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All Journal HAYATI Journal of Biosciences International Journal of Agriculture System Jurnal Aplikasi Sains dan Teknologi : Agrisevika
Rika Miftakhul Jannah
Universitas Pembangunan Nasional “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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The Genetic Variability of Indonesian Local Foxtail Millet Accession Based on Agro-morphological Traits and Early Salinity Tolerance Evaluation Utilizing SiDREB2-based SNAP Marker Sri Ratnawati; Rika Miftakhul Jannah; Yuli Irvana Dewi; Ramadaniarto Rizqullah; Willy Bayuardi Suwarno; Sintho Wahyuning Ardie
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.82-93

Abstract

Foxtail millet (Setaria italica L. Beauv.) is a grain-producing crop with high nutritional benefits and adaptability to broad environmental conditions. Indonesian local foxtail millet accessions are valuable genetic resources for crop improvement of the species. This study aims to assess the biodiversity of Indonesian local foxtail millet accessions and estimate their salinity tolerance level utilizing the SiDREB2-based SNAP marker and early evaluation at the seedling stage. Our results showed that eight Indonesian local foxtail millet accessions, namely Toraja, Mauliru-2, Hambapraing, NTB-1, ICERI-5, ICERI-6, Botok-4, and Botok-10, had high variability based on agro-morphological traits. The Hambapraing and Mauliru-2 were the potential accessions with high yield estimates (2.33 ton.ha-1 and 1.93 ton.ha-1) and early harvest time (<110 DAT). ICERI-6 was indicated as a salinity-tolerant genotype based on the SiDREB2-based marker and early salinity evaluation at the seedling stage, while Toraja, Mauliru-2, NTB-1, and Botok-4 were categorized as sensitive genotypes. Germplasm variability and the estimated salinity tolerance level of Indonesian local foxtail millet accessions presented in this study serve as essential information in the foxtail millet breeding for high productivity, early harvest time, and salinity tolerant variety.
Penghijauan Sekolah melalui Vertikultur Botol Bekas dan Penanaman Trembesi sebagai Edukasi Lingkungan di SDN Bendungan, Jombang Rika Miftakhul Jannah; Arga Dwi Indrawan; Makhziah Makhziah
Jurnal Aplikasi Sains dan Teknologi : Agrisevika Vol. 3 No. 1 (2026): Juni 2026
Publisher : Faculty of Agriculture, Universitas Pembangunan Nasional "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/agrisevika.v3i1.28

Abstract

Program penghijauan berbasis vertikultur dan penanaman trembesi dilaksanakan di SDN Bendungan, Kabupaten Jombang, sebagai bentuk implementasi pendidikan lingkungan hidup berbasis aksi nyata. Kegiatan ini bertujuan menciptakan lingkungan sekolah yang hijau, sehat, dan edukatif sekaligus meningkatkan partisipasi aktif siswa dalam pelestarian lingkungan. Metode yang digunakan meliputi ceramah interaktif, pengisian kuesioner, dan praktik langsung pembuatan instalasi vertikultur menggunakan botol bekas serta penanaman pohon trembesi (Samanea saman). Sasaran kegiatan adalah siswa kelas 1 hingga 6 dengan pendampingan guru dan tim pelaksana. Hasil kegiatan menunjukkan antusiasme tinggi dari siswa. Berdasarkan hasil kuesioner, seluruh peserta menyatakan memperoleh pengetahuan baru mengenai vertikultur dan pemanfaatan limbah plastik sebagai media tanam. Selain itu, kegiatan ini mendukung prinsip ekonomi sirkular dan berkontribusi terhadap pencapaian Tujuan Pembangunan Berkelanjutan (SDGs) poin 13 (Penanganan Perubahan Iklim) dan poin 15 (Ekosistem Darat). Program ini menunjukkan bahwa pendekatan edukatif dan partisipatif melalui praktik vertikultur efektif dalam menumbuhkan kesadaran ekologis sejak dini dan membentuk karakter peduli lingkungan di lingkungan sekolah dasar.
Comparison of 2,4-D Alone and 2,4-D–Kinetin Combination on Callus Formation in Apium graveolens and Mentha sp. Rika Miftakhul Jannah
International Journal of Agriculture System VOLUME 13 ISSUE 2, DECEMBER 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v13i2.6762

Abstract

Celery (Apium graveolens L.) and mint (Mentha sp.) are economically important aromatic crops. However, their propagation through seeds and vegetative methods is often inefficient due to low seed viability, inconsistent germination, contamination risks, and poor plantlet uniformity. Therefore, this study assessed the response of two plant growth regulator formulations for callus induction in both species under in vitro conditions using a descriptive analysis. Two media treatments were tested: MD1 (Murashige and Skoog (MS) + 1.2 mg L-1 2,4-D) and MD2 (MS + 0.3 mg L-1 2,4-D + 0.5 mg L-1 kinetin). Observations were conducted on days 7, 14, 21, and 35 after induction (DAI) to assess explant survival, callus formation, and morphology. MD2 medium showed a tendency toward higher explant survival and greater callus formation percentages than MD1 in both species under the observed conditions. Callus initiation occurred between 7 and 14 DAI, with MD2 producing a greater proportion of viable and actively proliferating calli, predominantly characterized by friable-to-compact textures and lighter coloration. In contrast, explants cultured on MD1 exhibited a higher incidence of tissue browning and reduced callus viability, indicating the inhibitory effects associated with high auxin concentrations. These results suggest that a balanced auxin–cytokinin combination provides a more favorable hormonal environment for early callus induction than a single high-dose auxin treatment alone. Therefore, MD2 medium is recommended as an optimal formulation for early-stage callus induction in celery and mint, with potential applications in micropropagation.
Co-Authors Arga Dwi Indrawan Makhziah Ramadaniarto Rizqullah Sintho Wahyuning Ardie Sri Ratnawati Willy Bayuardi Suwarno Yuli Irvana Dewi