningkatan dalam kurun waktu lima tahun terakhir. Prinsip utama pencegahan munculnya penderita thalasemia baru adalah dengan mencegah kelahiran bayi thalasemia. Program yang efektif untuk menanggulangi masalah tersebut adalah dengan pendidikan publik penyakit thalasemia. Kegiatan program yang dapat menjadi solusi bagi permasalahan tersebut adalah kegiatan edukasi penyakit thalassemia pada mahasiswa.   Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pemahaman mahasiswa tentang penyakit thalasemia sehingga dapat mendukung program pemerintah untuk menurunkan angka kejadian kelahiran thalasemia. Metode kegiatan ini dilakukan dengan sosialisasi penyakit thalassemia yang diikuti  oleh 82 mahasiswa STIKes Muhammadiyah Ciamis, Jawa Barat yang dilakukan secara daring. Tahapan kegiatan meliputi pembuatan buku digital thalasemia, penyebaran informasi kegiatan, pengerjaan pre test, sesi pemaparan materi, dan sesi evaluasi. Hasil kegiatan pengabdian masyarakat ini memperlihatkan kenaikan nilai rerata dan median post test yaitu 34% dan 50%. Hal ini menunjukkan setelah kegiatan ini terjadi peningkatan pemahaman mahasiswa terhadap penyakit thalasemia. Intervensi berupa edukasi dengan media buku digital dapat meningkatkan pemahaman terhadap penyakit thalasemia. Kata kunci: edukasi; thalasemia; buku digital; pelajar ABSTRACTThe increasing prevalence of thalassemia in Indonesia needs to be alerted. The main principle of preventing the emergence of new thalassemia sufferers is to prevent the birth of thalassemic babies. Effective programs to reduce the prevalence are public education, career screening, counseling, and information about the diagnosis. This community service activity aims to increase student understanding regarding thalassemia disease so that it can support government programs in reducing the incidence of thalassemia births. This community service was attended by 82 students of STIKes Muhammadiyah Ciamis, West Java. This community service was conducted online. There are several stages carried out in this activity, namely the creation of a thalassemia digital book, dissemination of activity information, pre-test work, material presentation sessions, and evaluation sessions. The results of this community service activity raised the average and median scores for the post-test, namely 66.97 and 80. This shows that after these activities there was an increase in students' understanding of thalassemia disease. Interventions in the form of education with ebook media can increase understanding of thalassemia disease. Keywords: education; thalassemia; ebook; student
MEDIA ALTERNATIF KACANG TANAH UNTUK PERTUMBUHAN BAKTERI Mily Zamilah; Undang Ruhimat; Doni Setiawan
Journal of Indonesian Medical Laboratory and Science Vol 1 No 1: Oktober 2020
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v1i1.11

Abstract

The high cost of instant media such as nutrient agar encourages researchers to find alternative media from raw materials that are readily available and cheap. The composition of the media, which is very important for the growth of bacteria, one of which is protein. The content can be obtained from nuts, one of which is groundnut (Arachis hypogaea L). This study aims to determine the growth of Staphylococcus aureus and Escherichia coli bacteria on peanut media which are used as alternative media for Nutrient Agar (NA) substitution. The research method used is Total Plate Count. Escherichia coli bacterial colonies that grew on peanut media were 43 CFU, and colonies that grew on NA media were 238 CFU. While the growth of Staphylococcus aureus bacterial colonies in peanut media was 32 CFU and bacterial colonies growing on NA media were 217 CFU. The conclusion is that peanut media can be used as an alternative media with the growth of Escherichia coli and Staphylococcus aureus bacteria that are in accordance with Standard Plate Count.
APLIKASI METRIK SIGMA DALAM PEMANTAPAN MUTU INTERNAL PADA PEMERIKSAAN UREUM DISALAH SATU LABORATORIUM RUMAH SAKIT KABUPATEN PANGANDARAN Rizal Apriansyah Pratama; Dewi Kania Yulianti; Doni Setiawan
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.64

