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All Journal Jurnal Biodjati BIOEDUSCIENCE
Jahanzaib Ali
Department of Biotechnology, Faculty of Life Sciences, University of Okara, Renala Khurd
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education

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Novel CRISPR-Cas9 approach to combat with COVID-19 Jahanzaib Ali
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12275

Abstract

Background: The recent outbreak of Corona Virus has affected people all over the world so that WHO declared it a pandemic. This pandemic has become a major problem for people, health professionals, and research institutes that how to combat SARS-CoV-2. The objective of this review article is to provide a brief study that CRISPR/Cas9 can be used to combat SARS-CoV-2. Methods: The comparison of three genome editing techniques (ZNF, TALEN, and CRISPR/Cas9) is done. The mechanism of action of CRISPR/Cas9 is discussed. Results: The studies provided in this review article suggest that it is difficult to recognize this virus as they have their own metabolic machinery and they replicate their selves in the host cell and consume host cellular products to perform their own functions. The COVID-19 proteins may contain some specific areas that are suitable targets for therapy, such as tiny inhibitor molecules for viral polymerase, or impede the attachment of viruses to the receptor sites, for example, viral coat proteins can be used for vaccination purposes. Conclusion: CRISPR-Cas9 can be used to control the SARS-CoV-2 genome from replication and spread to other parts of the body as it can edit the genome quite efficiently. This proposed model will help in targeting the SARS-CoV-2 genome more precisely in the future.
Evaluation of ISSR Primer Applicability for Interspecific Differentiation of Marchantia from West Java Susilo Susilo; Maryanti Setyaningsih; Suci Lestari; Jahanzaib Ali
Jurnal Biodjati Vol 11 No 1 (2026): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v11i1.54766

Abstract

Liverworts of the genus Marchantia play important ecological roles in humid tropical ecosystems; however, molecular marker evaluation for tropical taxa remains limited. This study aimed to assess the amplification performance and cross-species applicability of selected Inter Simple Sequence Repeat (ISSR) primers for interspecific molecular differentiation of Marchantia species from West Java, Indonesia. Four species (M. emarginata, M. geminata, M. paleacea, and M. polymorpha) were analyzed using five ISSR primers. Genomic DNA was extracted using a modified CTAB protocol and amplified through single-primer ISSR-PCR assays. Amplification profiles were scored as the presence or absence of dominant bands. Three primers (F218, F231, and F209) generated clear and reproducible multi-locus banding patterns, producing a total of 21 scorable fragments, whereas BS537 and BS3 failed to amplify detectable products. Polymorphism Information Content (PIC), interpreted as an index of band discrimination capacity across species, ranged from 0.70 to 0.91, with F209 and F218 showing the highest informativeness. The results demonstrate that the selected ISSR primers enable reliable interspecific discrimination among the taxa examined and provide a practical framework for preliminary molecular screening of tropical bryophytes. These findings support the use of validated ISSR primers in future taxonomic and comparative genetic studies of non-model liverwort species.
Co-Authors Maryanti Setyaningsih Suci Lestari Susilo Susilo