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Prospect of Utilization of Microorganisms Streptomyces sp. and Trichoderma sp. in Supporting Sustainable Agriculture in the Age of Modern Agriculture Arga Dwi Indrawan; Penta Suryaminarsih; Tri Mujoko
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1506

Abstract

The geographical condition of Indonesia's agriculture in the tropics supports agricultural business throughout the year through agricultural intensification. Agricultural intensification brings benefits to agricultural productivity, but also causes farmers to be dependent on pesticides and inorganic fertilizers in excess, causing long-term degradation of agricultural land productivity. Susstainable Agriculture is an approach to increase agricultural productivity while still being friendly to the agricultural ecosystem. Sustainable agriculture can be achieved through the preservation of agricultural resources, one of which is the use of beneficial microorganisms. Stre ptomyces sp. and Trichoderma sp. is a multi-role microorganism as a supporter of plant growth, biological agent in pest control, and helps increase soil fertility. The use of these microorganisms has good prospects for sustainable modern agriculture in order to achieve food security but also has obstacles that need to be overcome through further research.
KARAKTER FISIK DAN KIMIA DIGESTAT TIGA JENIS KOTORAN TERNAK PADA KONDISI ANAEROB Saefur Rohman; Arga Dwi Indrawan
Plumula : Berkala Ilmiah Agroteknologi Vol. 11 No. 1 (2023): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Limbah kotoran ternak selaku produk samping budidaya peternakan mempunyai dampak negatif bagi lingkungan berupa bau, cemaran lingkungan, dan sumber penyakit. Biokonversi secara anaerob dapat mengonversi limbah kotoran ternak menghasilkan digestat akhir berbentuk slurry yang kaya kandungan hara sebagai pupuk organik. Sejauh ini, studi tentang dinamika sifat fisik dan kimia digestat dari berbagai jenis kotoran ternak masih jarang dilakukan. Penelitian ini bertujuan untuk mengkaji pengaruh tiga jenis kotoran ternak (kambing, ayam, dan sapi) terhadap sifat fisik dan kimia digestat. Percobaan dirancang menggunakan rancangan acak lengkap (RAL) sederhana dengan perlakuan tiga jenis kotoran dengan tiga kali ulangan. Digestat dibuat dengan bahan kotoran ternak, urin sapi, aktivator EM4, dan daun lamtoro yang diinkubasikan secara anaerob selama 14 hari. Parameter yang diamati meliputi aroma, warna, kekentalan, buih, pH, dan suhu yang diamati 2 hari sekali hingga 14 hari setelah inkubasi (HSI). Hasil menunjukkan bahwa digestat kambing dan sapi memiliki kesamaan pola dinamika sifat fisik dan kimia. Karakter aroma, warna, kekentalan, dan buih digestat sapi dan kambing cenderung tidak berubah selama masa inkubasi dibandingkan digestat ayam. pH ketiga digestat memiliki kecenderungan untuk turun di awal inkubasi dan naik di pertengahan hingga akhir inkubasi mendekati pH 7,0. Kenaikan suhu digestat ayam lebih tinggi mencapai 40 oC sementara kenaikan suhu digestat kambing dan sapi lebih rendah. Secara keseluruan, digestat kambing dan sapi cenderung lebih sulit didegradasi dan membutuhkan waktu lebih lama untuk terdekomposisi sempurna dibandingkan digestat ayam yang diduga dikarenakan perbedaan karakter kotoran yang dipengaruhi komposisi pakan, mikroba usus, dan struktur pencerbaan ternak tersebut.
In Vitro Evaluation of Trichoderma spp. against Sugarcane Eye Spot Disease (Bipolaris sp.) Laksamana Agadhia Raharjo; Arga Dwi Indrawan; Noni Rahmadhini
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 1 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.1.143