Abstract

Intralaboratory Quality Control in clinical laboratories is significant and helps detect errors early to improve quality and guarantee examination results. The guaranteed method can be done by applying the sigma metric, which can detect 690,000 errors per million opportunities or 30.9 probability without defects with a minimum sigma value category of 3 and a maximum sigma value of 6. Urea is one of the clinical chemistry examination parameters that often has a sigma value <3. This study aims to determine the application of the sigma metric in internal quality assurance on urea examination in one of the Pangandaran district hospital laboratories. This research method is a descriptive-analytic based on the sigma value in internal quality assurance on urea examination in Pangandaran district hospital laboratories. Based on the study results, the value of sigma 3 - < 4 or marginal in March and May 2021 at the average control level while at the pathological control level increased from 2 sigma in March to 3 sigma in May 2021 said to be marginal.
KONSENTRASI ANTI-HBs PADA KARYAWAN RUMAH SAKIT YANG TELAH MELAKUKAN VAKSINASI HEPATITIS B Doni Setiawan
Masker Medika Vol 11 No 1 (2023): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v11i1.518

Abstract

Introduction: Anti-HBs (Anti HepatitisLBLsurface) is an antibodyLto hepatitisLB that arises from the administration of the hepatitis B vaccine. Hospital employees areLat high riskLof contracting the hepatitis B virus, because they often have direct contact with patients, body fluids and medical equipment infected. ThisLstudy aims to determine the presence of hepatitis B antibodies in Ciamis Hospital employees after three years of vaccination. Method: ThisLstudy usedLa qualitativeLdescriptive methodLand was conducted in 2019. The population was 30 Ciamis Hospital employees who had received Hepatitis B vaccination three years previously. The instrument used was an examination using the ELISA (Enzyme-LinkedLImmunosorbent Assay) Lmethod. Results: LThe resultsLshowedLthat theLaverage levelLof Anti-HBs in employees was 42.46 mIU/mL which showed a positive result with an anti-HBs titer of 10 – 100 mIU/mL (normo-response). Conclusion: Thirty employees of Ciamis Hospital have good immunity, so hepatitis B antibodies can survive after three years of vaccination.
Overview of Anti Streptolysin O Test Results in Postpartum Fever Mothers in the Ciamis Region Ary Nurmalasari; Rohaidah Rohaidah; Doni Setiawan
Nurul Ilmi : Journal of Health Sciences and Midwifery Vol. 1 No. 2 (2023): Nurul Ilmi: Journal of Health Sciences and Midwifery (September 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/nuri.v1i2.366

Abstract

Introduction: Puerperal fever is a symptom of postpartum infection. Symptoms of fever during puerperium often occur due to infection with group A Streptococcus hemoliticus bacteria. Objective: The purpose of this study was to determine the description of the results of anti-streptolysin o examination in mothers for the diagnosis of fever in the puerperium. Method: This study is a descriptive study conducted from January to May 2023 with 40 respondents who were in the postpartum period in the Ciamis Health Center working area. The instrument used was the slide method ASTO examination. The data collection technique was based on Accidental sampling which was then processed and presented descriptively in tabular form and explained in narrative. Result: The results of the Anti Streptolysin O examination of 40 respondents showed positive results as many as 2 people (5%) and 38 people (95%) with negative results. Conclusion: It can be concluded that mothers who are in the postpartum period in the Ciamis Health Center working area have negative Anti Streptolysin O average results.
Erythrocyte Index and Mentzer Index in Fathers of Thalassemia Patients Rhama Adiguna; Doni Setiawan; Undang Ruhimat; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.219

Abstract

Background & Objective Thalassemia is a blood disorder caused by genetic factors that cause the globin chain in hemoglobin to not function normally. It is the most common hereditary disease in the world and in Ciamis Regency in 2021 there were 191 patients. Thalassemia prevention is carried out by screening to identify thalassemia carriers. Hematological examination in the form of erythrocyte index (MCV, MCH, MCHC) and mentzer index has been known to determine thalassemia screening.  The purpose of this study was to determine the description of erythrocyte index and mentzer index in thalassemia patient's father. Method The method used in this study is descriptive method. The population in this study were members of POPTI (Parents Association of Thalassemia Patients) Ciamis Regency. The sample in this study were 30 samples. Result Based on the results of the study obtained the results of erythrocyte index (MCV, MCH) low (MCHC) normal and Mentzer index obtained the results of 20 samples (66%) suspected as thalassemia carrier, 5 samples (17%) suspected iron deficiency anemia and according to the reference value there were 5 samples (17%). Conclusion The results of a study conducted on 30 fathers of thalassemia patients showed that 83% of fathers of thalassemia patients had MCV and MCH values below the normal threshold and 73% had normal MCHC values. Furthermore, from the results of the Mentzer index examination, it is known that 66% of the fathers of thalassemia patients are suspected of having minor thalassemia, 17% are suspected of having iron deficiency anemia disorders and 17% have a normal Mentzer index.