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All Journal HAYATI Journal of Biosciences
Hayati Hayati
Department of Pharmaceutical Biology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, Jakarta 13460, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Microscopical Evaluation and TLC Analysis of Pluchea indica (L.) Less: Leaf, Stem, and Root Ni Putu Ermi Hikmawanti; Fadlina Chany Saputri; Arry Yanuar; Ratih Asmana Ningrum; Abdul Mun'im; Hayati Hayati
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.71-81

Abstract

Pluchea indica (L.) Less is traditionally utilized to treat postpartum women in Indonesia. The plant has many pharmacological properties, so that it can be further developed as herbal medicine. In that development process, plant authentication is needed to ensure the quality of raw materials. A simple microscopical and thin-layer chromatography (TLC) analysis might be a way to authenticate the plant, but it has never been reported. So, this study evaluates the microscopical and TLC analysis for P. indica authentication in standardized herbal medicines production. Plant microscopic observation, fluorescence analysis, and polyphenol screening were conducted. n-Hexane, ethyl acetate, and methanol extracts of plant organs were then analyzed by TLC. Here, we reported that in microscopical analysis the simplicia of P. indica contains trichomes and tannin-containing cells. In addition, chlorogenic acid as a marker was present in TLC analysis by ethyl acetate-water-formic acid-acetic acid (8.5:1.5:1:1, v/v). The results of this evaluation might provide additional information in the identification, authentication, and quality control of P. indica as a raw material for herbal medicine.
Co-Authors Abdul Mun'im Arry Yanuar Fadlina Chany Saputri Ni Putu Ermi Hikmawanti Ratih Asmana Ningrum