Abstract

Sugar cane (Saccharum officinarum L.) is the main ingredient in sugar production. Sugarcane is widely cultivated in warm and tropical areas and is widely used as a sweetener because it contains a lot of fructose and glucose. National sugar production shows a decline, the majority of which is caused by pathogens, especially the fungus Bipolaris sp. capable of producing up to 85% damage per Ha if no control is applied. Biological control using Trichoderma spp. can control this pathogen and is able to promote sugarcane growth. The purpose of this study was to determine the effectiveness of Trichoderma spp. in controlling Bipolaris sp. in vitro. The results showed isolates TD1, TD2, TD3 were able to inhibit Bipolaris sp. antagonistically and volatilely, the antagonist test showed a result of 86% (TD1) while the volatile test of 65% (TD2). Conclusion Trichoderma spp. can inhibit the growth of Bipolaris sp. on an in vitro scale. Keywords: BCA, Bipolaris sp. Trichoderma spp. ABSTRAK Tebu (Saccharum officinarum L.) merupakan bahan utama dalam pembuatan gula. Tebu banyak dibudidayakan didaerah hangat dan tropikal dan banyak digunakan sebagai pemanis dikarenakan mengandung banyak fruktosa dan glukosa. Produksi gula nasional menunjukan adanya penurunan yang mayoritas disebabkan oleh patogen terutama jamur Bipolaris sp. yang mampu menghasilkan kerusakan hingga 85% per Ha jika tidak diberlakukan pengendalian. Pengendalian biologis menggunakan Trichoderma spp. dapat mengendalikan patogen ini dan mampu mendorong pertumbuhan tebu. Tujuan penelitian ini ialah untuk mengetahui efektivitas Trichoderma spp. dalam mengendalikan Bipolaris sp. secara In vitro. Hasil menunjukkan isolat TD1, TD2, TD3 mampu menghambat Bipolaris sp. secara antagonis dan volatile, uji antagonis memperlihatkan TD1 memiliki hasil 86% sementara uji volatile TD2 memiliki hasil 65%. Kesimpulan Trichoderma spp. dapat menghambat pertumbuhan Bipolaris sp. dalam skala In vitro. Kata kunci: APH, Bipolaris sp. Trichoderma spp.
Prospect of Utilization of Microorganisms Streptomyces sp. and Trichoderma sp. in Supporting Sustainable Agriculture in the Age of Modern Agriculture Arga Dwi Indrawan; Penta Suryaminarsih; Tri Mujoko
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1506

Abstract

The geographical condition of Indonesia's agriculture in the tropics supports agricultural business throughout the year through agricultural intensification. Agricultural intensification brings benefits to agricultural productivity, but also causes farmers to be dependent on pesticides and inorganic fertilizers in excess, causing long-term degradation of agricultural land productivity. Susstainable Agriculture is an approach to increase agricultural productivity while still being friendly to the agricultural ecosystem. Sustainable agriculture can be achieved through the preservation of agricultural resources, one of which is the use of beneficial microorganisms. Stre ptomyces sp. and Trichoderma sp. is a multi-role microorganism as a supporter of plant growth, biological agent in pest control, and helps increase soil fertility. The use of these microorganisms has good prospects for sustainable modern agriculture in order to achieve food security but also has obstacles that need to be overcome through further research.
The Potency of Streptomyces spp. from Shallot Land as Entomopathogen of Onion Caterpillar Pest Spodoptera exigua Pratista, Ariq Dies; Windriyanti, Wiwin; Suryaminarsih, Penta; Mujoko, Tri; Indrawan, Arga Dwi; Harsiyanti, Harsiyanti
International Journal on Food, Agriculture and Natural Resources Vol 6, No 4 (2025): IJ-FANRES
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v6i4.569

Abstract

Spodoptera exigua is a major pest of shallots (Allium ascalonicum L.), capable of causing up to 100% yield loss if uncontrolled. Streptomyces sp. is a soil-dwelling Actinomycetes bacterium that can be used to control S. exigua. This study aimed to determine the potential of Streptomyces spp. isolates from shallot fields as an entomopathogen against S. exigua. The research employed a Factorial Complete Randomized Design (CRD). The first factor consisted of one Streptomyces spp. isolate obtained from the Pare-Kediri region (BMP) and three isolates from Sidera-Palu (BMS1, BMS2, and BMS3). The second factor was the concentration of the Streptomyces sp. suspension consist of 0%; 5%; 10%; and 15% with three replication per treatment (48 units total). Data on larval mortality and damage intensity were analyzed using RStudio via ANOVA and DMRT (α=5%). The BMP (15%) treatment demonstrated the highest efficacy, achieving 76.67% larval mortality while maintaining the lowest damage intensity of 0.20% on shallot plants. These results highlight BMP isolate at 15% concentration as the most promising biocontrol agent and support its further field evaluation as a sustainable alternative to chemical insecticides.
Penghijauan Sekolah melalui Vertikultur Botol Bekas dan Penanaman Trembesi sebagai Edukasi Lingkungan di SDN Bendungan, Jombang Rika Miftakhul Jannah; Arga Dwi Indrawan; Makhziah Makhziah
Jurnal Aplikasi Sains dan Teknologi : Agrisevika Vol. 3 No. 1 (2026): Juni 2026
Publisher : Faculty of Agriculture, Universitas Pembangunan Nasional "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/agrisevika.v3i1.28

