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All Journal Buletin Peternakan Pendas : Jurnah Ilmiah Pendidikan Dasar Livestock and Animal Research
Setya Budhi Udrayana
POLITEKNIK PEMBANGUNAN PERTANIAN MALANG
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary Other

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Semen Quality of KUB Chicken in Ringer Lactate-Egg Yolk Diluent with Carrot Extract Supplementation During Cold Storage Udrayana, Setya Budhi; Iswati, Iswati; Bintari, Intan Galuh; Bakar, Abu
Buletin Peternakan Vol 49, No 2 (2025): BULETIN PETERNAKAN VOL. 49 (2) MAY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i2.99638

Abstract

This study aimed to determine the quality of chicken semen from Kampung Unggulan Balitbangtan (KUB) in Ringer lactate-egg yolk diluent with carrot extract supplementation during cold storage. A total of 6 KUB males were selected for the experiment and each of them was subjected to 5 collections of semen. The experiment was performed using a Randomized Block Design (RBD) with 4 treatments. These treatments included P0 (100% Ringer lacate-egg yolk without carrot extract), P1 (80% Ringer lacate-egg yolk + 20% carrot extract), P2 (60% Ringer lacate-egg yolk + 40% carrot extract), P3 (Ringer lactate yolk 40% + 60% carrot extract). The research variables consisted of motility, viability, abnormalities of spermatozoa, and plasma membrane integrity. Data were analyzed using Analysis of Variance (ANOVA) followed by Duncan's test. It was discovered that the percentage of motility and viability up to 48 hours of storage showed significant differences (p<0.05) between treatments. The best results were in group P2, with motility and viability being 40.33 ± 6.60% and 60.86 ± 7.84%, respectively. Abnormalities of spermatozoa up to 24 hours of storage showed significant differences (p<0.05) between treatments, and not significantly different (p>0.05) at 48 hours of storage. Furthermore, plasma membrane integrity failed to show a significant difference between groups at 1 hour of storage and was significantly different (p<0.05) at 24 and 48 hours of storage. The best or the highest result is in the P2 group, with plasma membrane integrity being 71.89 ± 2.40%. It was concluded that the use of 40% carrot extract supplementation in Ringer lacate-egg yolk diluent tends to maintain the quality of KUB chicken semen in cold storage for up to 48 hours and was suitable for the application of artificial insemination.
Evaluating sperm quality characteristics obtained through the female teaser method in native chicken breeds Udrayana, Setya Budhi; Kasianto, Kasianto; Iswati, Iswati; Bintari, Intan Galuh
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.66495

Abstract

Objective: The quality and quantity of chicken sperm are critical factors influenced by the semen collection method. Therefore, this study aims to evaluate the sperm quality obtained from three native chicken breeds using the female teaser method.Methods: These three native chicken breeds are Arabic, Birma, and Kampung Unggul Balitbangtan (KUB) chickens. Each breed comprised a group of three roosters, and the semen collection was performed ten times. Macroscopic and microscopic evaluations were conducted to assess the chicken sperm. The collected data were analyzed using Analysis of Variance (ANOVA) followed by Duncan's multiple range test (DMRT).Result: The results of this study showed significant differences (P<0.05) in the sperm volume among the chicken breeds, with Birma (0.51 ml) and KUB chickens (0.60 ml) exhibiting distinct volumes compared to Arabic chicken (0.25 ml). Generally, the chicken sperm exhibited a milky white color and thick consistency. Microscopic evaluation yielded a mass motility rating of 3+. Meanwhile, individual motility and viability did not exhibit significant differences (P>0.05) among the three native chicken breeds. Abnormalities displayed significant variations (P<0.05), with Birma chicken showing the highest abnormality rate at 9.77%± 0.28. Sperm concentration did not exhibit significant differences (P>0.05), with the highest concentration observed in Arabic chicken at 4265.33±59.84 (x106 cells/ml). However, total sperm count and total motile sperm count exhibited significant variations (P<0.05), with the highest values recorded in KUB chicken at 2241.82±264.13 (x106 cells) and 1936.27±169.85 (x106 cells), respectively.Conclusions: In conclusion, the semen collection method using the female teaser yielded native chicken sperm with high quality and quantity and meets the required standards for successful insemination. This method holds potential and is recommended for application in native chicken breeding programs.
“PERAN PROBIOTIK LACTOBACILLUS DALAM PRODUKSI TERNAK BERKELANJUTAN: TINJAUAN SISTEMATIS TERHADAP EFIKASI BIOLOGIS DAN PENINGKATAN PERFORMA” Riyanto; Kharisma Putriani; Septian Nur Fauzy; Ayu Cahya Risquna; Setya Budhi Udrayana
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 02 (2026): Volume 11 Nomor 02, Juni 2026 Published
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i02.47816

Abstract

Antibiotic use in livestock production contributes significantly to antimicrobial resistance, creating urgency for more sustainable alternatives. Lactobacillus-based probiotics have been widely explored, but their effectiveness varies depending on livestock species, strain, dosage, and rearing conditions, highlighting the need for comprehensive evaluation. This systematic review aimed to assess the role of Lactobacillus in suppressing pathogenic bacteria, improving animal performance, and its potential as an alternative to antibiotics or antibiotic growth promoters (AGPs). The study applied a Systematic Literature Review approach using the PICO framework and PRISMA guidelines. Literature searches were conducted in Scopus, Web of Science, PubMed, and ScienceDirect, focusing on empirical studies published in the last 10 years. Study quality was assessed using MMAT, and data were synthesized through thematic analysis with NVivo 14. Out of 106 identified records, 61 studies met the inclusion criteria. The findings indicate that Lactobacillus suppresses pathogens through competitive exclusion, acidification of the gut environment, and production of antimicrobial metabolites. Evidence of improved performance was strongest in broilers and pigs, particularly in weight gain and feed efficiency, while layers showed improvements in egg production. However, variability across studies suggests context-dependent outcomes. Overall, Lactobacillus shows strong potential to reduce antibiotic reliance, though further research is needed to standardize applications and validate its effectiveness across production systems.
Co-Authors Abu Bakar Ayu Cahya Risquna Intan Galuh Bintari Iswati Iswati, Iswati Kasianto, Kasianto Kharisma Putriani Riyanto Septian Nur Fauzy