Abstract

Program penghijauan berbasis vertikultur dan penanaman trembesi dilaksanakan di SDN Bendungan, Kabupaten Jombang, sebagai bentuk implementasi pendidikan lingkungan hidup berbasis aksi nyata. Kegiatan ini bertujuan menciptakan lingkungan sekolah yang hijau, sehat, dan edukatif sekaligus meningkatkan partisipasi aktif siswa dalam pelestarian lingkungan. Metode yang digunakan meliputi ceramah interaktif, pengisian kuesioner, dan praktik langsung pembuatan instalasi vertikultur menggunakan botol bekas serta penanaman pohon trembesi (Samanea saman). Sasaran kegiatan adalah siswa kelas 1 hingga 6 dengan pendampingan guru dan tim pelaksana. Hasil kegiatan menunjukkan antusiasme tinggi dari siswa. Berdasarkan hasil kuesioner, seluruh peserta menyatakan memperoleh pengetahuan baru mengenai vertikultur dan pemanfaatan limbah plastik sebagai media tanam. Selain itu, kegiatan ini mendukung prinsip ekonomi sirkular dan berkontribusi terhadap pencapaian Tujuan Pembangunan Berkelanjutan (SDGs) poin 13 (Penanganan Perubahan Iklim) dan poin 15 (Ekosistem Darat). Program ini menunjukkan bahwa pendekatan edukatif dan partisipatif melalui praktik vertikultur efektif dalam menumbuhkan kesadaran ekologis sejak dini dan membentuk karakter peduli lingkungan di lingkungan sekolah dasar.
Biocontrol Strategies Using Streptomyces spp. and Trichoderma spp. against the Sugarcane Smut Pathogen Sporisorium scitamineum: An in Vitro Study Indrawan, Arga Dwi; Raharjo, Laksamana Agadhia; Rahmadhini, Noni
PLANTA TROPIKA Vol. 14 No. 1 (2026)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v14i1.21414

Abstract

Sporisorium scitamineum is a Basidiomycota pathogen that causes smut disease in sugarcane plants. The pathogen can infect all types of sugarcane, with the extent of damage depending on the plant's susceptibility. Farmers rely on chemical pesticides to control S. scitamineum , but these pesticides have negative environmental impacts. Using Trichoderma sp. and Streptomyces sp. as biocontrol has been proven against fungal infections and is safe for the environment. This study evaluated the potential of Trichoderma sp. and Streptomyces sp. against S. scitamineum under in vitro conditions. Each of the three isolates of Trichoderma sp. and Streptomyces sp. was assessed using the VOC (Volatile Organic Compound), chitinase enzyme, and in vitro antagonistic assays. The results indicated that Streptomyces spp. demonstrated higher chitinase activity compared to Trichoderma spp., with isolate ST1 having the highest chitinase index at 3.7%. VOC test demonstrated that Streptomyces spp. markedly inhibited the mycelial growth of S. scitamineum, attaining inhibition rates of up to 82%. Conversely, Trichoderma spp. exhibited enhanced antagonistic activity via direct interaction, with isolate TD2 suppressing pathogen growth by 82% in dual-culture assays. The findings show that Streptomyces spp. and Trichoderma spp. suppressed S. scitamineum in different but complementary ways. The study shows that they could be used as environmentally friendly biological control agents to manage sugarcane smut.
Potensi Trichoderma spp. dari Koleksi Tanah Tanaman Perkebunan asal Jawa Timur sebagai Antagonis Fusarium oxysporum Khansa Amara; Arga Dwi Indrawan; Af’idzatuttama
Plumula : Berkala Ilmiah Agroteknologi Vol. 13 No. 2: Juli 2025
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v13i2.261

Abstract

Aplikasi Trichoderma spp. hasil eksplorasi pada tanah tanaman sehat dapat berpotensi dalam pengendalian penyakit layu yang ramah lingkungan dan dapat diterapkan dalam jangka panjang. Penelitian ini bertujuan untuk menilai kualitas spora dan kemampuan antagonis Trichoderma spp. yang diisolasi dari tanah tanaman kakao, kopi, dan cengkeh terhadap F. oxysporum secara in vitro. Penelitian disusun dengan rancangan acak lengkap (RAL), dengan perlakuan jenis Trichoderma dari sumber sampel tanah berbeda. Variabel pengamatan meliputi: kerapatan dan viabilitas spora, serta tingkat hambatan relatif yang selanjutnya dianalisis ragam (ANOVA) menggunakan SAS on Demand, dan uji lanjut Tukey pada taraf 5%. Seluruh isolat Trichoderma spp. menunjukkan hasil melebihi kriteria penilaian standar mutu sebagai agens hayati berdasarkan SNI 8027.3:2014, dengan kerapatan spora 108 spora/ml. Namun, hanya isolat Trichoderma spp. asal tanah tanaman kopi yang memiliki kualitas spora dan persentase penghambatan terbaik dalam menekan pertumbuhan diameter koloni F. oxysporum, yaitu sebesar 59%.